Search Patents
  • CDNA encoding a polypeptide including a hevein sequence
    Patent number: 5399668
    Abstract: A cDNA clone (HEV1) encoding hevein was isolated via polymerase chain reaction (PCR) using mixed oligonucleotides corresponding to two regions of hevein as primers and a Hevea brasiliensis latex cDNA library as a template. HEV1 is 1018 nucleotides long and includes an open reading frame of 204 amino acids. The deduced amino acid sequence contains a putative signal sequence of 17 amino acid residues followed by a 187 amino acid polypeptide. The amino-terminal region (43 amino acids) is identical to hevein and shows homology to several chitin-binding proteins and to the amino-termini of wound-induced genes in potato and poplar. The carboxyl-terminal portion of the polypeptide (144 amino acids) is 74-79% homologous to the carboxyl-terminal region of wound-inducible genes of potato. Wounding, as well as application of the plant hormones abscisic acid and ethylene, resulted in accumulation of hevein transcripts in leaves, stems and latex, but not in roots, as shown by using the cDNA as a probe.
    Type: Grant
    Filed: May 26, 1992
    Date of Patent: March 21, 1995
    Assignee: Board of Trustees operating Michigan State University
    Inventors: Natasha V. Raikhel, Willem F. Broekaert, Nam-Hai Chua, Anil Kush
  • Application of resins consisting of functional polymers as the stationary phase in affinity chromatography for the purification of growth factors, and corresponding purification method
    Patent number: 5122597
    Abstract: The present invention relates to the application, as the stationary phase in affinity chromatography for the purification of the FGF type growth factors, of the polymers or copolymers onto which --SO.sub.3 H and --SO.sub.3 M groups are randomly bound, groups in which M denotes a physiologically acceptable metal, as well as, preferably, --SO.sub.2 R groups, in which R denotes a radical obtained by removal of a hydrogen atom from the amino group of an amino acid or an amino acid derivative.The invention relates also to the obtaining of a purified bFGF or aFGF growth factor, or a mixture of these growth factors, consisting in carrying out an affinity chromatography on a resin such as defined hereinabove, by carrying out an elution with a neutral pH buffer having an ionic strength equivalent to that of a 0.15 M to 2.5 M NaCl solution.
    Type: Grant
    Filed: April 15, 1988
    Date of Patent: June 16, 1992
    Assignee: Centre National de la Recherche Scientifique (CNBS)
    Inventors: Denis S. C. Barritault, Josette Badet nee Genissel, Jose P. Courty, Marie-Anne Dourges nee Jacquot, Danielle Gulino nee Debrac, Jacqueline Jozefonvicz nee Dorgebray
  • Eucaryotic NAD cyclases
    Patent number: 5202426
    Abstract: Eucaryotic NAD cyclases able to cause production of cyclic adenosine diphosphate ribose from nicotinamide adenine dinucleotide.
    Type: Grant
    Filed: December 17, 1990
    Date of Patent: April 13, 1993
    Assignee: Marine Biological Laboratory
    Inventors: Felix Strumwasser, Mark R. Hellmich, David L. Glick
  • Polypeptides and method for preparing the same
    Patent number: 5434245
    Abstract: There is provided a polypeptide having the following formula (I'), and which polypeptide is referred to as Glu-OC and a process for the preparation thereof.
    Type: Grant
    Filed: December 16, 1992
    Date of Patent: July 18, 1995
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Nobuto Koyama, Fusao Kimizuka, Ikunoshin Kato
  • Method for solubilization and naturation of somatotropin
    Patent number: 5064943
    Abstract: A method for solubilization and naturation of somatotropin using an aqueous alkaline solution results in lower dimer formation and eliminates denaturants and separate renaturation steps and agents.
    Type: Grant
    Filed: December 16, 1988
    Date of Patent: November 12, 1991
    Assignee: American Cyanamid Company
    Inventors: Kevin M. McCoy, Robert A. Frost
  • Heparin-binding brain mitogens
    Patent number: 5171842
    Abstract: A group of growth factors, designated heparin-binding brain mitogens (HBBMs), is disclosed. The HBBMs are isolated from brain tissue by a sequence of purification steps.
    Type: Grant
    Filed: November 1, 1991
    Date of Patent: December 15, 1992
    Assignee: American Cyanamid Company
    Inventors: Peter Bohlen, Peter Gautschi-Sova
  • Nitrocellulose filtration to remove proteins from polynucleotides
    Patent number: 5004806
    Abstract: The present invention encompasses a method for removing proteins from a solution containing polynucleotides and proteins comprising filtering the solution through a nitrocellulose membrane at neutral or basic pH.
    Type: Grant
    Filed: October 17, 1988
    Date of Patent: April 2, 1991
    Assignee: Molecular Devices Corporation
    Inventor: Viola T. Kung
  • Process for the preparation of mature human serum albumin
    Patent number: 5187261
    Abstract: Mature human serum albumin is produced from a human serum albumin produced by a microbiological route in the form of fused protein ("pseudo-pro-HSA") containing an N-terminal peptide elongation.
    Type: Grant
    Filed: February 8, 1991
    Date of Patent: February 16, 1993
    Assignee: Genetica
    Inventors: Martine Latta, Jean-Francois Mayaux, Paolo Sarmientos
  • Lymphocyte adhesion receptor for high endothelium, CD44
    Patent number: 5504194
    Abstract: A lymphoid cell line cDNA that encodes an adhesion receptor for high endothelial venules (HEV).
    Type: Grant
    Filed: May 15, 1992
    Date of Patent: April 2, 1996
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: Thomas P. St. John, W. Michael Gallatin, Rejean L. Idzerda
  • Brazzein sweetener
    Patent number: 5346998
    Abstract: Disclosed herein is a protein sweetener that has been isolated from Pentadiplandra brazzeana Baillon. The sweetener is thermostable, lysine rich, and has a relative long lasting taste. Also disclosed is a recombinant host capable of producing the sweetener in large quantities. Compositions of this sweetener with other sweeteners are also disclosed.
    Type: Grant
    Filed: June 29, 1993
    Date of Patent: September 13, 1994
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Bengt G. Hellekant, Ding Ming
  • Muteins of human erythropoietin, the preparation thereof and the use thereof
    Patent number: 5457089
    Abstract: The invention relates to muteins of human erythropoietin (EPO) in the carboxyl terminal region which are prepared by means of recombinant DNA techniques. These mutants have advantageous properties in comparison with human wild-type EPO.
    Type: Grant
    Filed: July 6, 1994
    Date of Patent: October 10, 1995
    Assignee: Behringwerke Aktiengesellschaft
    Inventors: Mathias Fibi, Gerd Zettlmeissl, Hans Kupper
  • Process for protein particle size reduction using a fluid-energy mill
    Patent number: 5021554
    Abstract: The particle size of amorphous protein material is reduced to uniform particulates without protein decomposition or loss of activity by passing the material through a fluid-energy mill.
    Type: Grant
    Filed: February 24, 1989
    Date of Patent: June 4, 1991
    Assignee: Eli Lilly and Company
    Inventor: William W. Thompson
  • Almond N-glycosidase gene
    Patent number: 5710016
    Abstract: An almond N-glycosidase gene and genes associated therewith are described. Vectors integrated such genes therein, recombinant microorganisms transformed with said vectors, and a process for preparing the almond N-glycosidase using said recombinant microorganisms are also described.
    Type: Grant
    Filed: April 7, 1995
    Date of Patent: January 20, 1998
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Hiroyuki Izu, Masanori Mitta, Ikunoshin Kato
  • Method for isolating and purifying transferrin and lactoferrin receptor proteins and vaccines containing the same
    Patent number: 5141743
    Abstract: The present invention relates to a method for isolating and purifying transferrin and lactoferrin receptor proteins from bacterial pathogens by affinity chromatography and to vaccine antigens containing the purified receptor proteins.
    Type: Grant
    Filed: January 10, 1991
    Date of Patent: August 25, 1992
    Assignee: University Technologies International, Inc.
    Inventor: Anthony B. Schryvers
  • Streptolysin O antigen derivatives, its production and uses
    Patent number: 5354846
    Abstract: A non-toxic and non-cytolytic derivative of streptolysin O which retains at least one (preferably immunodominant) epitope is produced, and can be used especially in diagnostic tests for detecting of the presence of antibodies to Streptococcus pyogenes in a sample.
    Type: Grant
    Filed: June 8, 1993
    Date of Patent: October 11, 1994
    Inventor: Michael Kehoe
  • Purification of von Willebrand Factor by affinity chromatography
    Patent number: 5006642
    Abstract: A method of preparing von Willebrand Factor by disassociating it from a chaotropic agent in solution therewith and preferably treating the same under controlled temperature either in liquid or lyophilized form.
    Type: Grant
    Filed: June 13, 1988
    Date of Patent: April 9, 1991
    Assignee: Rhone-Poulenc Rorer Pharmaceuticals Inc.
    Inventors: Jack Newman, David L. Farb
  • Method of folding somatotropins
    Patent number: 4975529
    Abstract: A method of recovering biologically active somatotropins which are produced as insoluble refractile bodies in transformed microorganisms which comprises dissolving refractile bodies in a denaturant and 2-amino-2-methyl-1-propanol, homogenizing, and diluting to fold.
    Type: Grant
    Filed: August 18, 1989
    Date of Patent: December 4, 1990
    Assignee: Monsanto Company
    Inventors: Ronald B. Frazier, Yasuo Konishi
  • Protein active in humoral hypercalcemia of malignancy-PthrP
    Patent number: 5460978
    Abstract: Substantially pure parathyroid hormone related protein (PTHrP) active in humoral hypercalcemia of malignancy and sub-units and fragments thereof. Antibody reagents capable of binding to epitopes of PTHrP. Methods and kits for the detection of PTHrP.
    Type: Grant
    Filed: June 14, 1991
    Date of Patent: October 24, 1995
    Assignee: The University of Melbourne
    Inventors: Thomas J. Martin, Jane M. Moseley, Bruce E. Kemp, Richard E. H. Wettenhall
  • DNA encoding and methods for producing BMP-8 proteins
    Patent number: 5688678
    Abstract: Purified cartilage and/or bone inductive proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.
    Type: Grant
    Filed: November 26, 1991
    Date of Patent: November 18, 1997
    Assignee: Genetics Institute, Inc.
    Inventors: Rodney M. Hewick, Jack H. Wang, John M. Wozney, Anthony J. Celeste
  • Homologs of aprotinin produced from a recombinant host, process, expression vector and recombinant host therefor and pharmaceutical use thereof
    Patent number: 5032573
    Abstract: Microbially produced aprotinin and aprotinin homologs used for treating patients suffering from an excess release of pancreatic elastase, serum elastase or leukocyte elastase.
    Type: Grant
    Filed: October 2, 1989
    Date of Patent: July 16, 1991
    Assignee: Bayer Aktiengesellschaft
    Inventors: Ernst-August Auerswald, Werner Schroder, Eugen Schnabel, Wolfgang Bruns, Gerd Reinhardt, Michael Kotick
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