Abstract: This invention is directed to methods of detecting biofilm treated in situ with an active ingredient, and subsequently imaging or detecting types or varieties of biofilm and/or architectural changes in biofilm when treated with certain actives. Additionally, the invention contemplates screening assays to discover further candidate compounds that can affect biofilm growth and formation, as it relates to the maintenance of oral health and prevention of oral diseases.

Abstract: Methods for visualizing oral biofilm growth and methods for identifying compounds that modulate biofilm growth are provided. Biofilm samples are stained with fluorescent dyes and viewed with a confocal laser scanning microscope. Biofilm samples are in a container with a cover slip configured for viewing the biofilm sample with the confocal laser scanning microscope and the coverslip has a thickness variation of 0.02 mm or less.

Abstract: Methods for visualizing oral biofilm growth and methods for identifying compounds that modulate biofilm growth are provided. Biofilm samples are stained with fluorescent dyes and viewed with a confocal laser scanning microscope. Biofilm samples are in a container with a cover slip configured for viewing the biofilm sample with the confocal laser scanning microscope and the coverslip has a thickness variation of 0.02 mm or less.

Abstract: Method of improving tissue integrity of tissue that functions as a barrier are provided. Method of protecting and maintaining tissue integrity of tissue that functions as a barrier are provided. Method of repairing damage to tissue that functions as a barrier, and to restoring and improving function of such damaged tissue are provided. Methods of improving, maintaining, protecting, repairing and/or restoring the integrity of tissue which functions as a barrier and repair of damage to and healing of wounds to such tissue improves, maintains, protects, repairs and restores immunity. The methods comprising contacting the tissue with an effective amount of a composition comprising one or more sources of zinc ions such as zinc oxide, zinc citrate, or combination thereof zinc oxide and zinc citrate, and optionally further comprising arginine and/or one or more neutral amino acids.