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  • Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
    Patent number: 5814465
    Abstract: The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor b
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: September 29, 1998
    Assignee: Kikkoman Corporation
    Inventors: Hiroki Tatsumi, Satoshi Fukuda, Mamoru Kikuchi, Yasuji Koyama
  • Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
    Patent number: 5843746
    Abstract: The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor b
    Type: Grant
    Filed: January 13, 1997
    Date of Patent: December 1, 1998
    Assignee: Kikkoman Corporation
    Inventors: Hiroki Tatsumi, Satoshi Fukuda, Mamoru Kikuchi, Yasuji Koyama
  • MUTANT LUCIFERASE
    Publication number: 20140170686
    Abstract: An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from Phtotinus pyralis, Luciola mingrelica, Luciola cruciata, Luciola lateralis, Hotaria parvula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia miyako or Photinus pennsylvanica. In the seguence of the recombinant luciferase, the amino acid residue corresponding to phenylalanine 295 in Photinus pyralis wild-type luciferase or to leucine 297 in Luciola mingrelica, Luciola cruciata or Luciola lateralis wild-type luciferases, is mutated compared to the corresponding amino acid which appears in the corresponding wild-type luciferase sequence. The recombinant luciferase has increased thermostability compared to the corresponding wild-type luciferase.
    Type: Application
    Filed: January 17, 2014
    Publication date: June 19, 2014
    Applicant: Promega Corporation
    Inventors: David J. Squirrell, Melenie J. Murphy, Rachel L. Price, Christopher R. Lowe, Peter J. White, Laurence C. Tisi, James A. H. Murray
  • MUTANT LUCIFERASE
    Publication number: 20110177540
    Abstract: An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata, Luciola lateralis, Hotaria parvula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia miyako or Photinus pennsylvanica. In the sequence of the recombinant luciferase, the amino acid residue corresponding to phenylalanine 295 in Photinus pyralis wild-type luciferase or to leucine 297 in Luciola mingrelica, Luciola cruciata or Luciola lateralis wild-type luciferases, is mutated compared to the corresponding amino acid which appears in the corresponding wild-type luciferase sequence. The recombinant luciferase has increased thermostability compared to the corresponding wild-type luciferase.
    Type: Application
    Filed: February 9, 2011
    Publication date: July 21, 2011
    Inventors: David J. Squirrell, Melenie J. Murphy, Rachel L. Price, Christopher R. Lowe, Peter J. White, Laurence C. Tisi, James A. H. Murray
  • MUTANT LUCIFERASE
    Publication number: 20140170687
    Abstract: An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata, Luciola lateralis, Hotaria parvula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia miyako or Photinus pennsylvanica. In the sequence of the recombinant luciferase, the amino acid residue corresponding to phenylalanine 295 in Photinus pyralis wild-type luciferase or to leucine 297 in Luciola mingrelica, Luciola cruciata or Luciola lateralis wild-type luciferases, is mutated compared to the corresponding amino acid which appears in the corresponding wild-type luciferase sequence. The recombinant luciferase has increased thermostability compared to the corresponding wild-type luciferase.
    Type: Application
    Filed: January 17, 2014
    Publication date: June 19, 2014
    Applicant: PROMEGA CORPORATION
    Inventors: David J. Squirrell, Melenie J. Murphy, Rachel L. Price, Christopher R. Lowe, Peter J. White, Laurence C. Tisi, James A. H. Murray
  • Mutant luciferase
    Patent number: 8652794
    Abstract: An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata, Luciola lateralis, Hotaria parvula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia miyako or Photinus pennsylvanica. In the sequence of the recombinant luciferase, the amino acid residue corresponding to phenylalanine 295 in Photinus pyralis wild-type luciferase or to leucine 297 in Luciola mingrelica, Luciola cruciata or Luciola lateralis wild-type luciferases, is mutated compared to the corresponding amino acid which appears in the corresponding wild-type luciferase sequence. The recombinant luciferase has increased thermostability compared to the corresponding wild-type luciferase.
    Type: Grant
    Filed: February 9, 2011
    Date of Patent: February 18, 2014
    Assignee: Promega Corporation
    Inventors: David J. Squirrell, Melenie J. Murphy, Rachel L. Price, Christopher R. Lowe, Peter J. White, Laurence C. Tisi, James A. H. Murray
  • Mutant luciferase of a firefly, mutant luciferase genes, recombinant DNAS containing the genes and a method of producing mutant luciferase
    Patent number: 5219737
    Abstract: The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.
    Type: Grant
    Filed: March 26, 1991
    Date of Patent: June 15, 1993
    Assignee: Kikkoman Corporation
    Inventors: Naoki Kajiyama, Eiichi Nakano
  • Modified Luciferases And Uses Thereof
    Publication number: 20170335293
    Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases with different emission spectra.
    Type: Application
    Filed: June 29, 2017
    Publication date: November 23, 2017
    Inventor: Rampyari Walia
  • Modified Luciferases And Uses Thereof
    Publication number: 20160264944
    Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases with different emission spectra.
    Type: Application
    Filed: January 20, 2016
    Publication date: September 15, 2016
    Inventor: Rampyari Walia
  • FIREFLY LUCIFERASE, ITS GENE, AND METHOD FOR PRODUCING FIREFLY LUCIFERASE
    Publication number: 20120295325
    Abstract: A firefly luciferase having the amino acid sequence of firefly luciferase, wherein the amino acid corresponding to position 287 of Heike firefly luciferase is replaced with alanine, or wherein the amino acid corresponding to position 392 is replaced with isoleucine. The firefly luciferase has improved thermostability and storage stability. A firefly luciferase gene coding for the firefly luciferase. By utilizing this gene, it is possible to efficiently produce firefly luciferase with increased stability. It is also possible to obtain firefly luciferase with further increased stability by combination with a mutation in which the amino acid at position 326 is replaced with serine, and/or a mutation in which the amino acid at position 467 is replaced with isoleucine.
    Type: Application
    Filed: February 16, 2010
    Publication date: November 22, 2012
    Applicant: KIKKOMAN CORPORATION
    Inventors: Yukako Kodama, Eriko Yoshihara
  • Multiplex assays with multiple luciferases reporters and uses thereof
    Patent number: 9353401
    Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases reporters with different emission spectra and different substrates for simultaneous luciferase measurements.
    Type: Grant
    Filed: August 27, 2010
    Date of Patent: May 31, 2016
    Assignee: Targeting Systems
    Inventor: Rampyari Walia
  • Covalent modification and conjugation of luciferase
    Publication number: 20070254311
    Abstract: A process for reversible chemical modification of the luciferase, a process for the covalent conjugation of a reversibly modified luciferase to a chemical moiety (a protein or a binding partner such as biotin or an antibody), a process for reactivation of the reversibly-modified and inactivated luciferase, a process for making the said luciferase conjugates and a bioluminescent assay method that uses covalently conjugated firefly luciferase are taught. The present invention also relates to a composition comprising a reversibly modified luciferase, as well as a composition comprising a reversibly modified luciferase covalently conjugated to a chemical moiety.
    Type: Application
    Filed: April 26, 2006
    Publication date: November 1, 2007
    Applicant: CardioGenics Inc.
    Inventors: Amer Alagic, Pavel Zhelev, Yahia Gawad
  • Modified luciferases and uses thereof
    Patent number: 9732328
    Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases with different emission spectra.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: August 15, 2017
    Assignee: Targeting Systems
    Inventor: Rampyari Walia
  • LUCIFERASES AND USES THEREOF
    Publication number: 20120156705
    Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases with different emission spectra.
    Type: Application
    Filed: August 27, 2010
    Publication date: June 21, 2012
    Applicant: Targeting Systems
    Inventor: Rampyari Walia
  • Method and compositions for detecting luciferase biological samples
    Patent number: 6171809
    Abstract: The presence of renilla luciferase alone or both renilla luciferase and firefly luciferase is detected by adding reagent mixture(s) to a biological sample and producing glow luminescence having a duration of at least an hour. In producing luminescence from renilla luciferase alone, a reagent is added comprising coelenterazine, and dithiothreitol (DTT) and EDTA, or functional equivalents of DTT and EDTA. Luminescence from samples containing both firefly luciferase and renilla luciferase is produced by first adding a reagent comprising firefly luciferin, ATP, co-factors necessary for firefly luciferase activity (e.g., Ca+2 and Mg+2), dithiothreitol (DTT) or functional equivalents thereof, and AMP. Following measurement of the firefly luciferase, coelenterazine and EDTA, or functional equivalents of EDTA, are added, and the luminescence produced by renilla luciferase is measured.
    Type: Grant
    Filed: January 29, 1998
    Date of Patent: January 9, 2001
    Assignee: Packard Instrument Company
    Inventor: Christiaan Roelant
  • BIOLUMINESCENT ASSAYS UTILISING SECRETED LUCIFERASES
    Publication number: 20130217593
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Application
    Filed: March 12, 2013
    Publication date: August 22, 2013
    Inventors: Marco Peter Leu, John Michael Daly
  • BIOLUMINESCENT ASSAYS UTILISING SECRETED LUCIFERASES
    Publication number: 20150191769
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Application
    Filed: March 17, 2015
    Publication date: July 9, 2015
    Inventors: Marco Peter Leu, John Michael Daly
  • Bioluminescent assays utilising secreted luciferases
    Patent number: 9012164
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: April 21, 2015
    Inventors: Marco Peter Leu, John Michael Daly
  • BIOLUMINESCENT ASSAYS UTILISING SECRETED LUCIFERASES
    Publication number: 20200024637
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Application
    Filed: June 13, 2019
    Publication date: January 23, 2020
    Inventors: Marco Peter Leu, John Michael Daly
  • BIOLUMINESCENT ASSAYS UTILISING SECRETED LUCIFERASES
    Publication number: 20100092967
    Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.
    Type: Application
    Filed: December 21, 2007
    Publication date: April 15, 2010
    Applicant: GENE STREAM PTY LTD
    Inventors: Marco Peter Leu, John Michael Daly
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