Search Patents
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Publication number: 20030068801Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lifes of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.Type: ApplicationFiled: April 19, 2001Publication date: April 10, 2003Applicant: Promega CorporationInventors: Keith V. Wood, Mary P. Hall
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Patent number: 7241584Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.Type: GrantFiled: February 28, 2003Date of Patent: July 10, 2007Assignee: Promega CorporationInventors: Keith V. Wood, Mary P. Hall, Monika G. Wood
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Patent number: 7183092Abstract: The invention comprises modified luciferase proteins which are more resistant to inhibition by test chemicals than wild type luciferase. The modified luciferases also contain greater thermostability than wild type luciferase.Type: GrantFiled: September 30, 2004Date of Patent: February 27, 2007Assignee: Board of Control of Michigan Technological UniversityInventors: Eileen H. Kim Choi, David R. Shonnard, Irina V. Rybina
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Publication number: 20100281552Abstract: A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.Type: ApplicationFiled: May 3, 2010Publication date: November 4, 2010Inventors: Lance P. Encell, Keith V. Wood, Monika G. Wood, Mary Hall, Paul Otto, Gediminas Vidugiris, Kristopher Zimmerman
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Publication number: 20140186918Abstract: A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid, and preferably an uncharged polar amino acid such as tyrosine. Mutant luciferases in accordance with the invention can produce a large (50 nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. These properties make the mutant particularly useful in a variety of assays.Type: ApplicationFiled: February 14, 2014Publication date: July 3, 2014Applicant: PROMEGA CORPORATIONInventors: David James Squirrell, Melenie Jane Murphy, Rachel Louise Price, Peter John White, Tara Louise Willey
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Patent number: 8669087Abstract: A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in Photinus pyralis luciferase is mutated as compared to the corresponding wild-type luciferase, such that the luciferase enzyme is able to emit light at a different wavelength as compared to the corresponding wild-type luciferase and/or has enhanced thermostability as compared to the corresponding wild-type luciferase. In general, the residue corresponding to 357 in Photinus pyralis luciferase is changed from an acidic amino acid to a non-acidic amino acid and preferably an uncharged polar amino acid such as tyrosine. Mutant luciferases in accordance with the invention can produce a large (50 nm) wavelength shift in emitted light and have good thermostability. The resultant colour shift can be reversed by addition of coenzyme A. These properties make the mutant particularly useful in a variety of assays.Type: GrantFiled: October 26, 2000Date of Patent: March 11, 2014Assignee: Promega CorporationInventors: David James Squirrell, Melenie Jane Murphy, Rachel Louise Price, Peter John White, Tara Louise Willey
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Publication number: 20080090263Abstract: The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.Type: ApplicationFiled: October 13, 2006Publication date: April 17, 2008Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Yoshihiro Ohmiya, Chun Wu
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Patent number: 6132983Abstract: Proteins are provided having luciferase activity with greater heat stability than wildtype luciferases by replacing the glutamate equivalent to that at position 354 of Photinus pyralis luciferase or 356 of Luciola luciferases with an alternative amino acid, particularly lysine. DNA, vectors and cells that encode for and express the proteins are also provided as are test kits and reagents for carrying out luminescence assays using the proteins of the invention. Preferred proteins have a second replaced amino acid at a position equivalent to position 215 of Photinus pyralis luciferase or 217 of Luciola luciferases.Type: GrantFiled: November 20, 1996Date of Patent: October 17, 2000Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern IrelandInventors: Christopher Robin Lowe, Peter John White, James Augusts Henry Murray, David James Squirrell
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Patent number: 7871803Abstract: The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.Type: GrantFiled: December 9, 2004Date of Patent: January 18, 2011Assignee: NEC Soft, Ltd.Inventor: Hiromi Takenaka
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Publication number: 20110039257Abstract: A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.Type: ApplicationFiled: April 2, 2007Publication date: February 17, 2011Applicant: Promega CorporationInventors: Brock Binkowski, Frank Fan, Susan Wigdal, Keith V. Wood, Monika G. Wood
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Publication number: 20030135871Abstract: Native and modified forms of Phrixothrix hirtus red luciferase are described. These native and modified forms of luciferase can be used, for example, as reporter molecules in host cells and/or transgenic animals.Type: ApplicationFiled: August 15, 2002Publication date: July 17, 2003Inventors: Kevin A. Nawotka, Weisheng Zhang
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Publication number: 20020081644Abstract: A polynucleotide encoding a secreted form of wild type Renilla luciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.Type: ApplicationFiled: March 14, 2001Publication date: June 27, 2002Inventors: Alan P. Escher, Jingxue Liu
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Publication number: 20120035077Abstract: This invention provides modified nucleotide sequences encoding luciferase that have greater expression than wild type luciferase.Type: ApplicationFiled: May 27, 2011Publication date: February 9, 2012Applicant: SwitchGear GenomicsInventors: Nathan D. Trinklein, Shelley Force Aldred
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Publication number: 20090305353Abstract: The present invention provides a mutant firefly luciferase consisting of a mutant amino acid sequence derived from the amino acid sequence of a wild-type firefly luciferase by at least substitution (a), (b), or (c) below and having luminescence intensity higher than that of the wild-type firefly luciferase.Type: ApplicationFiled: September 6, 2006Publication date: December 10, 2009Inventors: Hiroya Fujii, Kenichi Noda
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Publication number: 20040235077Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.Type: ApplicationFiled: April 22, 2004Publication date: November 25, 2004Applicant: Kikkoman CorporationInventors: Noriaki Hattori, Seiji Murakami
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Publication number: 20020102687Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.Type: ApplicationFiled: April 26, 2001Publication date: August 1, 2002Applicant: CHISSO CORPORATIONInventor: Satoshi Inouye
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Publication number: 20040002127Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.Type: ApplicationFiled: March 4, 2003Publication date: January 1, 2004Applicant: CHISSO CORPORATIONInventor: Satoshi Inouye
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Patent number: 6495355Abstract: Nucleic acid compositions and polypeptides encoding a red-shifted form of firefly luciferase are provided. These red-shifted luciferases are characterized by spectrum of light emission having detectable emissions at 610 nm (luc610), preferably a primary peak at 610 nm. The nucleic acid compositions find use in various systems as a reporter gene, and are of particular interest for use as a reporter with in vivo systems, because of the efficient transfer of red light through tissues. The red-shifted luciferase may be combined in such assays with luciferases emitting at other spectra, in order to monitor multiple processes simultaneously.Type: GrantFiled: June 21, 2000Date of Patent: December 17, 2002Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Christopher Contag, Brian Eames
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Patent number: 6451549Abstract: A polynucleotide encoding a secreted form of wild type Renilla luciferase. Also provided is a polynucleotide encoding a secreted modified form of wild type Renilla luciferase. Additionally, the polypeptides encoded by the polynucleotides of the present invention and uses of the polynucleotides and polypeptides of the present invention in biological assays. Also, a stable mammalian packaging cell line which produces retroviruses carrying a polynucleotide encoding a secreted Renilla luciferase.Type: GrantFiled: March 14, 2001Date of Patent: September 17, 2002Assignee: Loma Linda UniversityInventors: Alan P. Escher, Jingxue Liu
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Patent number: 6812012Abstract: The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.Type: GrantFiled: June 26, 2000Date of Patent: November 2, 2004Assignee: Kikkoman CorporationInventors: Noriaki Hattori, Seiji Murakami