Search Patents
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Patent number: 11078519Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.Type: GrantFiled: June 13, 2019Date of Patent: August 3, 2021Assignee: GENE STREAM PTY LTD.Inventors: Marco Peter Leu, John Michael Daly
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Publication number: 20100093029Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.Type: ApplicationFiled: October 10, 2008Publication date: April 15, 2010Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
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Patent number: 8206961Abstract: A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.Type: GrantFiled: May 24, 2010Date of Patent: June 26, 2012Assignee: Marker Gene Technologies, Inc.Inventors: Daniel J. Coleman, John J. Naleway, Gabriele M. Cook
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Patent number: 8227572Abstract: Nucleotide and amino acid sequences of luciferase peptides that are encoded by genes within the genome of Arachnocampa (Diptera) are disclosed. Specifically provided are functional ATP-dependent luciferases that catalyze luminescence reactions with emission spectra within the blue portion of the spectrum. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and active subsequences of the enzyme peptides, and methods of identifying modulators and substrates of the luciferase peptides. Methods of assays, including multiple reporter assays utilizing at least two ATP-dependent luciferases are provided.Type: GrantFiled: August 18, 2006Date of Patent: July 24, 2012Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Virginia Leitch, Mira Maria Dumancic, Stephen Charles Trowell, Helen Dacres
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Patent number: 5976796Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.Type: GrantFiled: December 23, 1996Date of Patent: November 2, 1999Assignee: Loma Linda UniversityInventors: Aladar A. Szalay, Gefu Wang, Yubao Wang
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Patent number: 6544754Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector. Further, the invention provides a method for producing the recombinant Oplophorus luciferase or the photoprotein. These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.Type: GrantFiled: April 26, 2001Date of Patent: April 8, 2003Assignee: Chisso CorporationInventor: Satoshi Inouye
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Patent number: 8236540Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.Type: GrantFiled: October 22, 2007Date of Patent: August 7, 2012Assignee: Bayer Intellectual Property GmbHInventors: Stefan Golz, Bernd Kalthof, Svetlana Markova, Ludmila Frank, Eugene Vysotski
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Patent number: 5196524Abstract: Novel fusion reporter genes, fusion reporter proteins, and an improved reporter system for measuring the relative activity of a promoter sequence. A luxAB fusion gene of the present invention is particularly useful as a reporter gene and is derived from the fusion of a luxA gene and a luxB gene from Vibrio harveyi. The gene products of the luxA and luxB genes are the .alpha.- and .beta.-subunits, respectively, of a bacterial luciferase. A fusion protein encoded by a luxAB fusion gene is a single active protein and is particularly useful as a reporter protein having luciferase activity. An advantage of such a reporter system to assay gene expression in many cells which contain FMNH.sub.2, such as bacterial and yeast cells, is that an immediate and quantitative assessment of gene expression may be made from real-time light measurements using intact cells.Type: GrantFiled: January 6, 1989Date of Patent: March 23, 1993Assignee: Eli Lilly and CompanyInventors: Gary D. Gustafson, Thomas D. Ingolia, Gretchen Kirchner, Jean L. Roberts
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Patent number: 7989190Abstract: The present invention provides a firefly luciferase for inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having low reactivity to DNA polymerase in the manner of dATP?S, a method of analyzing nucleic acid that uses that luciferase, and a kit for analyzing nucleic acid thereof. The present invention relates to a composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP, a method of analyzing nucleic acid that comprises the use of that composition, and a kit for analyzing nucleic acid comprising that composition.Type: GrantFiled: November 19, 2008Date of Patent: August 2, 2011Assignee: Kikkoman CorporationInventors: Shigeya Suzuki, Yukako Kodama, Keiko Kurosawa
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Publication number: 20130005666Abstract: The NF-E2-related factor 2 (Nrf2) is a key transcriptional regulator of antioxidant defense and detoxification. To directly monitor stabilization of Nrf2 we fused its Neh2 domain, responsible for the interaction with its nucleocytoplasmic regulator, Keap1, to firefly luciferase (Neh2-luciferase). It is shown herein that Neh2 domain is sufficient for recognition, ubiquitination and proteasomal degradation of Neh2-luciferase fusion protein. The novel Neh2-luc reporter system allows direct monitoring of the adaptive response to redox stress and classification of drugs based on the time-course of reporter activation. The novel reporter was used to screen a library of compounds to identify activators of Nrf2. The most robust and yet non toxic Nrf2 activators found—nordihydroguaiaretic acid, fisetin, and gedunin-induced astrocyte-dependent neuroprotection from oxidative stress via an Nrf2-dependent mechanism.Type: ApplicationFiled: June 29, 2012Publication date: January 3, 2013Applicant: CORNELL UNIVERSITYInventors: Rajiv RATAN, Irina GAZARYAN, Natalya A. SMIRNOVA
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Publication number: 20150051373Abstract: The main object of the present invention is to provide a novel technique using a BAF. The invention that achieves the object provides the following: a chimeric protein comprising a luminescent domain and a cellulose- and/or chitin-binding domain, the luminescent domain comprising at least one luminescent protein selected from the group consisting of luciferases and fluorescent proteins.Type: ApplicationFiled: November 21, 2012Publication date: February 19, 2015Inventors: Hideto Hoshino, Koichi Uegaki
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Publication number: 20030092098Abstract: Isolated and purified nucleic acids encoding green fluorescent proteins from Renilla reniformis and the green fluorescent protein encoded thereby are also provided. Mutants of the nucleic acid molecules and the modified encoded proteins are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.Type: ApplicationFiled: March 15, 2001Publication date: May 15, 2003Inventors: Bruce Bryan, Christopher Szent-Gyorgyi, William Szczepaniak
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Publication number: 20130273111Abstract: The polynucleotide encoding the antigen Wb123 from the filarial nematode Wuchereria bancrofti, the major causative organism of lymphatic filariasis is provided, along with the polypeptide encoded by the polynucleotide. Methods for making the WM23 antigen, recombinant vectors encoding the Wb123 polynucleotide, and methods of detection of the Wb123 antigen through luciferase immunprecipitation, ELISA and other detection systems are also provided.Type: ApplicationFiled: October 31, 2011Publication date: October 17, 2013Applicant: The United States of America, as represented by the Secretary, Department of Health and Human ServInventors: Thomas B. Nutman, Doran Fink, Joseph Kubofcik, Peter D. Burbelo
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Patent number: 8926981Abstract: The present invention provides methods and compositions useful in the diagnosis and management of autoimmune diseases. In particular, the present invention provides improved methods and compositions for the diagnosis and management of Graves' disease. The methods of the present invention not only avoids the need for radioactivity and are much simpler, economical, and rapid than methods traditionally used for the diagnosis of Graves' disease, but also improve upon the sensitivity and detection abilities of previous luciferase-based autoantibody detection assays.Type: GrantFiled: September 12, 2012Date of Patent: January 6, 2015Assignee: Diagnostic Hybrids, Inc.Inventor: James L. Brown
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Publication number: 20120190824Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: ApplicationFiled: May 28, 2010Publication date: July 26, 2012Applicants: THE UNIVERSITY OF TOKYO, TOYO BOSEKI KABUSHIKI KAISHA, PROBEX INC.Inventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Patent number: 6670449Abstract: The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.Type: GrantFiled: February 13, 1998Date of Patent: December 30, 2003Assignee: Memorial Sloan-Kettering Cancer CenterInventors: Gero Miesenböck, Dino De Angelis, James E. Rothman
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Publication number: 20040137611Abstract: The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.Type: ApplicationFiled: September 30, 2003Publication date: July 15, 2004Inventors: Gero Miesenbock, Dino De Angelis, James E. Rothman
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Patent number: 7094888Abstract: The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.Type: GrantFiled: September 30, 2003Date of Patent: August 22, 2006Assignee: Memorial Sloan-Kettering Cancer CenterInventors: Gero Miesenböck, Dino De Angelis, James E. Rothman
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Patent number: 8470974Abstract: The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.Type: GrantFiled: May 28, 2010Date of Patent: June 25, 2013Assignees: The University of Tokyo, ProbeX Inc., Tokyo Boseki Kabushiki KaishaInventors: Takeaki Ozawa, Naomi Misawa, Kenji Miura, Tasuku Okamoto, Shigeaki Nishii, Kenji Masuda
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Patent number: 8124424Abstract: The claimed invention comprises a single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, wherein the ligand-binding molecule comprises a ligand-binding domain (LBD) of a nuclear receptor and an LBD-interacting domain that is a co-activator peptide of said nuclear receptor, and an N-terminal polypeptide and a C-terminal polypeptide of a click beetle luciferase (N-CBLuc and C-CBLuc), which flank each end of the ligand-binding molecule, respectively, wherein the N-CBLuc and the C-CBLuc self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule.Type: GrantFiled: January 15, 2008Date of Patent: February 28, 2012Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Yoshio Umezawa, Moritoshi Sato, Hiroaki Tao, SungBae Kim