Abstract: A microbial decontamination system includes a chamber for housing items to be decontaminated as well as a source of one or more iodine-containing fluids that are conveyed to the chamber as necessary. The system also includes a detector element for monitoring the concentration of free iodine within the chamber. A signal representative of the free iodine concentration is conveyed to a controller element which communicates with receptacles for housing the iodine-containing fluids so that additional iodine-containing fluids may be added to maintain the free iodine concentration within the chamber at a desired level.