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  • Fluorescent proteins, their production and use
    Patent number: 8536314
    Abstract: The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.
    Type: Grant
    Filed: May 19, 2010
    Date of Patent: September 17, 2013
    Assignee: Evocatal GmbH
    Inventors: Thorsten Eggert, Sascha Hausmann, Michael Puls