Abstract: This invention relates to an novel Gluconobacter oxydans 2-ketoreductase useful for the synthesis of chiral alcohols. Also related are isolated nucleic acids encoding G. oxydans 2-ketoreductase enzymes, and enzyme fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated G. oxydans 2-ketoreductase polypeptides, and fragments and variants thereof, and antibodies that specifically bind to G. oxydans 2-ketoreductase polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated G. oxydans 2-ketoreductase nucleic acids, polypeptides, and antibodies, and methods of using G. oxydans 2-ketoreductase in various reactions for industrial or pharmaceutical applications.
Type:
Application
Filed:
December 16, 2002
Publication date:
February 5, 2004
Inventors:
Venkata B. Nanduri, Robert M. Johnston, Steven L. Goldberg, Paul M. Cino, Ramesh N. Patel
Abstract: A method for the production of taxanes such as taxol. The method includes inducing formation of callus cells by contacting an explant tissue with a liquid medium without complete submergence of the tissue in the medium. The callus cells formed are employed in a liquid suspension cell culture to produce one or more taxanes.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
September 9, 1997
Assignee:
E. R. Squibb & Sons, Inc.
Inventors:
Paul M. Cino, Steven R. Schwarz, Dana L. Cazzulino
Abstract: An enzymatic hydroxylation process for the preparation of compounds useful as HMG-CoA reductase inhibitors and/or as intermediates in the preparation of HMG-CoA reductase inhibitors uses a microorganism or an enzyme derived from, or having the structure of an enzyme derived from, said microorganism, which is capable of catalyzing the hydroxylation process.
Type:
Grant
Filed:
October 22, 1993
Date of Patent:
March 28, 2000
Assignee:
Bristol-Myers Squibb Company
Inventors:
Brian L. Davis, Paul M. Cino, Laszlo Szarka