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  • Method for Cellular RNA Expression
    Publication number: 20140030808
    Abstract: The present invention relates to enhancing RNA expression in a cell such as a cell transfected with RNA by reducing the activity of RNA-dependent protein kinase (PKR). Thus, the present invention provides methods for expressing RNA in a cell comprising the step of reducing the activity of RNA-dependent protein kinase (PKR) in the cell. Reducing the activity of RNA-dependent protein kinase (PKR) in the cell increases the stability of RNA and/or increases the expression of RNA in the cell.
    Type: Application
    Filed: December 2, 2011
    Publication date: January 30, 2014
    Applicants: BIONTECH AG, Universitatsmedizin der Johannes Gutenberg- Universitat Mainz, Translationale Onkologie an der Universitatsmediz- in der JGU Mainz gGmbH (TRON)
    Inventors: Ugur Sahin, Tim Beissert, Marco Poleganov, Stephanie Herz
  • Antigen-specific clonal expansion of B cells
    Patent number: 8945923
    Abstract: The present invention provides recombinant proteins comprising the amino acid sequence of an intracellular segment of CD40 and an amino acid sequence mediating the association of the recombinant protein with the constant region of an immunoglobulin heavy chain. The recombinant proteins according to the present invention are useful for inducing clonal expansion of a B cell having a predetermined antigen-specificity without the need for T cell or CD40L mediated co-stimulation. Thus, the present invention provides tools for clonal expansion of B cells specific for an antigen of interest and the production of B cells secreting antibodies specific for an antigen of interest. The recombinant proteins of the present invention may also be used for generating fully human monoclonal antibodies with a predetermined antigen-specificity from the B cell repertoire of a human subject.
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: February 3, 2015
    Assignees: BioNTech AG, Universitatsmedizin der Johannes Gutenberg-Universitat
    Inventors: Ugur Sahin, Ozlem Tureci, Torsten Seppmann, Holger Hoff, Jens Schumacher
  • Polynucleotides encoding an intracellular segment of CD40 and a constant region of an immunoglobulin heavy chain
    Patent number: 9139639
    Abstract: The present invention provides recombinant proteins comprising the amino acid sequence of an intracellular segment of CD40 and an amino acid sequence mediating the association of the recombinant protein with the constant region of an immunoglobulin heavy chain. The recombinant proteins according to the present invention are—useful for inducing clonal expansion of a B cell having a predetermined antigen-specificity without the need for T cell or CD40L mediated co-stimulation. Thus, the present invention provides tools for clonal expansion of B cells specific for an antigen of interest and the production of B cells secreting antibodies specific for an antigen of interest. The recombinant proteins of the present invention may also be used for generating fully human monoclonal antibodies with a predetermined antigen-specificity from the B cell repertoire of a human subject.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: September 22, 2015
    Assignees: BioNTech AG, Universitatsmedizin Der Johannes Gutenberg-Universitat Mainz
    Inventors: Ugur Sahin, Ozlem Tureci, Torsten Seppmann, Holger Hoff, Jens Schumacher