Abstract: Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in high throughput methods and can be multiplexed.

Abstract: A microplate for use within an interrogation system and a method of using the microplate are disclosed. The microplate contains within the bottom of each well, an optical waveguide grating based sensor. Approximate to each sensor is a mask having an aperture of predetermined size. The aperture regulates the light that enters and exits the sensor upon successive scans and ensures repeatable readings from the sensor. In an extended embodiment, a method of detection is disclosed that utilizes a launch and receive system while employing the aforementioned microplate.

Abstract: A composite including interpenetrating networks of an organic polymer, such as from an acrylate or olefin, having urethane groups; and an inorganic polymer, the composite having a low or no-shrinkage characteristic, and a method for making the composite, as defined herein.

Abstract: A swept wavelength interrogation system includes a tunable light source for outputting a light beam that is tunable over a range of wavelengths and an optical reader head for distributing the light beam among a plurality of sensors and for measuring response spectra from the sensors. A wavelength-tracking device measures centroid wavelengths of the light beam. A processor calculates a centroid wavelength of the response spectra from the sensors based on the measured centroid wavelengths of the light beam.

Abstract: A functional assay detection system for membrane bound proteins. The system comprises a biological array including a porous substrate having a plurality of membranes adhered thereto and a first side and a second side, a fluorescent labeling reagent configured to couple to the membrane bound proteins, a pulsed light assembly configured to excite the fluorescent labeling reagent, and a time-delayed imaging device configured to capture emitted fluorescence of the fluorescent labeling reagent. The pulsed light assembly is configured to excite the fluorescent labeling reagent from at least one of the first side and the second side of the porous substrate, and the fluorescent labeling reagent comprises a fluorophore that has an emission lifetime that is in the range of microseconds.

Abstract: Described herein are supports for assaying an analyte and methods of making and using thereof.

Abstract: A system and a method as defined herein for scan interrogation of, for example, a label-independent-detection (LID) biosensor, such as for monitoring a surface change or an event on a biosensor for use, for example, in microplate image analysis.

Abstract: The disclosure provides a composition including a porous cordierite honeycomb body as defined herein having excellent strength, high thermal shock resistance, and reduced microcrack properties. The disclosure also provides methods of making and using the composition, for example, as a catalyst carrier.

Abstract: Methods for producing optical fibers along nonlinear paths include incorporating fluid bearings. An optical fiber is drawn from a preform along a first pathway, contacted with a region of fluid cushion of a fluid bearing, and redirected along a second pathway as the fiber is drawn across said region of fluid cushion.

Abstract: A nanocomposite material including organic-inorganic polymeric interpenetrating networks having a low or no-shrinkage characteristic and a method for making the composite, as defined herein.

Abstract: Disclosed is a cell culture article including: a substrate; a tie-layer attached to at least the substrate; and a bio-compatible layer attached to at least the tie layer, the bio-compatible layer having been obtained from surface oxidation of a polymer layer. Also disclosed are methods for making the cell culture article and methods for performing an assay of a ligand with the article.

Abstract: Fused thiophene (FT) compounds, FT polymers, FT containing articles, and methods for making and using the FT compounds and polymers thereof of the formulas, as defined herein.

Abstract: A fluorescent ion doped glass (or other fluorescing material) and a method for using the fluorescent ion doped glass to enhance a fluorescence imaging technique are described herein. In one embodiment, the fluorescent ion doped glass (or other fluorescing material) is used as a calibration standard to check the uniformity of an intensity profile of an excitation source. In another embodiment, the fluorescent ion doped glass (or other fluorescing material) is used as an offline calibration standard to normalize a native fluorescence image of one or more test samples (e.g., a protein array). In yet another embodiment, the fluorescent ion doped glass (or other fluorescing material) is used as an online calibration standard to normalize a native fluorescence image of one or more test samples (e.g., a protein array).

Abstract: A wavelength tunable system including: a laser having a lasing cavity and an external cavity; a frequency-adjuster in the external cavity; and an adjustable current source to adjust the laser diode current, and a method of use as defined herein.

Abstract: Disclosed are ceramic batch compositions for forming porous ceramic articles. The ceramic forming precursor batch compositions include ceramic forming inorganic batch components and a cyclododecane pore forming agent. Also disclosed are methods for manufacturing porous ceramic articles.

Abstract: A method for passivating a porous ceramic article containing microcracks including filling the microcracks with a liquid, such as water, and then applying a washcoat as described herein.

Abstract: A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

Abstract: Labels, methods of making labels and methods of using labels are disclosed. The labels can be manufactured using fiber drawing techniques or by shutter masking. The labels can be used for detecting the presence of an analyte in a sample and for detecting interactions of biomolecules.

Abstract: The disclosure relates to a system and a method for light beam interrogation of an optical biosensor and for monitoring a biological event on the biosensor for use, for example, in microplate image analysis. The system and method correct pointing-errors that can be encountered, for example, in scanning label-independent-detection biosensor applications.

Abstract: Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5?-diphosphate (GDP) salt, and/or an anti-oxidant reagent.