Abstract: A process for expression of a protein product in Aspergillus oryzae is disclosed. The process comprises transforming Aspergillus oryzae with a vector system comprising DNA-sequences encoding functions facilitating gene expression, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in A. oryzae. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus. A preferred promoter is the TAKA-amylase promoter or functional parts thereof. There is also provided a process for the production of a recombinant Humicola lipase. The recombinant Humicola lipase from A. oryzae differs from the native lipase in having a greater glycosylation and in exhibiting an improved thermostability.

Abstract: The present invention relates to isolated nucleic acid fragments containing a sequence encoding a Rhizoctonia solani laccase having optimum activity at a neutral or basic pH. and the laccase proteins encoded thereby.

Abstract: A protease obtainable from Dendryphiella arenaria DSM 6260 or Dendryphiella salina DSM 6332 is disclosed. The protease has a pl of approximately 9.1 as determined by isoelectric focusing, a molecular weight of approximately 18-20 kD as determined by SDS-PAGE, is active towards haemoglobin, casein, skimmed milk, and Suc-Ala-Ala-Pro-Phe-pNA and has immunochemical properties identical to those of a protease obtained from Dendryphiella arenaria DSM 6260 or Dendryphiella salina DSM 6332. A process for its production, use in a detergent composition and as a detergent additive are disclosed.

Abstract: A xylanase obtained from Rhodothermus marinus, the method of obtaining it and a process for its use are disclosed. The xylanase has an optimum temperature of from 85.degree. to 100.degree. C., a relative activity of more than 50% in the interval of from pH 5 tp pH 8 after incubation for 5 minutes at 65.degree. C., a relative temperature stability at 80.degree. C. of more than 80% after incubation at pH 7 for 3 hours, a pH optimum of about 6 and an isoelectric point in the range of from 3 to 7. The xylanase can be obtained from the strains ATCC 43812, ATCC 43813 or a mutant thereof. The process for obtaining the xylanase involves cultivation of a xylanase-producing strain of Rhodothermus marinus in a suitable nutrient medium containing carbon and nitrogen sources and inorganic salts followed by recovery of the xylanase. Also disclosed is a process for the treatment of lignocellulosic pulp with the xylanase.

Abstract: An enzyme containing T-granulate, coated with a coating agent comprising a high melting fat or wax, is combined with a mixture containing fodder components, and the combination is steam treated and subsequently pelletized. The method considerably improves the stability of the enzyme in the pelletized mixture.

Abstract: Insulin preparations containing nicotinamide or nicotinic acid or a salt thereof shown interesting pharmacological properties.

Abstract: A method for making TFPI analogues lacking part of the C-terminal end of the native TFPI molecule is described by cultivation of a yeast strain transformed with an expression vector containing a DNA sequence encoding such TFPI analogues. The TFPI analogues will at least contain the two first Kunitz domains and lack the third Kunitz domain.

Abstract: A process for the microbial production of a protein susceptible to inactivation in a fluid production medium by continuously and reversibly protecting said protein against said inactivation during the production stage, separating the protein from the production medium, deprotecting the protein, and recovering the protein product. The process is especially useful for obtaining substantially increased yields of the protein in question by continuously precipitating said protein.

Abstract: This invention is in the field of detergent proteases derived from strains of a new Bacillus sp. More specifically, the invention is directed towards a protease derived from a strain of Bacillus sp. TY 145. Moreover, the invention is directed towards a process for the preparation of the protease the use of the protease enzyme, and detergent composition comprising the protease of the invention.

Abstract: An alkaline protease isolated from a strain of Bacillus sp. J 20 is disclosed. The protease has a mass of about 29 kD, a pI of about 8.8, a pH optimum above 10, and a temperature optimum of about 45.degree. to 65.degree. C. The use of the protease as a detergent enzyme, as well as detergent compositions and detergent additives comprising the protease is also disclosed.

Abstract: In a method of recovering proteins, an antibody raised against a peptide comprising at least a fragment of the protein is immobilized on a solid support, a crude preparation of the protein is contacted with the antibody under conditions resulting in a partial, reversible unfolding of the protein to obtain binding of the protein to the antibody, and the protein is eluted from the solid support under non-denaturing conditions to recover the protein in a form in which it is refolded into its native conformation.

Abstract: Novel extrinsic pathway inhibitors (EPI) are provided wherein one or more of the amino acid residues of native EPI have been deleted. A preferred group of the novel EPI analogues have no or a low heparin binding capacity. The novel EPI analogues can be used for the treatment of patients having coagulation disorders or cancer.

Abstract: The ALDC derivative is produced by treating ALDC in an aqueous medium with glutaraldehyde in defined proportions with optional subsequent immobilization. The ALDC derivative which is used in beer fermentation exhibits a satisfactory stability at low pH.

Abstract: Imidazotriazoloquinazoline compounds having the general formula ##STR1## wherein A together with the .alpha.-marked carbon atom and the .beta.-marked nitrogen atom is one of the groups ##STR2## cyano or CO.sub.2 R.sup.5, wherein R.sup.5 is H, alkyl, cycloalkyl, trifluoromethyl or alkoxymethyl; and R.sup.2, R.sup.3 and R.sup.4 independently are H, hydroxy, halogen, CN, alkyl, alkenyl, alkynyl, trifluoromethyl, alkoxy, dialkylaminoalkoxy, aralkoxy, aryloxy which may be substituted, acyclic amino group, or NR.sup.6 R.sup.7, wherein R.sup.6 and R.sup.7 independently are H or alkyl.The compounds are useful in psychopharmaceutical preparations as anticonvulsants, anxiolytics, hypnotics, antipsychotics, antiemetics, or in improving the cognitive function of the brain of mammals, or as benzodiazepine antagonsists.

Abstract: The receptor:ligand complex is applied to a solid phase substrate which binds the complex by specifically binding the ligand. The receptor is dissociated from the substrate bound receptor:ligand complex with an eluant capable of releasing the receptor from the ligand and leaving the ligand bound to the solid phase substrate. GTP containing eluants are effective. The receptor is recovered from the eluate and optionally subjected to further purification.

Abstract: The invention relates to novel DNA and amino sequences for a human MK protein. Also described are expression vectors and host cells useful in a method for production of the MK protein.

Abstract: The invention relates to compositions for the parenteral administration of an essentialy uniform and continuous amount of a phenylethanolamine derivative over an extended period of time. The invention also relates to the preparation and administration of said compositions.

Abstract: The present invention relates to a nucleotide and amino acid sequence of a 30 kilodalton outer membrane protein of Bordetella pertussis. The invention also relates to host cells and vectors comprising the nucleotide sequence, as well as a vaccine composition comprising the substantially pure protein.

Abstract: The present invention relates to novel purified gibbon ape leukemia receptor proteins and purified DNA sequences encoding these receptor proteins. The invention also relates to a method for identifying receptor proteins using the isolated DNA sequence as a probe, and a method for regulating viral entry into cells by manipulation of the GALV receptor.