Patents Represented by Attorney, Agent or Law Firm Mark K. Johnson
  • Mapping method for a microscope slide
    Patent number: 6734442
    Abstract: A mapping method for producing an infra-red map utilizing IR coatings on substrates such as microscope slides. The invisible map can be used for positioning a microscope platform or for locating microscopic objects without interference from visible or ultraviolet light.
    Type: Grant
    Filed: May 5, 1998
    Date of Patent: May 11, 2004
    Inventors: Lawrence L. Hause, Dean Jeutter
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 6706922
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: November 16, 2001
    Date of Patent: March 16, 2004
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
  • Micellar systems
    Patent number: 6673612
    Abstract: A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
    Type: Grant
    Filed: February 21, 2002
    Date of Patent: January 6, 2004
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 6630351
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Grant
    Filed: June 7, 2000
    Date of Patent: October 7, 2003
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Intravascular delivery of non-viral nucleic acid
    Patent number: 6627616
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Grant
    Filed: November 23, 1999
    Date of Patent: September 30, 2003
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Method for single-step attachment of a label to target molecules
    Patent number: 6593465
    Abstract: Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Grant
    Filed: January 23, 2001
    Date of Patent: July 15, 2003
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Paul Michael Slattum, Vladimir Budker
  • Nucleic acid transfer complexes
    Patent number: 6458382
    Abstract: The present invention relates to compositions and methods for transferring nucleic acids into cells in vitro and in vivo. The compositions comprise a transfection reagent and one or more detergents. In preferred embodiments, the compositions comprise delivery systems providing nucleic acid transfer complexes that transfect cells with high efficiency.
    Type: Grant
    Filed: November 10, 2000
    Date of Patent: October 1, 2002
    Assignee: Mirus Corporation
    Inventors: Hans Herweijer, Vladimir G. Budker
  • Reverse micelles for delivery of nucleic acids
    Patent number: 6429200
    Abstract: A complex is described for delivery to a cell comprising inserting a nucleic acid into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery. Other molecules are used to interact with the nucleic acid—micelle complex to further enhance delivery such as a surfactant having a disulfide bond.
    Type: Grant
    Filed: July 16, 1999
    Date of Patent: August 6, 2002
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
  • Polyampholytes for delivering polyions to a cell
    Patent number: 6383811
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: January 2, 2001
    Date of Patent: May 7, 2002
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy
  • Intravascular delivery of non-viral nucleic acid
    Patent number: 6379966
    Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
    Type: Grant
    Filed: November 29, 1999
    Date of Patent: April 30, 2002
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 6339067
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: December 30, 1997
    Date of Patent: January 15, 2002
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
  • Process for glucuronidation screening
    Patent number: 6319678
    Abstract: A process used to identify activity of conjugative enzymes involved in xenobiotic transformations, such as glucuronosyltransferases.
    Type: Grant
    Filed: June 25, 1999
    Date of Patent: November 20, 2001
    Assignee: PanVera Corporation
    Inventors: Olga Trubetskoy, Peter M. Shaw
  • Process of delivering naked DNA into a hepatocyte via bile duct
    Patent number: 6265387
    Abstract: A process for delivering a polynucleotide to a parenchymal cell in a mammal by injecting the polynucleotide into a blood vessel connected to the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell and functionally expressed to therapeutic levels.
    Type: Grant
    Filed: November 21, 1997
    Date of Patent: July 24, 2001
    Assignee: Mirus, Inc.
    Inventors: Jon A. Wolff, Vladimir Budker, Stuart J. Knechtle
  • Single-step method for labeling nucleic acids with mustard or aziridine labeling reagents
    Patent number: 6262252
    Abstract: Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Grant
    Filed: December 2, 1997
    Date of Patent: July 17, 2001
    Assignee: Mirus, Inc.
    Inventors: Jon A. Wolff, James E. Hagstrom, Paul Michael Slattum, Vladimir Budker
  • Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein
    Patent number: 6180784
    Abstract: The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are cationic amphipathic compounds.
    Type: Grant
    Filed: January 17, 1998
    Date of Patent: January 30, 2001
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Jeffery Fritz
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 6126964
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: January 3, 1997
    Date of Patent: October 3, 2000
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
  • Shield device for a motorcycle windscreen
    Patent number: 6120083
    Abstract: A shield device for a motorcycle windscreen which reduces reflected or refracted light from an unshielded windscreen to an operator, especially at night and aids in deflecting air and airborne road material without scratching or otherwise damaging the windscreen.
    Type: Grant
    Filed: September 16, 1996
    Date of Patent: September 19, 2000
    Inventor: John Charles Gunther
  • Process for reverse transcriptase activity measurement using fluorescence polarization
    Patent number: 6100039
    Abstract: Described is a process for detecting reverse transcriptase activity and, thereby, reverse transcriptase inhibitors using fluorescence polarization, comprising, mixing a DNA primer with an RNA template. Then forming an RNA/DNA duplex utilizing the reverse transcriptase and removing the RNA from the RNA/DNA duplex to form single-stranded DNA. Finally, adding a fluorescent-labeled oligonucleotide complementary to the single-stranded DNA for hybridizing to the single-stranded DNA; and, measuring the fluorescence polarization.
    Type: Grant
    Filed: January 28, 1999
    Date of Patent: August 8, 2000
    Assignee: Panvera, Inc.
    Inventors: Thomas J. Burke, Randall E. Bolger, Francis J. Lenoch
  • Method for covalent attachment of compounds to genes
    Patent number: 6093701
    Abstract: The described invention relates to methods for covalently attaching a compound to a gene. The method provides for covalently attaching compounds to genes for enhancing the cellular transport of the genes to predetermined targets, while maintaining the gene's functionality.
    Type: Grant
    Filed: December 12, 1997
    Date of Patent: July 25, 2000
    Assignee: Mirus, Inc.
    Inventors: Jon A. Wolff, James E. Hagstrom, Magdolna G. Sebestyen, Vladimir Budker
  • Pizza slicer
    Patent number: D474081
    Type: Grant
    Filed: February 7, 2002
    Date of Patent: May 6, 2003
    Inventors: Larry D. Rather, Richard J. Daley, Charles F. Sakris, Larry E. Staley