Patents Represented by Attorney, Agent or Law Firm Norman J. Kruse
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Patent number: 7148054Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.Type: GrantFiled: July 11, 2002Date of Patent: December 12, 2006Assignee: Maxygen, Inc.Inventors: Stephen delCardayre, Matthew Tobin, Willem P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswatan Subramanian, Linda A. Castle, Claus M. Krebber, Steve Bass, Ying-Xin Zhang, Tony Cox, Gjalt Huisman, Ling Yuan, Joseph A. Affholter
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Patent number: 6831158Abstract: Polypeptide conjugates with G-CSF activity comprising a polypeptide having at least one introduced lysine residue and at least one removed lysine residue compared to the sequence of human G-CSF, and which are conjugated to 2-6 polyethylene glycol moieties. The conjugates have a low in vitro bioactivity, a long in vivo half-life, a reduced receptor-mediated clearance, and provide a more rapid stimulation of production of white blood cells and neutrophils than non-conjugated recombinant human G-CSF.Type: GrantFiled: July 10, 2002Date of Patent: December 14, 2004Assignee: Maxygen Holdings Ltd.Inventors: Torben Lauesgaard Nissen, Kim Vilbour Andersen, Christian Karsten Hansen, Jan Moller Mikkelsen, Hans Thalsgaard Schambye
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Patent number: 6806063Abstract: Conjugates of Factor VII (FVII) and Factor VIIa (FVIIA) are provided, as are methods for preparing them. Methods for producing novel polypeptides contributing to the production of such conjugates are provided. Methods of treatment by administering a FVII or FVIIa conjugate are provided.Type: GrantFiled: February 12, 2001Date of Patent: October 19, 2004Assignees: Maxygen ApS, Maxygen Holdings Ltd.Inventors: Anders Hjelholt Pedersen, Kim Vilbour Andersen, Claus Bornaes
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Patent number: 6716631Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.Type: GrantFiled: March 1, 2000Date of Patent: April 6, 2004Assignee: Maxygen, Inc.Inventors: Stephen delCardayre, Matthew Tobin, William P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten
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Patent number: 6703240Abstract: The invention provides methods for generating, identifying, and selecting polynucleotides encoding novel starch metabolizing enzymes (NSME), NSME-encoding polynucleotides, compositions of recombinant shuffled NSME protein, plant cells and microbes containing a shuffled NSME polynucleotide in expressible form, plants containing a shuffled NSME polynucleotide in expressible form, novel starch compositions produced by plants and cells, uses of such plants, cells, and starch compositions.Type: GrantFiled: April 12, 2000Date of Patent: March 9, 2004Assignee: Maxygar, Inc.Inventors: Willem P. C. Stemmer, Venkitswaran Subramanian, Sun Ai Raillard, Gjalt Huisman
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Patent number: 6686515Abstract: Methods for evolving recombinase protein homologues and RecA/VirE2 fusion proteins which complement VirE2 deficient Agrobacterium are provided. The use of recombinase protein homologues and RecA/VirE2 fusion proteins in the context of Agrobacterium mediated transformation are provided. Methods for producing transgenic organisms by homologous recombination using evolved recombinase proteins and Agrobacterium strains which express recombinase protein homologues or RecA/VirE2 fusion proteins are provided. Transgenic cells and organisms which have integrated an exogenous DNA sequence into a predetermined site in their genome are provided.Type: GrantFiled: November 22, 2000Date of Patent: February 3, 2004Assignee: Maxygen, Inc.Inventors: Michael Lassner, Steven delCardayre
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Patent number: 6653072Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.Type: GrantFiled: June 24, 1999Date of Patent: November 25, 2003Assignee: Maxygen, Inc.Inventors: Phillip A. Patten, Willem P. C. Stemmer
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Patent number: 6646110Abstract: The invention relates to polypeptide conjugates comprising a polypeptide exhibiting G-CSF activity and having an amino acid sequence that differs from the amino acid sequence of human G-CSF in at least one specified introduced and/or removed amino acid residue comprising an attachment group for a non-polypeptide moiety, and having at least one non-polypeptide moiety attached to an attachment group of the polypeptide. The attachment group may e.g., be a lysine, cysteine, aspartic acid or glutamic acid residue or a glycosylation site, and the non-polypeptide moiety may e.g., be a polymer such as polyethylene glycol or an oligosaccharide. The conjugate has one or more improved properties such as increased biological half-life and reduced side effects.Type: GrantFiled: January 10, 2001Date of Patent: November 11, 2003Assignee: Maxygen Holdings Ltd.Inventors: Torben Lauesgaard Nissen, Kim Vilbour Andersen, Christian Karsten Hansen, Jan Moller Mikkelsen, Hans Thalsgard Schambye
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Patent number: 6613514Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.Type: GrantFiled: April 27, 2000Date of Patent: September 2, 2003Assignee: Maxygen, Inc.Inventors: Phillip A. Patten, Willem P. C. Stemmer
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Patent number: 6605430Abstract: This invention provides improved monoxygenases, dehydrogenases, and transferases that are useful for the biocatalytic synthesis of compounds such as &agr;-hydroxycarboxylic acids, and aryl- and alkyl-hydroxy compounds. The polypeptides provided herein are improved in properties such as regioselectivity, enzymatic activity, stereospecificity, and the like. Methods for obtaining recombinant polynucleotides that encode these improved polypeptides are also provided, as are organisms that express the polypeptides and are thus useful for carrying out said biocatalytic syntheses. Also provided by the invention are methods for increasing said solvent resistance of organisms that are used in the synthetic methods.Type: GrantFiled: August 12, 1999Date of Patent: August 12, 2003Assignee: Maxygen, Inc.Inventors: Joseph A. Affholter, S. Christopher Davis, Sergey A. Selifonov
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Patent number: 6602986Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: November 15, 2000Date of Patent: August 5, 2003Assignee: Maxygen, Inc.Inventors: Willem P. C. Stemmer, Andreas Crameri
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Patent number: 6596539Abstract: The invention relates to a method and compositions for modifying a phenotype of a virus, such as viral tropism and host range, by iterative sequence recombination of variant viruses and selection of improved variants.Type: GrantFiled: October 30, 1998Date of Patent: July 22, 2003Assignee: Maxygen, Inc.Inventors: Willem P. C. Stemmer, Phillip Patten, Nay Wei Soong
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Patent number: 6586182Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.Type: GrantFiled: October 20, 2000Date of Patent: July 1, 2003Assignee: Maxygen, Inc.Inventors: Phillip A. Patten, Willem P. C. Stemmer
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Patent number: 6579678Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.Type: GrantFiled: October 20, 2000Date of Patent: June 17, 2003Assignee: Maxygen, Inc.Inventors: Phillip A. Patten, Willem P. C. Stemmer
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Patent number: 6576757Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.Type: GrantFiled: November 28, 2000Date of Patent: June 10, 2003Assignee: Maxygen, Inc.Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
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Patent number: 6576467Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: November 21, 2000Date of Patent: June 10, 2003Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
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Patent number: 6573098Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: January 29, 1999Date of Patent: June 3, 2003Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
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Patent number: 6569435Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.Type: GrantFiled: November 28, 2000Date of Patent: May 27, 2003Assignee: Maxygen, Inc.Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
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Patent number: 6555660Abstract: The invention relates to polypeptide conjugates comprising a polypeptide exhibiting G-CSF activity and having an amino acid sequence that differs from the amino acid sequence of human G-CSF in at least one specified introduced and/or removed amino acid residue comprising an attachment group for a non-polypeptide moiety, and having at least one non-polypeptide moiety attached to an attachment group of the polypeptide. The attachment group may e.g. be a lysine, cysteine, aspartic acid or glutamic acid residue or a glycosylation site, and the non-polypeptide moiety may e.g. be a polymer such as polyethylene glycol or an oligosaccharide. The conjugate, which has a reduced in vitro bioactivity compared to hG-CSF, has one or more improved properties such as increased biological half-life and increased stimulation of neutrophils.Type: GrantFiled: July 11, 2001Date of Patent: April 29, 2003Assignee: Maxygen Holdings Ltd.Inventors: Torben Lauesgaard Nissen, Kim Vilbour Andersen, Christian Karsten Hansen, Jan Moller Mikkelsen, Hans Thalsgaard Schambye
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Patent number: 6541011Abstract: This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.Type: GrantFiled: February 10, 1999Date of Patent: April 1, 2003Assignee: Maxygen, Inc.Inventors: Juha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer