Patents Represented by Attorney, Agent or Law Firm Norman J. Kruse
  • Methods and compositions for cellular and metabolic engineering
    Patent number: 6391640
    Abstract: The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.
    Type: Grant
    Filed: November 9, 1998
    Date of Patent: May 21, 2002
    Assignee: Maxygen, Inc.
    Inventors: Jeremy Minshull, Willem P. C. Stemmer
  • Evolution of whole cells and organisms by recursive sequence recombination
    Patent number: 6379964
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Grant
    Filed: July 15, 1999
    Date of Patent: April 30, 2002
    Assignee: Maxygen, Inc.
    Inventors: Stephen del Cardayre, Matthew Tobin, Willem P. C. Stemmer, Jeremy Minshull
  • Oligonucleotide mediated nucleic acid recombination
    Patent number: 6376246
    Abstract: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
    Type: Grant
    Filed: September 28, 1999
    Date of Patent: April 23, 2002
    Assignee: Maxygen, Inc.
    Inventors: Andreas Crameri, Willem P. C. Stemmer, Jeremy Minshull, Steven H. Bass, Mark Welch, Jon E. Ness, Claes Gustafsson, Phillip A. Patten
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6372497
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: June 8, 2000
    Date of Patent: April 16, 2002
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Oligonucleotide mediated nucleic acid recombination
    Patent number: 6368861
    Abstract: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
    Type: Grant
    Filed: January 18, 2000
    Date of Patent: April 9, 2002
    Assignee: Maxygen, Inc.
    Inventors: Andreas Crameri, Willem P. C. Stemmer, Jeremy Minshull, Steven H. Bass, Mark Welch, Jon E. Ness, Claes Gustafsson, Phillip A. Patten
  • Recombination of insertion modified nucleic acids
    Patent number: 6365377
    Abstract: Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.
    Type: Grant
    Filed: March 3, 2000
    Date of Patent: April 2, 2002
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Volker Heinrichs, Willem P. C. Stemmer
  • Recombination of insertion modified nucleic acids
    Patent number: 6358740
    Abstract: Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.
    Type: Grant
    Filed: November 27, 2000
    Date of Patent: March 19, 2002
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Volker Heinrichs, Willem P. C. Stemmer
  • Methods and compositions for polypeptides engineering
    Patent number: 6355484
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: June 24, 1999
    Date of Patent: March 12, 2002
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Evolution of whole cells and organisms by recursive sequence recombination
    Patent number: 6352859
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Grant
    Filed: July 26, 2000
    Date of Patent: March 5, 2002
    Assignee: Maxygen, Inc.
    Inventors: Stephen delCardayre, Matthew Tobin, William P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswatan Subramanian, Linda Castle, Claus M. Krebber, Steve Bass
  • Methods for recombining nucleic acids
    Patent number: 6344356
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: June 8, 2000
    Date of Patent: February 5, 2002
    Assignee: Maxygen, Inc.
    Inventor: Willem P.C. Stemmer
  • Method for producing polynucleotides with desired properties
    Patent number: 6337186
    Abstract: The invention provides methods for the production of polynucleotides with a desired property (e.g., conferring a desired phenotype and/or encoding polypeptide with a desired property) which is selectable or can be screened for. The method includes making insertions and/or deletions at random sites in DNA segments in a population. In some embodiments the random insertions and deletions are made recursively.
    Type: Grant
    Filed: June 15, 1999
    Date of Patent: January 8, 2002
    Assignee: Maxygen, Inc.
    Inventor: Claus M. Krebber
  • Methods and compositions for polypeptide engineering
    Patent number: 6335160
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: December 18, 1996
    Date of Patent: January 1, 2002
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Evolution of whole cells and organisms by recursive sequence recombination
    Patent number: 6335198
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Grant
    Filed: July 26, 2000
    Date of Patent: January 1, 2002
    Assignee: Maxygen, Inc.
    Inventors: Stephen delCardayre, Matthew Tobin
  • Evolution of whole cells and organisms by recursive sequence recombination
    Patent number: 6326204
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Grant
    Filed: July 15, 1998
    Date of Patent: December 4, 2001
    Assignee: Maxygen, Inc.
    Inventors: Stephen delCardayre, Matthew Tobin, William P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswatan Subramanian, Linda Castle, Steve Bass
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6323030
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: January 29, 1999
    Date of Patent: November 27, 2001
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Methods and compositions for polypeptide engineering
    Patent number: 6319713
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: June 25, 1999
    Date of Patent: November 20, 2001
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P. C. Stemmer
  • Oligonucleotide mediated nucleic acid recombination
    Patent number: 6319714
    Abstract: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: November 20, 2001
    Assignee: Maxygen, Inc.
    Inventors: Andreas Crameri, Willem P.C. Stemmer, Jeremy Minshull, Steven H. Bass, Mark Welch, Jon E. Ness, Claes Gustafsson, Phillip A. Patten
  • Methods and compositions for polypeptide engineering
    Patent number: 6303344
    Abstract: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.
    Type: Grant
    Filed: June 24, 1999
    Date of Patent: October 16, 2001
    Assignee: Maxygen, Inc.
    Inventors: Phillip A. Patten, Willem P.C. Stemmer
  • Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
    Patent number: 6297053
    Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
    Type: Grant
    Filed: February 10, 2000
    Date of Patent: October 2, 2001
    Assignee: Maxygen, Inc.
    Inventor: Willem P. C. Stemmer
  • Evolution of whole cells and organisms by recursive sequence recombination
    Patent number: 6287862
    Abstract: The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.
    Type: Grant
    Filed: July 26, 2000
    Date of Patent: September 11, 2001
    Assignee: Maxygen, Inc.
    Inventors: Stephen delCardayre, Matthew Tobin, Willem P. C. Stemmer, Jon E. Ness, Jeremy Minshull, Phillip Patten, Venkiteswaran Subramanian, Linda Castle, Claus M. Krebber, Steven H. Bass