Abstract: A population of T lymphocyte precursor cells is disclosed. In bone marrow, the earliest identifiable T lymphocyte precursor is CD34.sup.+, CD7.sup.+ and Leu 8.sup.+++. Methods of isolation and methods of therapeutic use of such cells also are disclosed.

Abstract: A chromatographic test strip comprising a solid support having at least a first portion and a second portion with said portions being in the same plane so as to permit capillary flow communication with each other. The sample is added to the first portion. The first portion also may comprise a tracer portion having a tracer movably supported therein. The tracer consists of a visible particulate marker. In the second portion, a binder is immobilized. The test strip is useful in a variety of immunoassays.

Abstract: A method for prognosticating the effectiveness of a chemotherapy using monoclonal antibodies and ligand molecules. The putative anti-cancer agent has binding specificity for a oncogenic receptor molecule on the membrane of a cancer cell, such as HER-2/neu. When the putative agent binds to the oncogenic receptor, the receptor translocates from the membrane to the cytoplasm or perinucleus of the cancer cell, accompanied by a transient increase in the total cellular content of the receptor, and results in terminal cell differentiation. The efficacy of the agent in vivo can be determined in vitro by treatment of biopsied cancer cells with the agent and subsequent examination of the cells for evidence of terminal cell differentiation. Such evidence includes morphological change, reduction in cell growth, or production of chemicals associated with the mature phenotype.

Abstract: A method for classifying and monitoring leukemias is disclosed. The method comprises mixing cells from a patient with a plurality of fluorescently labelled monoclonal antibodies, examining the cells by means of flow cytometry, scoring the percent of positive cells in each quadrant in a two-dimensional plot of log fluorescence and comparing the scores with a standard. Monitoring of the disease also may be achieved by comparing the scores before, during and after treatment.

Abstract: A method for the non-linear, non-logrithmic transformation of one or more parameters of data gathered by means of flow cytometry is disclosed wherein the method seeks to maximize the resolution between populations of interest for each parameter while maintaining the dynamic range of the data recorded.

Abstract: A method is disclosed for the treatment and prevention of graft versus host disease in man through the combined use of anti-CD8 monoclonal antibodies and a CD4.sup.+ cell inactivator.

Abstract: An antigen/antibody complex is described wherein the antibody functions as an adjuvant. The antibody comprises an anti-lymphocyte antibody. The antigen comprises a hapten, peptide, protein, carbohydrate, virus, bacterium, parasite or other whole microorganism. The complex of antigen coupled to antibody may be used in immunizing a higher animal against an antigen.

Abstract: The present invention comprises a method for detecting immunological changes in blood samples from patients infected with HIV. The method comprises the use of fluorescently labelled monoclonal antibodies against IL-2R (CD25). Other antibodies, including anti-CD3, anti-CD38 and anti-HLA DR antibodies also may be used. It has been found that a decrease in expression of CD25 in such samples indicates HIV infection.

Abstract: A method and kit for identification of the lineages and stages of hematopoietic cells in a sample wherein the cells are treated with labeled monoclonal antibodies selected to distinguish between each lineage and maturational stages within each lineage.

Abstract: A novel protein complex is disclosed which is co-associated with CD3.zeta. on CD16.sup.- NK cells. A purified form of the protein complex, a gene encoding the protein complex and method of producing the purified protein complex also are disclosed. Antibodies that bind to the protein complex and methods for their use further are disclosed.

Abstract: A method for discriminating between patients who are seropositive but asymptomatic for HIV infection and AIDS patients is disclosed wherein the fluorescence intensity from CD.sub.44.sup.+ T cells is measured. Intensity increases as AIDS progresses.

Abstract: A material and method are described to reduce the non-specific binding of a labelled material, wherein the labelled material comprises a fluorochrome and a specific binding pair member. The invention is particularlly suited to the lysis of peripheral blood wherein a labelled material is used to identify and detect cell populations within a sample of blood.

Abstract: A method for cleaning cellular contamination in a flow cytometer comprising the sequential use of a strong oxidizing solution, particle-free neutral pH fluid and a weak acid.

Abstract: Monoclonal antibodies which recognize activated T lymphocytes are secreted by hybridomas produced by conventinal fusion and selection methodology following immunization of mice with a human lymphocyte fraction containing T lymphocytes activated by a mitrogen or an antigen. The monoclonal antibodies are used in a method to monitor subsets of activated T lymphocytes present, for example, from a patient's blood.

Abstract: Novel xanthene dyes are disclosed which excite in the range of 450-650 nm and which will emit to the red of fluoroscein. These dyes may be coupled to tagging agents, such as monoclonal antibodies, and used to detect cells in a sample.

Abstract: A kit for determination of subsets of leukocytes utilizing flow cytometry analysis techniques.

Abstract: A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.

Abstract: L60 is a novel monoclonal antibody that will react with Leu 22. It is equally reactive in formalin, ZnSO.sub.4 or Bouin's fixed, paraffin-embedded tissues as well as in frozen tissues.

Abstract: A method for multi-parameter analysis of cells in a body fluid sample is described which makes use of a plurality of fluorescence measurements, comprising at least two nucleic acid dyes and at least one fluorescently labelled cell surface marker, and a plurality of light scattering measurements. A kit containing the nucleic acid dyes and cell surface marker also is described.

Abstract: A method is disclosed for removing residual tumor cells from a patient with a neoplastic disorder by treating in vitro cell preparation with lymphokine activated cytotoxic cells.