Abstract: A non-ionic lytic agent, preferably saponin for reducing the background carbon dioxide produced by blood cell metabolism is used in the testing of cultures for the presence of microorganisms. The hemolytic agent saponin is combined with a phospholipid, preferably L-.alpha.-Lecithin (phosphatidylcholine), to form mixed micelles which protect saponin from the effects of heat sterilization and high blood cholesterol levels, thus maintaining the lytic activity of saponin. The phospholipid/saponin mixed micelles are added to non-radiometric culture media vials such as Bactec.RTM. NR6A, NR7A and NR8A. The media vials are used in the Bactec.RTM. NR-660 and NR-730 instruments. However, the present invention may also be used in radiometric media such as Bactec.RTM. culture vials 6, 7 and 8 for reducing background carbon dioxide levels detected in C.sup.14 radiometric instruments such as the Bactec.RTM.

Abstract: A method and kit for determination of subsets of leukocytes utilizing flow cytometry analysis techniques.

Abstract: Reticulocytes, RNA or DNA are stained with a dye for detection in a flow cytometer. The dye has the formula: ##STR1## Wherein X=O, S, se or C(CH.sub.3).sub.2 ;R.sub.1 =alkyl having from 1-6 carbons;R.sub.2 =alkyl having from 1-6 carbons;R.sub.3 =fused benzene, alkyl (having 1-6 carbons), methoxy or is absent;R.sub.4 =alkyl having 1-6 carbons, methoxy or is absent; andn=zero or an integer from 1-6.

Abstract: L60 is a novel monoclonal antibody that will react with Leu 22. It is equally reactive in formalin, ZnSO.sub.4 or Bouin's fixed, paraffin-embedded tissues as well as in frozen tissues.

Abstract: A novel fluorescent dye, PUR-1, having the structural formula ##STR1## is disclosed that will preferentially stain nucleic acids.

Abstract: Novel xanthene dyes are disclosed which excite in the range of 450-650 nm and which will emit to the red of fluoroscein. These dyes may be coupled to tagging agents, such as monoclonal antibodies, and used to detect cells in a sample.

Abstract: A lysing agent and a method for utilizing the lysing agent in the identification and enumeration of cells of a select subclass of leucocytes is provided. The lysing agent includes formaldehyde, an alkali or alkaline earth salt of a weak acid and a polyhydric alcohol.

Abstract: A method is described for the detection of halodeoxyuridines taken up by cells during DNA synthesis by the use of monoclonal antibodies. The described method also allows the simultaneous detection of cell surface receptors.

Abstract: Reticulocytes, RNA or DNA are stained with a dye for detection in a flow cytometer. The dye has the formula: ##STR1## Wherein: X=O,S, Se, N -alkyl (having 1-6 carbons) or C (CH.sub.3).sub.n ; R.sub.1 =alkyl having from 1-6 carbons;R.sub.2 =alkyl having from 1-6 carbons;R.sub.3 =fused benzene, alkyl (having 1-6 carbons), methoxy or is absent;R.sub.4 =alkyl having 1-6 carbons, methoxy or is absent; andn=zero or an integer from 1-6.

Abstract: A method for calibrating an instrument for using that instrument to obtain at least one light-related signal from particles under analysis comprises directing an incident beam of light at calibration particles having one or more known characteristics related to the particles expected to be analyzed. Both a light signal and a noise signal from the calibration particles are detected. A measurement is made of the ratio of the detected light signal to the detected noise signal, and that measurement is reported. The measured ratio is then compared to a predetermined ratio which represents a threshold for minimum instrument performance. This method further includes adjusting, if the predetermined ratio has not been attained, the operation of the instrument, while the calibration particles are within the incident beam of light, until the measured ratio reaches the predetermined ratio whereby the instrument is calibrated for subsequently obtaining the light signal from particles to be analyzed.

Abstract: A method for detection of DNA sequences. In the method, chromatin (comprising protein and DNA) is contacted with a cross-linking agent for the protein of the chromatin to provide a substantially rigid chromatin particle. The DNA of the chromatin particle is then subjected to treatment to cause a separation of the individual DNA strands into single stranded DNA. Preselected sequences of the single stranded DNA are then contacted with a complementary polynucleotide probe specific for the DNA sequence of interest. The polynucleotide probe is marked with a fluorescent label thereby labelling a target sequence of DNA in the chromatin particles. The fluorescently tagged DNA sequences are then detected by subjecting the polynucleotide probe to a suitable light source and detecting the light emitted by the fluorescent label so as to identify the preselected DNA sequence.

Abstract: A pipe aligner for holding a metal pipe securely for welding which comprises a back, two arms that wrap around the pipe and means to secure the pipes to the arms and back.

Abstract: A method for identifying and enumerating cells of a subclass of blood cells in relation to cells of another subclass of blood cells is provided. In the method a first subclass of blood cells is selectively tagged by incubating an aliquot of a blood sample with a first tagging agent. A second subclass of blood cells is selectively tagged by incubating the aliquot with a second tagging agent. The aliquot is then passed, without lysing of any subclasses of blood cells which are not of interest, substantially a cell at a time through an area of optical stimulation for the tagging agents. Light emitted by the tagging agents is detected, the detection being limited by gating to a threshold value related to a predetermined intensity of light by one of the tagging agents. Cells of the subclass are differentiated based on occurrence of emitted light from the tagging agents.

Abstract: An inexpensive method and composition for the manufacture of large sized, high gradient index glass using a sol-gel diffusion process in which the ions that form the metal oxides are of unequal valence.

Abstract: Highly purified, biologically active Human Factor VIII:C having specific activities of about 4000-8000 units per milligram of protein is prepared. In the method of preparation, an AHF concentrate is solubilized or equilibrated in an aqueous medium and treated to change the effective Stokes' radius of the Factor VIII:C to an apparently low value and then subjected to a separation from the concentrate. Treatment of the highly purified Factor VIII:C with a mixture of glycosidases causes substantial removal of carbohydrate side chains without reduction of procoagulant activity and with retention of significant in vivo survival time.

Abstract: A submersible mixer with a housing, within which housing sits a motor and gear box or other drive means, attached to said housing is a high-efficiency impeller, said housing being supported by vanes which baffle liquid flow when being mixed, and which when said impeller axis is aligned vertically form the base, and which when said impeller axis is aligned horizontally form two points of a tripod.