Abstract: The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the target analyte, either directly or indirectly, to allow electronic detection of the ETM.
Type:
Grant
Filed:
June 23, 1999
Date of Patent:
July 24, 2001
Assignee:
Clinical Micro Sensors, Inc.
Inventors:
Gary Blackburn, Stephen E. Creager, Scott Fraser, Bruce D. Irvine, Thomas J. Meade, Stephen D. O'Connor, Robert H. Terbrueggen, Jost G. Vielmetter, Thomas W. Welch
Abstract: The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, &lgr;, of an electron transfer process.
Abstract: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.
Type:
Grant
Filed:
July 24, 1997
Date of Patent:
April 24, 2001
Assignee:
Clinical Micro Sensors, Inc.
Inventors:
Jon Faiz Kayyem, Stephen D. O'Connor, Michael Gozin, Changjun Yu, Thomas J. Meade
Abstract: The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Type:
Grant
Filed:
December 6, 1999
Date of Patent:
March 13, 2001
Assignee:
California Institute of Technology
Inventors:
Thomas J. Meade, Jon Faiz Kayyem, Scott E. Fraser
Abstract: An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
Type:
Grant
Filed:
April 21, 1999
Date of Patent:
March 6, 2001
Assignee:
Harvard University
Inventors:
Cynthia C. Bamdad, George B. Sigal, Jack L. Strominger, George M. Whitesides
Abstract: The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Type:
Grant
Filed:
December 10, 1999
Date of Patent:
January 30, 2001
Assignee:
California Insitute of Technology
Inventors:
Thomas J. Meade, Jon Faiz Kayyem, Scott E. Fraser
Abstract: The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Type:
Grant
Filed:
May 7, 1999
Date of Patent:
January 23, 2001
Assignee:
California Institute of Technology
Inventors:
Thomas J. Meade, Jon Faiz Kayyem, Scott E. Fraser