Patents Represented by Attorney, Agent or Law Firm Robin M. Silva
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Patent number: 6514729Abstract: The invention relates to novel interferon-beta activity (IbA) proteins and nucleic acids. The invention further relates to the use of the IbA proteins in the treatment of IFN-&bgr; related disorders.Type: GrantFiled: May 11, 2000Date of Patent: February 4, 2003Assignee: Xencor, Inc.Inventor: Jörg Bentzien
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Patent number: 6495323Abstract: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.Type: GrantFiled: September 12, 2000Date of Patent: December 17, 2002Assignee: Clinical Micro Sensors, Inc.Inventors: Jon Faiz Kayyem, Stephen D. O'Connor
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Patent number: 6482593Abstract: The present invention provides biosensors, apparatus and methods for selectively detecting at least one complementary oligonucleotide target specie in a fluid sample containing a mixture of different oligonucleotide fragments. One preferred embodiment of the biosensor is as a unitary fiber optic array having species of single stranded nucleic acid disposed as individual deposits in aligned organization upon multiple strand end faces at differing spatial positions on the distal array end surface.Type: GrantFiled: November 5, 1998Date of Patent: November 19, 2002Assignee: Trustees of Tufts CollegeInventors: David R. Walt, Brian G. Healey
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Patent number: 6479240Abstract: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.Type: GrantFiled: May 22, 2000Date of Patent: November 12, 2002Assignee: Clinical Micro Sensors, Inc.Inventors: Jon F. Kayyem, Stephen D. O'Connor, Michael Gozin, Changjun Yu
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Patent number: 6472148Abstract: An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.Type: GrantFiled: January 21, 1997Date of Patent: October 29, 2002Assignee: President and Fellows of Harvard CollegeInventors: Cynthia C. Bamdad, George B. Sigal, Jack L. Strominger, George M. Whitesides
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Patent number: 6461813Abstract: The invention related to novel methods of detecting alterations in cell cycle regulation in a cell or a cell population and screening for agents capable of modulating cell cycle regulation through the use of multiparameter assays and a fluorescence-activated cell sorter (FACS) machine.Type: GrantFiled: September 21, 1998Date of Patent: October 8, 2002Assignee: Rigel Pharmaceuticals, Inc.Inventor: James Lorens
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Patent number: 6455247Abstract: Methods and compositons for screening for transdominant effector peptides and RNA molecules selected inside living cells from randomized pools are provided.Type: GrantFiled: January 23, 1997Date of Patent: September 24, 2002Assignees: Board of Trustees of the Leland Stanford Junior University, Rigel Pharmaceuticals, Inc.Inventors: Garry P. Nolan, S. Michael Rothenberg
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Patent number: 6455263Abstract: The present invention relates to methods of screening libraries of small molecules such as combinatorial chemical libraries of organic molecules, including peptides and other chemical libraries, for binding to target molecules, using fluoroscence-activated cell sorting (FACS) machines.Type: GrantFiled: March 24, 1998Date of Patent: September 24, 2002Assignee: Rigel Pharmaceuticals, Inc.Inventor: Donald Payan
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Patent number: 6447765Abstract: The field of the invention is generally related to pharmaceutical agents useful in treating graft-versus-host disease (GVHD) in patients that have received allogenic bone marrow transplants.Type: GrantFiled: March 3, 1999Date of Patent: September 10, 2002Assignee: University of Southern CaliforniaInventor: David A. Horwitz
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Patent number: 6444423Abstract: The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.Type: GrantFiled: November 13, 1998Date of Patent: September 3, 2002Assignee: Molecular Dynamics, Inc.Inventors: Thomas J. Meade, Thomas W. Welch
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Patent number: 6432723Abstract: A method for detecting the presence of a target analyte comprising binding a target analyte to a binding ligand comprising at least a first electron donor moiety and a second electron acceptor moiety; and detecting the electron transfer between the donor and acceptor, wherein there is a change in the amount of electron transfer between the donor and acceptor as a result of altering the structured state of the donor and acceptor caused by a conformational change in the binding ligand upon binding of the target ligand.Type: GrantFiled: January 21, 2000Date of Patent: August 13, 2002Assignee: Clinical Micro Sensors, Inc.Inventors: Kevin W. Plaxco, Jon Faiz Kayyem
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Patent number: 6428980Abstract: The present invention is directed to novel polypeptides, nucleic acids and related molecules which have an effect on or are related to the cell cycle. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are methods for identifying novel compositions which mediate cell cycle bioactivity, and the use of such compositions in diagnosis and treatment of disease.Type: GrantFiled: November 16, 1999Date of Patent: August 6, 2002Assignee: Rigel Pharmaceuticals, Inc.Inventors: Ying Luo, PeiWen Yu, Mary Shen, Betty Huang
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Patent number: 6429027Abstract: The invention relates to compositions and methods for decoding microsphere array sensors.Type: GrantFiled: February 24, 1999Date of Patent: August 6, 2002Assignee: Illumina, Inc.Inventors: Mark S. Chee, Steven R. Auger, John R. Stuelpnagel
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Patent number: 6406845Abstract: The present invention provides biosensors, apparatus and methods for selectively detecting at least one complementary oligonucleotide target specie in a fluid sample containing a mixture of different oligonucleotide fragments. One preferred embodiment of the biosensor is as a unitary fiber optic array having an in-situ hybridization zone comprising not less than one specie of single stranded oligonucleotide disposed as individual deposits in aligned organization upon multiple strand end faces at differing spatial positions on the distal array end surface. In this manner, a collective of deployed, single specie, multiple fixed probes are presented for selective in-situ hybridization on-demand with at least one mobile complementary target specie ultimately bearing a joined identifying label. The biosensor provides for optical detection of in-situ hybridization on the distal end surface via the presence of the concomitantly disposed joined identifying label at the differing spatial positions.Type: GrantFiled: May 5, 1997Date of Patent: June 18, 2002Assignee: Trustees of Tuft CollegeInventors: David R. Walt, Brian G. Healey
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Patent number: 6403312Abstract: The invention relates to the use of protein design automaton (PDA) to generate computationally prescreened secondary libraries of proteins, and to methods and compositions utilizing the libraries.Type: GrantFiled: October 15, 1999Date of Patent: June 11, 2002Assignee: XencorInventors: Bassil I. Dahiyat, Robert J. Hayes, Jörg Bentzien, Klaus M. Fiebig
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Patent number: 6391582Abstract: The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.Type: GrantFiled: December 9, 1998Date of Patent: May 21, 2002Assignee: Rigel Pharmaceuticlas, Inc.Inventors: Ying Luo, Pei Wen Yu, James Lorens
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Patent number: 6387658Abstract: The present invention is directed to novel polypeptides, nucleic acids and related molecules which have an effect on or are related to the cell cycle. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are methods for identifying novel compositions which mediate cell cycle bioactivity, and the use of such compositions in diagnosis and treatment of disease.Type: GrantFiled: September 23, 1999Date of Patent: May 14, 2002Assignee: Rigel Pharmaceuticals, Inc.Inventors: Ying Luo, Betty Huang
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Patent number: 6377721Abstract: A biosensor, sensor array, sensing method and sensing apparatus are provided in which individual cells or randomly mixed populations of cells, having unique response characteristics to chemical and biological materials, are deployed in a plurality of discrete sites on a substrate. In a preferred embodiment, the discrete sites comprise microwells formed at the distal end of individual fibers within a fiber optic array. The biosensor array utilizes an optically interrogatable encoding scheme for determining the identity and location of each cell type in the array and provides for simultaneous measurements of large numbers of individual cell responses to target analytes. The sensing method utilizes the unique ability of cell populations to respond to biologically significant compounds in a characteristic and detectable manner.Type: GrantFiled: March 2, 1999Date of Patent: April 23, 2002Assignee: Trustees of Tufts CollegeInventors: David R. Walt, Laura C. Taylor
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Patent number: 6365344Abstract: Biochemical libraries are screened for transdominant intracellularly bioactive agents by expressing a molecular library of randomized nucleic acids as a plurality of corresponding expression products in a plurality of cells, each of the nucleic acids comprising a different nucleotide sequence, detecting a cell of the plurality of cells exhibiting a changed physiology in response to the presence in the cell of a transdominant expression product of the corresponding expressio products; and isolating the cell and/or transdominant expression product.Type: GrantFiled: January 23, 1996Date of Patent: April 2, 2002Assignees: The Board of Trustees of the Leland Stanford Junior University, Rigel Pharmaceuticals, Inc.Inventors: Garry P. Nolan, S. Michael Rothenberg
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Patent number: 6361958Abstract: The invention relates to a microfluidic device with microchannels that have separated regions which have a member of a specific binding pair member such as DNA or RNA bound to porous polymer, beads or structures fabricated into the microchannel. The microchannels of the invention are fabricated from plastic and are operatively associated with a fluid propelling component and detector.Type: GrantFiled: November 12, 1999Date of Patent: March 26, 2002Assignee: Motorola, Inc.Inventors: Chan-Long Shieh, Barbara Foley, Huinan Yu, Vi-En Choong