Patents Assigned to Agilent Technologies, Inc.
  • METHODS AND KITS TO IMPROVE THE FLUORESCENT SIGNAL OF DMB-LABELED SIALIC ACIDS
    Publication number: 20220242896
    Abstract: The present invention provides methods and kits to improve the signal from sialic acids labeled with the dye 1, 2-diamino-4, 5-methylenedioxybenzene (“DMB”). The methods include labeling the sialic acids with DMB in an aqueous solution comprising the amino acid glycine, with pH adjusted to 1.5-3.2 by an acid, such as phosphoric acid or hydrochloric acid, and a reductant.
    Type: Application
    Filed: June 6, 2020
    Publication date: August 4, 2022
    Applicant: Agilent Technologies, Inc.
    Inventor: Francis T. Haxo
  • Synthesis of pools of probes by primer extension
    Patent number: 11390907
    Abstract: Provided herein is a method for making a pool of probes by primer extension. In certain embodiments, the method comprises hybridizing a first population of oligonucleotides comprising a top strand sequence having the following formula V1-B-3? with a second population of oligonucleotides comprising a bottom strand sequence having the following formula V2?-B?-3? to provide a population of duplexes. After hybridizing, the 3? ends of the oligonucleotides in the duplexes are extended to produce a population of double stranded products comprising a top strand sequence having the following formula V1-B-V2, where V2 is complementary to V2?.
    Type: Grant
    Filed: March 5, 2013
    Date of Patent: July 19, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Michael Ruvolo, Emily Marine Leproust
  • SPATIAL NUCLEIC ACID DETECTION USING OLIGONUCLEOTIDE MICROARRAYS
    Publication number: 20220220544
    Abstract: The present disclosure is generally directed to detecting nucleic acids. In particular, disclosed herein are methods and compositions for determining the sequence (or identity) and location of RNA and other molecules in situ. The present invention is generally related to a method for detecting nucleic acids, the method including providing a tissue sample; providing an array comprising a plurality of oligonucleotide probes attached to a surface of the array, in which each oligonucleotide probe, of the plurality of oligonucleotide probes, includes a location barcode sequence, a primer binding sequence, and a priming sequence; releasing the plurality of oligonucleotide probes from the array surface; contacting the tissue sample with the released oligonucleotide probes; and allowing the released oligonucleotide probes to diffuse into the tissue sample.
    Type: Application
    Filed: January 7, 2022
    Publication date: July 14, 2022
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: Robert A. ACH, Nicholas M. SAMPAS, Brian Jon PETER
  • METHODS TO IMPROVE DETECTION OF GLYCOSYLAMINES
    Publication number: 20220214334
    Abstract: The present invention provides methods to improve the sensitivity of detecting glycosylamines released from glycoconjugates, such as glycoproteins or glycopeptides, by enzymatic digestion when labeling them with amine-reactive dyes.
    Type: Application
    Filed: April 30, 2020
    Publication date: July 7, 2022
    Applicant: Agilent Technologies, Inc.
    Inventor: Andres Guerrero Navarro
  • Devices and kits to improve reduction or labeling of carbohydrates
    Patent number: 11371998
    Abstract: The present invention provides methods, devices, and kits to improve procedures for reducing carbohydrates, such as glycans released from glycoconjugates, or for labeling carbohydrates by reductive amination.
    Type: Grant
    Filed: September 17, 2019
    Date of Patent: June 28, 2022
    Assignee: Agilent Technologies, Inc.
    Inventor: Andres Guerrero Navarro
  • Branching off fluidic sample with low influence on source flow path
    Patent number: 11371968
    Abstract: A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured for adjusting a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.
    Type: Grant
    Filed: July 1, 2020
    Date of Patent: June 28, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Thomas Ortmann, Daniel Thielsch, Dominik Ruf
  • CHEMICALLY MODIFIED GUIDE RNAS FOR CRISPR/CAS-MEDIATED GENE REGULATION
    Publication number: 20220195425
    Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
    Type: Application
    Filed: March 14, 2022
    Publication date: June 23, 2022
    Applicants: The Board of Trustees of the Leland Stanford Junior University, Agilent Technologies, Inc.
    Inventors: Matthew H. Porteus, Ayal Hendel, Joe Clark, Rasmus O. Bak, Daniel E. Ryan, Douglas J. Dellinger, Robert Kaiser, Joel Myerson
  • COMPOSITIONS COMPRISING CHEMICALLY MODIFIED GUIDE RNAS FOR CRISPR/CAS-MEDIATED EDITING OF HBB
    Publication number: 20220195427
    Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
    Type: Application
    Filed: March 14, 2022
    Publication date: June 23, 2022
    Applicants: The Board of Trustees of the Leland Stanford Junior University, Agilent Technologies, Inc.
    Inventors: Matthew H. Porteus, Ayal Hendel, Joe Clark, Rasmus O. Bak, Daniel E. Ryan, Douglas J. Dellinger, Robert Kaiser, Joel Myerson
  • FILTER ASSEMBLIES, DEPTH INDICATORS, TORQUE-LIMITING FITTINGS, TORQUE-INDICATING FITTINGS, AND SYSTEMS INCORPORATING THE SAME
    Publication number: 20220196612
    Abstract: In some examples, a filter assembly may include a filter including a first gasket having a first channel adjacent to a first side of the filter and a second gasket having a second channel adjacent to a second side of the filter. The first gasket and the second gasket may include a beveled surface adjacent to the filter. The first channel and the second channel may include a diameter of from about 0.01 mm to about 0.5 mm. A finger tightening system may securely hold the filter without any leaks.
    Type: Application
    Filed: March 9, 2022
    Publication date: June 23, 2022
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: Alan D. LOUX, Lucas SERGE, Manuel VAN-VENROOY, Thomas HARRISON, Matthias KAMUF
  • CHEMICALLY MODIFIED GUIDE RNAS FOR CRISPR/CAS-MEDIATED GENE CORRECTION
    Publication number: 20220195426
    Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
    Type: Application
    Filed: March 14, 2022
    Publication date: June 23, 2022
    Applicants: The Board of Trustees of the Leland Stanford Junior Univerisity, Agilent Technologies, Inc.
    Inventors: Matthew H. Porteus, Ayal Hendel, Joe Clark, Rasmus O. Bak, Daniel E. Ryan, Douglas J. Dellinger, Robert Kaiser, Joel Myerson
  • FILTER ASSEMBLIES, DEPTH INDICATORS, TORQUE-LIMITING FITTINGS, TORQUE-INDICATING FITTINGS, AND SYSTEMS INCORPORATING THE SAME
    Publication number: 20220196613
    Abstract: In some examples, a filter assembly may include a filter including a first gasket having a first channel adjacent to a first side of the filter and a second gasket having a second channel adjacent to a second side of the filter. The first gasket and the second gasket may include a beveled surface adjacent to the filter. The first channel and the second channel may include a diameter of from about 0.01 mm to about 0.5 mm. A finger tightening system may securely hold the filter without any leaks.
    Type: Application
    Filed: March 9, 2022
    Publication date: June 23, 2022
    Applicant: Agilent Technologies, Inc.
    Inventors: Alan D. Loux, Lucas Serge, Manuel Van-Venrooy, Thomas Harrison, Matthias Kamuf
  • Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
    Patent number: 11360072
    Abstract: A system for monitoring cell-substrate impedance of excitable cells at millisecond time resolution and methods of assessing cell beating by monitoring cell-substrate impedance of beating cells at millisecond time resolution.
    Type: Grant
    Filed: January 3, 2020
    Date of Patent: June 14, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Xiaobo Wang, Yama A. Abassi, Biao Xi, Wen Fu Zhang, Xiao Xu
  • Ion pump shield
    Patent number: 11355327
    Abstract: An ion pump with a housing enclosing an interior, a gas inlet having a through-hole extending into the interior of the ion pump, at least one cathode, at least one anode positioned in proximity to the at least one cathode, a magnet disposed on an opposite side of the at least one cathode from the anode, and a blocking shield disposed between the gas inlet and the at least one cathode. The blocking shield is electrically connected to the at least one anode. An associated method installs the blocking shield by inserting components of the blocking shield assembly through the gas inlet, and assembling (inside the interior of the ion pump) the inserted components to form the blocking shield.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: June 7, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Cristian Maccarrone, Chiara Paolini, Enrica Carbonero, Paolo Manassero
  • System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
    Patent number: 11346797
    Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: May 31, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Xiaobo Wang, Wei Ouyang, Nan Li, Tianxing Wang, Xiaoyu Zhang, Xiao Xu, Yama A. Abassi
  • Branching off fluidic sample with low influence on source flow path
    Patent number: 11346822
    Abstract: A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured for adjusting a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.
    Type: Grant
    Filed: May 10, 2019
    Date of Patent: May 31, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Thomas Ortmann, Daniel Thielsch
  • Multi frequency LC resonator topologies applicable to mass spectrometer radio-frequency drive systems
    Patent number: 11342169
    Abstract: In one embodiment, a power source for providing high-voltage radio-frequency (RF) energy to an instrument such as a mass spectrometer includes an RF power amplifier having an output, an oscillating RF signal generator configured to provide first and second RF signals respectively oscillating at first and second frequencies to the RF power amplifier, and a step-up circuit for magnifying the RF power amplifier output. The step-up circuit includes an LC resonator network tuned to the first and second frequencies, and an output for providing the magnified voltage to a rod assembly of the mass spectrometer.
    Type: Grant
    Filed: April 8, 2020
    Date of Patent: May 24, 2022
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventor: Michael Schoessow
  • UV-absorbance multichannel capillary electrophoresis system
    Patent number: 11340191
    Abstract: A capillary electrophoresis system includes a capillary reservoir. The capillary reservoir includes a capillary tip flow chamber configured to receive respective capillary tips and to conduct fluid past the capillary tips, and an electrode flow chamber in which an electrode is disposed and configured to conduct fluid past the electrode, the electrode flow chamber being separate from and in fluid communication with the capillary tip flow chamber. An ultraviolet (UV) light absorbance-based multiplexed capillary electrophoresis system includes a first enclosure and a second enclosure. The first enclosure covers a UV light source, and includes a slit. The second enclosure covers the first enclosure, a collimating lens, and a capillary window.
    Type: Grant
    Filed: August 15, 2017
    Date of Patent: May 24, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Michael Stebniski, Bruce R. Boeke, Martin Chris Foster, Scott Stueckradt, Thomas J. Kurt
  • Orthoester compositions for affinity purification of oligonucleotides
    Patent number: 11299483
    Abstract: Compounds and methods for purifying oligonucleotides such as RNA and DNA. A target oligonucleotide is reacted with an orthoester linker comprising an affinity tag to form an orthoester oligonucleotide-orthoester linker conjugate which is subjected to a purification technique to separate the target oligonucleotide from impurities such as truncated oligonucleotides. The orthoester linker can be then removed under mild conditions to generate the target oligonucleotide in high purity.
    Type: Grant
    Filed: August 17, 2018
    Date of Patent: April 12, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Joel Myerson, Brian Smart
  • Mid-infrared scanning system
    Patent number: 11300773
    Abstract: An apparatus and method for generating a mid-infrared region image of a specimen are disclosed. The apparatus includes a mid-infrared region laser that generates a first light beam, and a stage adapted to carry a specimen to be scanned. An optical assembly focuses the first light beam to a point on the specimen. A first light detector measures a first intensity of light leaving the point on the specimen. A stage actuator assembly causes the specimen to move relative to the point in two dimensions. A controller forms a mid-infrared region image from the first intensity. The image can be based on reflected or transmitted light. The maximum size of the imaged area is determined by a scanning assembly that moves in a first direction relative to the stage, the stage moving in a direction orthogonal to the first direction.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: April 12, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Charles Hoke, Varun Raghunathan
  • Gas Leak Detector Cartridge
    Publication number: 20220099518
    Abstract: A gas leak detector cartridge includes a cartridge housing that is removably installed in a handheld device. The cartridge housing includes a sensor that detects a presence of a contaminant in a sample gas and is connected to a printed circuit board. The cartridge housing also includes a pump positioned in a manifold and introduces a sample gas to the sensor. The sensor is at least partially inserted into the manifold that includes the pump.
    Type: Application
    Filed: September 28, 2020
    Publication date: March 31, 2022
    Applicant: Agilent Technologies, Inc.
    Inventors: Chin-Seong Khor, Jon-Wen Tan, Heng-Dar Ling