Abstract: The present disclosure in some embodiments provides a flow cell sorting system, a focus detection method, as well as a fluidic chip. In one aspect, the fluidic chip comprises a cover sheet and one or more substrate layers, and one or more channels in each of the fluidic chips. In one aspect, the cells or other analytes in a sample are focused onto the same plane as they flow through the fluidic channel. In another aspect, the cells or other analytes on the same plane are irradiated with the same light intensity, for example, by a flat-top laser beam. This combination eliminates or reduces variation caused by cells at different positions receiving different intensities of the excitation light, and therefore improves accuracy of the flow cytometry analysis. In another aspect, a sorting apparatus is designed at the junction of the detection area and the outlet channel to separate the target cells.

Abstract: In some aspects, the present disclosure provides a magnetic rack comprising a lateral movement structure and/or a longitudinal movement structure. In particular embodiments, the lateral movement structure and/or longitudinal movement structure move one or more magnets to or away from a reaction tube, in order to control the magnetic forces exerted on microbeads in the reaction tube. The microbeads are attracted onto the reaction tube wall, thereby facilitating separation of the microbeads from a solution in the tube. In particular embodiments, the magnetic rack is used to extract or purify nucleic acid from a sample.

Abstract: A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. This technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.

Abstract: The present disclosure relates to a method for camera identification and detection on color features. In some aspects, the method comprises: 1) starting the camera, the image processing unit and the display unit at the beginning of testing; 2) using the camera to capture the color characteristic value; and 3) moving the untested object to the detection area of the camera for it to be detected, wherein the image processing unit extracts the mean color characteristic value from the color pixels of the detection area.

Abstract: In some aspects, the present disclosure relates to a method for identifying the type of the unit by camera.

Abstract: The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.

Abstract: The invention provides a method for identification of alleles. In this method, genomic DNA is used as target. Multiple allele-specific PCR amplification are carried out with a group of primers comprising one or more allele-specific primers for a target gene, a universal primer, and a common primer; and a DNA polymerase without 5? to 3? exonuclease activity. The PCR products are hybridized with tag probes immobilized on a DNA chip. Results are determined based on the signal intensity and the position of the probe immobilized on the array. Each allele-specific primer comprises a unique tag sequence at the 5? end. Each tag probe immobilized on the DNA chip comprises a sequence identical to its corresponding tag sequence; and each tag probe hybridizes only with the complementary sequence in the PCR amplification product.

Abstract: An interface device for bio-chip at least comprises an interface unit, said interface unit consisting of instrument interface layer, fluid channel layer and sample interface layer. The instrument interface layer has at least one instrument interface. The fluid channel layer has one hollow-out fluid channel. The sample interface layer has at least one sample interface, and both ends of said fluid channel connect to the sample interface and the instrument interface respectively. The interface device separates sample solutions from the instrument interface by gas or liquid in the fluid channel, thus to avoid direct contacts between sample solutions and instrument interface which will cause pollution, and omitting cleaning processes after using instruments. With simple structure, easy operation and low cost, it applies to chemistry field, biology field and medical analysis field.

Abstract: Provided is a microfluidic chip, which comprises a substrate and a cover sheet, wherein a microreactor array is arranged on the substrate and comprises at least one main channel (401) and at least two micro cells (402) connected to the main channel (401). The microfluidic chip also comprises at least one local temperature control device, which is used for heating the main channel (401) or cooling the micro cells (402). The use of the microfluidic chip ensures uniformity and independency of the micro cells (402). Also provided is an application of the microfluidic chip in biological detection or medical inspection.

Abstract: The invention provides methods and compositions for the diagnosis and prognosis of cervical intraepithelial neoplasia and cervical cancer. The methods comprise the step of determining the expression levels or genetic status of specific miRNAs.

Abstract: The present invention relates to a micro-volume liquid ejection system, including an air pressure module, a micro-ejection unit which is connected with the air pressure module by a conduit, and a control circuit which is connected with the air pressure module and the micro-ejection unit respectively. The air is used as the pressure medium, resulting in improved cleaning process and reduced sample waste. The present invention includes an electric control circuit to pick up sample, eject sample and clean the conduits automatically, and enables handling of a multiplicity of samples. The present invention can be used for transferring or dispensing micro volume liquid including biological liquid on a nL and ?L scale.

Abstract: Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.

Abstract: Systems, apparatus and methods for designing and operating a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. In one implementation, a method of designing a cell-electrode impedance sensor includes determining a cell free cell-electrode impedance and a cell covered cell-electrode impedance based on a design model for the cell-electrode impedance sensor, wherein the design model is based on one or more factors, the factors including properties and elements of a cell-electrode impedance measurement system, using the cell free cell-electrode impedance and the cell covered cell-electrode impedance to obtain a sensor sensitivity of the cell-electrode impedance measurement system, and choosing one or more design parameters of the cell-electrode impedance sensor in the cell-electrode impedance measurement system to maximize the sensor sensitivity.

Abstract: A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.

Abstract: The present invention provides a microfluidic device and its use for cell motility classification. The microfluidic device comprises a fluid inlet, a sample inlet and a channel connecting the fluid inlet and the sample inlet, wherein the channel comprises at least two sections of different sizes, and the channel allows fluid flow from the fluid inlet to the sample inlet.

Abstract: Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.

Abstract: The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5?-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5?-terminal of one PCR primer of each pair in extension.

Abstract: A testing method of nucleic acid binding protein based on biochip, comprises the following steps: 1. puts a plurality of groups solution including nucleic acid captured probes into biological sample including a plurality of nucleic acid binding protein to be test, and thus forming nucleic acid captured probe-nucleic acid binding protein complexes; such nucleic acid captured probe includes at least a segment of binding sequence which can bind with aimed nucleic acid binding protein; 2. separates such nucleic acid captured probe-nucleic acid binding protein complexes, then recoveries nucleic acid captured probes; 3. hybridizes the nucleic acid captured probes according to step 2 with a plurality of single strand blotting probes on biochip substrate; the sequence of such blotting probe compensates with such nucleic acid captured probe or one of its strand; 4. detects the result of hybridization.

Abstract: Methods and apparatus for designing and measuring a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. The method of designing a cell-electrode impedance sensor comprises: determining a cell free cell-electrode impedance and a cell-covered cell-electrode impedance; obtaining a sensor sensitivity of the cell-electrode impedance measurement system; and choosing one or more design parameters of the cell-electrode impedance sensor to maximize the sensor sensitivity. When the frequency of AC signal between electrodes ranges from 10 kHz to 40 kHz, the sensitivity of the sensor is maximized.

Abstract: The present invention relates to calibration slides for fluorescence detection instruments and processing method of making them. The aim of this invention is to disclose calibration slides with high photostability and long lifetime for fluorescence detection instruments. The calibration slides are fabricated by patterning calibration spot arrays of modified inorganic phosphors on the glass slide. The process for producing a calibration slide comprises the following procedure: 1) Dispersing the inorganic phosphors of rare-earth doped complex in water; 2) Patterning the array of the above suspension on the glass slide. The calibration slides in the present invention employ a very stable fluorescing material that is insensitive to photobleaching, has long lifetime and stability under mild storage condition. The calibration slides in the present invention can be used to calibrate and test for some fluorescence instruments, i.e.