Patents Assigned to Corbion Biotech, Inc.
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Publication number: 20200008445Abstract: Methods of making high-protein food products and low-pH food products produced by such methods are disclosed.Type: ApplicationFiled: June 20, 2019Publication date: January 9, 2020Applicant: Corbion Biotech, Inc.Inventors: Beata Klamczynska, Ana Teresita Echaniz, Ruolin Zhu
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Patent number: 10519204Abstract: A method for preparing a protein isolate of the biomass of microalgae of the genus Chlorella, includes the following steps: supplying a microalgal biomass produced by fermentation, washing the biomass so as to eliminate the soluble interstitial compounds and concentrating the biomass, mechanically grinding the washed and concentrated biomass in a horizontal ball grinder-type system in order to produce an emulsion, destructuring the emulsion thus produced, triple-phase separation so as to separate the soluble fraction from the fractions containing the lipids and the cell debris, recovery of the soluble fraction thus produced in order to produce the soluble protein isolate, then evaporation, pasteurization and atomization of the protein isolate.Type: GrantFiled: July 16, 2015Date of Patent: December 31, 2019Assignee: CORBION BIOTECH, INC.Inventor: Samuel Patinier
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Patent number: 10501722Abstract: The present invention relates to a method for optimising the downstream processing of a protein-rich microalgae biomass of the Chlorella genus previously prepared by fermentation in heterotrophic conditions and in the absence of light, comprising: 1) providing biomass comprising more than 50% protein by dry weight of biomass; next, at low temperature, carrying out the following steps: 2) harvesting the biomass at the end of fermentation, 3) washing and concentrating the biomass, 4) optionally, lysing the biomass, next, without low temperature stress, 5) optionally, concentrating the biomass slurry, 6) applying heat treatment, 7) drying the biomass obtained in this way in order to obtain the product, a step of adjusting the pH to 7 being applied before or after the heat treatment step 6).Type: GrantFiled: April 4, 2018Date of Patent: December 10, 2019Assignee: CORBION BIOTECH, INC.Inventors: Amandine Druon, Samuel Patinier, Béatrice Toursel
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Patent number: 10465159Abstract: Disclosed is a method of mechanically crushing microalgae cells of the genus Chlorella at an industrial scale, the mechanical crushing being carried out in a horizontal ball mill system. In the method, the balls have an apparent density of between 2 and 3.5 kg/l, the filling rate of the crushing chamber is greater than or equal to 80%, and preferably greater than or equal to 85%, and the mechanical crushing is carried out continuously by a series of successive passes.Type: GrantFiled: July 3, 2014Date of Patent: November 5, 2019Assignee: CORBION BIOTECH, INC.Inventors: Beatrice Toursel, Francois Delannoy, Samuel Patinier
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Patent number: 10376895Abstract: An agitator shaft (1) for a grinding mill, including: —an axle (2) intended to be driven in rotation, —a succession of flat agitator elements (3) and tubular spacers (4) mounted in alternation on the axle and in a stack along the axle (2), —end stops (5, 6), secured to the axle, that keep the stack of agitator elements and spacers under compression, characterized in that the tubular spacers (4) have orifices allowing the free circulation of material and fluids from the outside of the agitator shaft to the inside, and vice versa.Type: GrantFiled: June 23, 2015Date of Patent: August 13, 2019Assignee: CORBION BIOTECH, INC.Inventors: David Segard, Samuel Patinier
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Patent number: 10351814Abstract: The invention relates to a method for fermentative production, on an industrial scale, of lipid-rich biomass of microalgae of the Chlorella genus having acceptable sensory properties, characterized in that the dissolved oxygen availability in the fermenter is controlled by tracking the respiratory quotient of said microalgae.Type: GrantFiled: August 22, 2014Date of Patent: July 16, 2019Assignee: CORBION BIOTECH, INC.Inventors: Sylvain Delaroche, Marie Le Ruyet, Laurent Segueilha, Heike Jerosch, Amandine Druon
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Patent number: 10344305Abstract: Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.Type: GrantFiled: November 9, 2018Date of Patent: July 9, 2019Assignee: CORBION BIOTECH, INC.Inventors: Scott Franklin, Walter Rakitsky, George Rudenko, Xinhua Zhao, Felipe Arana Rodriguez, Wenhua Lu, Janice Wee
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Patent number: 10316299Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.Type: GrantFiled: April 10, 2018Date of Patent: June 11, 2019Assignee: CORBION BIOTECH, INC.Inventors: David Davis, George N. Rudenko, Aravind Somanchi, Jason Casolari, Scott Franklin, Aren Ewing
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Patent number: 10299500Abstract: Granules of protein-rich microalgal biomass flour having: a particle size distribution, measured on an LS laser granulometer of the COULTER® brand, with a Dmode of 60 to 300 ?m and a D4.3 of 70 to 420 ?m; an aerated density, measured on a HOSOKAWA Powder Characteristics Tester, of 0.60 to 0.70 g/mL, and a compressibility, measured on a HOSOKAWA Powder Characteristics Tester, of 15 to 25%.Type: GrantFiled: November 27, 2014Date of Patent: May 28, 2019Assignee: CORBION BIOTECH, INC.Inventors: Damien Passe, Francois Delannoy
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Patent number: 10287613Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.Type: GrantFiled: January 19, 2018Date of Patent: May 14, 2019Assignee: CORBION BIOTECH, INC.Inventors: Scott Franklin, Aravind Somanchi, George N. Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
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Patent number: 10264809Abstract: The present invention relates to microalgal food products with acceptable sensory characteristics and methods of producing the food products. The flour can be produced by cultivating microalgal cells of a strain of Chlorella protothecoides under conditions of acceptable pH and dissolved oxygen to produce a desired amount of lipid. The microalgal cells can be lysed, heat-treated, washed and dried to produce a microalgal flour that can be incorporated into a variety of products.Type: GrantFiled: January 28, 2014Date of Patent: April 23, 2019Assignee: CORBION BIOTECH, INC.Inventors: Leslie Norris, John Piechocki, Enrique Baliu, Ruchir Desai, Marie Le Ruyet, Samuel Patinier, Damien Passé, Amandine Druon
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Patent number: 10260076Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.Type: GrantFiled: February 27, 2017Date of Patent: April 16, 2019Assignee: CORBION BIOTECH, INC.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George N. Rudenko, Penelope Chua
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Patent number: 10246728Abstract: The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein.Type: GrantFiled: August 24, 2017Date of Patent: April 2, 2019Assignee: CORBION BIOTECH, INC.Inventors: David Davis, Scott Franklin, Jeffrey L. Moseley, Riyaz Bhat
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Patent number: 10167489Abstract: Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.Type: GrantFiled: June 10, 2016Date of Patent: January 1, 2019Assignee: Corbion Biotech, Inc.Inventors: Scott Franklin, Walter Rakitsky, George Rudenko, Xinhua Zhao, Felipe Arana Rodriguez, Wenhua Lu, Janice Wee
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Patent number: 10138435Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.Type: GrantFiled: June 3, 2016Date of Patent: November 27, 2018Assignee: CORBION BIOTECH, INC.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 10125382Abstract: Novel plant acyl-ACP thioesterase genes of the FatB and FatA classes and proteins encoded by these genes are disclosed. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Expression of the novel and/or mutated FATB and FATA genes is demonstrated in oleaginous microalga host cells. Furthermore, a method for producing an oil elevated in one or more of C12:0, C14:0, C16:0, C18:0 and/or C18:1 fatty acids includes transforming a cell with novel and/or mutated FATB and/or FATA genes, e.g., having an N-terminal deletion. The cells produce triglycerides with altered and useful fatty acid profiles.Type: GrantFiled: September 18, 2015Date of Patent: November 13, 2018Assignee: CORBION BIOTECH, INC.Inventors: Jason Casolari, George N. Rudenko, Scott Franklin, Xinhua Zhao
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Patent number: 10119947Abstract: The invention relates to a method for determining the organoleptic quality of a protein-rich microalgal biomass composition, comprising the determination of the content of 11 volatile organic compounds, wherein the 11 volatile organic compounds are pentanal, hexanal, 1-octen-3-ol, 2-pentylfuran, octanal, 3,5-octadien-2-ol (or 3-octen-2-one), 3,5-octadien-2-one, nonanal, 2-no-nenal, (E,E)-2,4-nonadienal and hexanoic acid.Type: GrantFiled: August 6, 2014Date of Patent: November 6, 2018Assignee: CORBION BIOTECH, INC.Inventors: Heike Jerosch, Amandine Druon, Marilyne Guillemant, Samuel Patinier
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Publication number: 20180310597Abstract: The present invention relates to noodle products comprising a high-lipid microalgal flour, and to noodle doughs suitable for preparing such noodle products. More particularly, the noodle dough comprises from 0.05% to 15% w/w of a microalgal flour comprising at least 50% of lipid by dry weight. The resulting noodle products show a reduced cooking time compared to regular noodles free of such microalgal flour.Type: ApplicationFiled: May 19, 2015Publication date: November 1, 2018Applicant: Corbion Biotech, Inc.Inventors: Goichi Ito, Yoshitaka Okubo
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Patent number: 10100341Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.Type: GrantFiled: December 18, 2015Date of Patent: October 16, 2018Assignee: CORBION BIOTECH, INC.Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walter Rakitsky, Xinhua Zhao, Riyaz Bhat
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Publication number: 20180237811Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.Type: ApplicationFiled: January 19, 2018Publication date: August 23, 2018Applicants: Corbion Biotech, Inc., Corbion Biotech, Inc.Inventors: Scott Franklin, Aravind Somanchi, George N. Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer