Abstract: A method and apparatus for microscopic imaging is provided. The method includes: illuminating the sample with illumination radiation to stimulate the detection radiation; capturing the detection radiation from the sample; with the intensity data of the detection radiation from the sample; applying the calibration algorithm to the captured image(s) to acquire the processed second image; the resolution of the processed second image is higher than the acquired first image.

Abstract: A method for controlling the growth of blue algae in a basin relates to the field of the restoration and protection of the natural ecology on the earth's surface. An ecological water treatment system was constructed for the basin with microorganisms, plants, animals, fillers and the like as main elements by building a novel ecological slope protection at the land-lake ecozone along the banks of the basin and planting eucalyptus in the basin water and/or on the bank of the basin, thereby effectively realizing the efficient nitrogen and phosphorus removal from the basin, controlling the spread of blue algae and improving the environmental water quality.

Abstract: The present application is related to a detection device and method for coronavirus and influenza virus. The device comprises a detection module (20), a signal processing circuit (30), a controller (40), a displayer (50), a digital-to-analog conversion circuit (60), and a clock (70). Noticeably, the detection module (20) comprises a sample cell, and a transistor sensor combination integrated in the sample cell and used for measuring different targets. The detection module (20) may comprise a sample cell array to realize simultaneous detection of a plurality of samples to be detected. The detection method comprises the following steps: adding a sample to be detected into a sample cell, reading an electrical signal response of each transistor sensor in the sample cell to judge whether the sample to be detected contains a virus to be detected or not. The present application belongs to the technical field of biological detection.

Abstract: A method for accelerating score-based generative models (SGM) is provided, including setting a frequency mask (R) and a space mask (A) and a target sampling iteration number (T); sampling an initial sample (x0); conducting iteration comprising steps as follows: sampling a noise term; applying a preconditioned diffusion sampling (PDS) operator (M) to the noise term and thus generate a preconditioned noise term; calculating a drift term; applying the transpose of the PDS operator (MT) and then applying the PDS operator (M) to the drift term, and thus generate a preconditioned drift term; diffusing the sample of each iteration (xt); and outputting the result.

Abstract: A method for early diagnosis of keratoconus based on multi-modal data considers a mutual relationship between both eyes using four refractive maps for corneas of the both eyes and absolute corneal elevation data, and combines the deep convolutional network method, the traditional support vector machine (SVM) method in machine learning, and the elevation map enhancement method with adjustable best-fit-sphere (BFS) to identify sensitivity and specificity of a focus and balance the sensitivity and specificity. With multi-dimensional comprehensive judgment of a keratoconus morbidity with a patient as a unit, combined with binocular data including both manual selection features and deep network learning from big data, the diagnosis method has higher robustness and accuracy.

Abstract: A dual-vector system for expressing an OTOF protein includes two nucleotide sequences, where a first nucleotide sequence includes two ITR sequences and a gene expression cassette inserted between the ITR sequences; a second nucleotide sequence includes two ITR sequences and a gene expression cassette inserted between the ITR sequences; the gene expression cassette of the first nucleotide sequence includes a promoter, an N-terminal coding sequence of OTOF, an N-terminal coding sequence of intein, and a polyA; and the gene expression cassette of the second nucleotide sequence includes a promoter, a C-terminal coding sequence of Intein, a C-terminal coding sequence of OTOF, and a polyA. The present disclosure further provides an adeno-associated virus packaged by the vector. The vector and the virus can recover hearing of bilateral ears by a unilateral ear administration in the field of deafness gene therapy large gene dual-vector delivery.

Abstract: A method for preparing (dimethylaminomethylene) malononitrile by using a micro reaction system. Cyanoacetamide, N,N-dimethylformamide and a catalyst are mixed to obtain a mixture, and the mixture and phosphorus oxychloride are simultaneously pumped into the micro reaction system that includes a micromixer and a microchannel reactor connected in series for continuous dehydration condensation. After adjusted to a target pH, the crude product is subjected to continuous liquid-liquid extraction with an organic solvent in a centrifugal extraction unit comprising a plurality of annular centrifugal extractors connected in series. The organic phase is collected to obtain the target product (dimethyl aminomethylene) malononitrile.

Abstract: Disclosed herein relates to organic synthesis, and more particularly to a method for preparing a key intermediate for the synthesis of statins. The key intermediate is 2-[(4R,6S)-6-[(benzo[d]thiazol-2-ylthio)methyl]-2,2-disubstituted-1,3-dioxan-4-yl] acetate of formula (I): where R1 is a C1-C8 alkyl group, a C3-C8 cycloalkyl group, a monosubstituted or polysubstituted aryl group, or monosubstituted or polysubstituted aralkyl group; R2 is hydrogen, or monosubstituted or polysubstituted C1-C3 alkyl group, or halogen; and R3 and R4 are each independently a C1-C5 alkyl group, a C3-C7 cycloalkyl group, a C3-C7 cycloalkenyl group, a C1-C3 alkoxy group, a C6-C10 aryl group, or C7-C12 aralkyl group. In the method, a halomethyl compound and a thiol reagent are subjected to nucleophilic substitution in an organic solvent to synthesize a thioether, which then undergoes ketal exchange reaction with a carbonyl compound (V) in the presence of an organic acid to obtain a target product.

Abstract: A method for early diagnosis of keratoconus based on multi-modal data considers a mutual relationship between both eyes using four refractive maps for corneas of the both eyes and absolute corneal elevation data, and combines the deep convolutional network method, the traditional support vector machine (SVM) method in machine learning, and the elevation map enhancement method with adjustable best-fit-sphere (BFS) to identify sensitivity and specificity of a focus and balance the sensitivity and specificity. With multi-dimensional comprehensive judgment of a keratoconus morbidity with a patient as a unit, combined with binocular data including both manual selection features and deep network learning from big data, the diagnosis method has higher robustness and accuracy.

Abstract: A system and method for gene editing by using an engineered cell are provided. The system includes the engineered cell embedded with a synthetic protein receptor and a target cell. The engineered cell contains a CRISPR/CasRx system and a sgRNA gene sequence. The synthetic protein receptor includes an extracellular target cell recognition domain, a native Notch core domain, an intramembranous hydrolyzable polypeptide and effectors. The extracellular target cell recognition domain can recognize antigen molecules on the target cell surface; and the effectors act as transcription factors for CasRx enzyme and sgRNAs. CasRx and gRNA are expressed in the engineered cell for gene editing to edit mRNA of the target cell. In this way, the application range of the engineered cell is expanded, the pertinence and specificity of gene editing are improved, the off-target effect is reduced, the collective non-specific reaction is reduced, and the safety of gene editing is improved.

Abstract: Disclosed are a furaneone glycoside compound, a pharmaceutical composition thereof, a preparation method therefor, and an application thereof. Specifically disclosed are a compound as represented by formula A-1, a pharmaceutically acceptable salt thereof or a crystal form thereof. Also disclosed is a pharmaceutical composition, which comprises the compound as represented by formula A-1, the pharmaceutically acceptable salt thereof, and a pharmaceutical adjuvant. Also disclosed is an application of the compound as represented by formula A-1, the pharmaceutically acceptable salt thereof, the crystal form thereof, or the pharmaceutical composition in the preparation of drugs. The drugs are drugs for treating inflammatory bowel diseases. The furaneone glycoside compound has a good effect of treating inflammatory bowel diseases, particularly ulcerative colitis.

Abstract: The present application is related to a detection device and method for coronavirus and influenza virus. The device comprises a detection module (20), a signal processing circuit (30), a controller (40), a displayer (50), a digital-to-analog conversion circuit (60), and a clock (70). Noticeably, the detection module (20) comprises a sample cell, and a transistor sensor combination integrated in the sample cell and used for measuring different targets. The detection module (20) may comprise a sample cell array to realize simultaneous detection of a plurality of samples to be detected. The detection method comprises the following steps: adding a sample to be detected into a sample cell, reading an electrical signal response of each transistor sensor in the sample cell to judge whether the sample to be detected contains a virus to be detected or not. The present application belongs to the technical field of biological detection.

Abstract: The present application provides a group of human immortalized B lymphocyte cell lines and use thereof, and specifically provides a combination of four closely related immortalized lymphocyte cell lines. The combination can be used as a reference substances for measuring the performance of a detection platform. When the four closely related immortalized lymphocyte cell lines are used as reference substances for epigenome, transcriptome, proteome, and metabolome, an intrinsic magnitude difference gradient can be formed to evaluate the sensitivity of histological detection.

Abstract: A computing device including a logic track including two logic-track magnetic domains separated by a logic-track domain wall, an input track arranged crossing the logic track at a first position of the logic track, and an output track arranged crossing the logic track at a second position of the logic track near the logic-track domain wall. The input track includes at least one input-track magnetic domain, and each of the at least one input-track magnetic domain includes at least one input-track storage unit configured to store binary 0 or 1. The output track includes at least one output-track magnetic domain, and each of the at least one output-track magnetic domain includes at least one output-track storage unit configured to store binary 0 or 1.

Abstract: The present disclosure proposes a modeling method and apparatus for diagnosing an ophthalmic disease based on artificial intelligence, and a storage medium. The modeling method includes: establishing a data collection of ophthalmic images and a data collection of non-image ophthalmic disease diagnosis questionnaires; training a first neural network model by employing the data collection of the ophthalmic images to obtain a first classification model; training a second classification model by employing the data collection of non-image ophthalmic disease diagnosis questionnaires; and merging the first classification model and the second classification model to obtain a target classification network model, in which, a test result outputted by the target classification network model is used as a diagnosis result of the ophthalmic disease.

Abstract: Provided in the present invention are highly stable D-configuration polypeptides DVAP and SVAP having a high binding activity to the GRP78 protein, and also provided are an L-configuration polypeptide LVAP and a DVAP-or SVAP-modified model drug and a macromolecule carrier material, and the use thereof in the construction of a tumour image and a drug-delivery system for targeted treatment.

Abstract: This disclosure relates to organic synthesis, and more particularly to a method for preparing 3-chloro-4-oxopentyl acetate using a fully continuous-flow micro-reaction system. In this method, chlorine and an acetylbutyrolactone-containing liquid are simultaneously transported to a first micro-channel reactor for continuous chlorination to obtain ?-acetyl-?-chloro-?-butyrolactone. The reaction mixture is simultaneously transported to a micro-mixer and a second micro-channel reactor together with a mixed solution of glacial acetic acid, hydrochloric acid and water, and the continuous acylation is carried out to obtain 3-chloro-4-oxopentyl acetate. After quenched with a quenching agent, the reaction mixture was subjected to extraction and separation to obtain the 3-chloro-4-oxopentyl acetate.

Abstract: A network for category-level 6D pose and size estimation, including a 3D-OCR module for 3D Orientation-Consistent Representation, a GeoReS module for Geometry-constrained Reflection Symmetry, and a MPDE module for Mirror-Paired Dimensional Estimation; wherein the 3D-OCR module and the GeoReS module are incorporated in parallel; the 3D-OCR module receives a canonical template shape including canonical category-specific keypoints; the GeoReS module receives an original input depth observation including pre-processed predicted category labels and potential masks of the target instances; the MPDE module receives the output from the GeoReS module as well as the original input depth observation; and the network outputs the estimation results based on the output of the MPDE module, the output of the 3D-OCR module, as well as the canonical template shape. Also provided are corresponding systems and methods.

Abstract: A system for image inpainting is provided, including an encoder, a decoder, and a sketch tensor space of a third-order tensor; wherein the encoder includes an improved wireframe parser and a canny detector, and a pyramid structure sub-encoder; the improved wireframe parser is used to extract line maps from an original image input to the encoder, the canny detector is used to extract edge maps from the original image, and the pyramid structure sub-encoder is used to generate the sketch tensor space based on the original image, the line maps and the edge maps; and the decoder outputs an inpainted image from the sketch tensor space. A method thereof is also provided.

Abstract: Disclosed is a non-destructive large current-readout ferroelectric single-crystal thin film memory as well as a method of preparing the ferroelectric memory and a method of operating the ferroelectric memory. The large current-readout ferroelectric single-crystal thin film memory comprises a ferroelectric storage layer, which is a ferroelectric single-crystal storage layer. The non-destructive readout ferroelectric memory has a greatly increased read current in an on-state, and moreover, the data retention performance and data endurance performance are improved.