Abstract: A flow-through conductivity sensor assembly comprises a housing (4) having a flow passage with an inlet end and an outlet end. The cross section of at least a section of the flow passage extension between the inlet and outlet ends is divided into a conductivity measuring channel (2) and a parallel by-pass channel (3) of larger cross-sectional area than the measuring channel (2).

Abstract: The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

Abstract: The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine.

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

Abstract: The invention relates to a An aseptic connector comprising a first connection unit (102; 202; 302; 402; 502) provided with a first terminal end surface (104; 204; 304; 404; 504) and a second connection unit (106; 206; 306; 406; 506) provided with a second terminal end surface (108; 208; 308; 408; 508); said first and second terminal end surfaces are each provided with at least one first and second opening (110, 112; 210, 212; 310, 312; 410, 412; 510, 512), which openings are orientated to substantially coincide when said first and second connection units are connected to each other; said first and second openings are sealed by at least one film (114; 214; 314; 414; 514) arranged on said first and second connection units, so that the contact between the film and each connection unit is aseptic; said at least one film is adapted to be mated with a corresponding film on the other connection unit when said first and second connection units are connected to each other; and said mated films are adapted to be pulled

Abstract: The invention relates to a bag locking mechanism comprising, a fixed locking means (106), provided with a first abutment surface (116), a movable locking means (108; 208), which is movable between a locking position and a releasing position, and a second abutment surface (118; 218) arranged on the movable locking means (108; 208). The fixed locking means (106) defines by its configuration, at least partly a restricted locking space (114), in which the movable locking means (108; 208) is movable between the locking and releasing position.

Abstract: The present invention relates to a device (1) for delivery of fluid, said device comprising at least two fluid ducts (12-17) of flexible material, each of which in one end can be connected to a fluid source and in the opposite end is connected to a manifold (18) having an inlet end and an outlet end, each of said fluid ducts (12-17) comprising a pinch valve (19) for closing and opening the duct. According to the invention a check valve (20) is disposed in at least one of said fluid ducts (12-17) in the end thereof connected to the manifold (18).

Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.

Abstract: The present invention relates to a mixing system comprising a vessel (1) for housing a disposable fluid mixing bag (2), which vessel (1) comprises a base (3) supporting the fluid mixing bag (2) and a sidewall (4) extending vertically upwards from the base (3). The sidewall (4) of the vessel comprises a door opening (5) which is closable by a sliding door (6). The system with the sliding door provides an easy access to the inside of the vessel (1) in a space saving manner.

Abstract: The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, O-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components.

Abstract: A method of determining multimers of a macromolecule monomer in a sample containing the macromolecule comprises the steps of (i) determining the total concentration of macromolecule in the sample, (ii) determining by a biosensor-based detection method, especially mass-sensing, the active concentration of macromolecule in the sample, wherein physical characteristics of the macromolecule monomer are used, (iii) comparing the relationship of determined active macromolecule concentration to total macromolecule concentration for the sample with a corresponding relationship for an at least substantially multimer-free macromolecule-containing sample, and (iv) from a resulting difference determining the presence of multimers in the sample. The method may be used in the purification of macromolecules, such as proteins.

Abstract: Pressure sensor unit for sealed attachment to a fluidic system comprising a mounting member, a membrane cavity extending through the mounting member with an opening in contact with the fluidic system when the sensor unit is attached thereto, a membrane formed at the distal end of the membrane cavity, and a membrane deflection sensor, wherein the membrane is separated from the mounting member by a stress insulating member arranged to isolate the membrane from stress and strain in the mounting member.

Abstract: Automated fluid handling system comprising a housing and two or more fluid handling units arranged as interchangeable modular components with an external fluidics section and an internal non fluidics section, and wherein the housing comprises a liquid handling panel with two or more of component positions for receiving said interchangeable modular components such that the external fluidics section is separated from the non fluidics section by the liquid handling panel.

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

Abstract: There is a recognized need for novel, more simplified, approaches to isolation of plasma from whole blood, as well as a need to isolate cell-free plasma fractions containing different plasma proteins. Methods are divulged for use of aqueous phase systems, formed in blood or blood containing solutions via addition of a single polymer at relatively low concentration, to effect isolation (clarification) of plasma proteins from blood cells. Methods are also divulged to replace widely used Cohn-type plasma protein fractionation which is based on sequential addition of up to 40% (v/v) ethanol and other precipitants, with simple sequential addition of a polyacid. The latter results in isolation of plasma protein fractions (i.e. fibrinogen, immunoglobulin, albumin) in sequence similar to that obtained with Cohn Fractionation and therefore may be suitable for use to reduce solvent use and solvent-related process complications in existing plasma protein purification processes.

Abstract: Disclosed is piston pump (10) and piston assembly (30) for such a pump. The piston pump includes a cylinder (20) having an inner wall surface (22) formed around an axis A of the cylinder, and a piston assembly (30) for sliding along said cylinder axis, said piston assembly includes a slideable flexible seal (34) in generally sealing contact with said inner wall surface, and a seal mounting (44), said seal mounting having a plurality of circumferentially arranged members (37) moveable radially of said axis to increase or decrease the size of the seal.

Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.

Abstract: The present invention relates to a method for detection of binding or interaction events between a binding agent and its corresponding analyte (such as an antibody and an antigen) in which a signal is detected which is substantially more amplified and thus easier to detect than in prior art systems. The method comprises simultaneous but separate addition of a first enhancement reagent having affinity for said analyte and a second enhancement reagent having affinity for the first enhancement reagent wherein the first enhancement reagent binds to the analyte and the second enhancement reagent binds to the first enhancement reagent, and, wherein the first and second enhancement reagents have more than one binding site so that they are able to bind to each other to thereby amplify a detectable signal from the binding event.

Abstract: The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, O-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components.