Abstract: Methods, systems, and apparatus to monitor component status in a bioprocessing environment are disclosed and described. Certain examples provide a sensor device for a disposable bioprocessing component. The example sensor device includes a first portion affixed to the component, the first portion configured to provide an identifier associated with the component. The example sensor device also includes a second portion configured to provide a status indication based on a state of the component. The example sensor device is configured to transmit the identifier and status indication to a control computer associated with a bioprocessing platform including the component.

Abstract: A rotary valve comprising a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face, the rotor is rotatably movable to a plurality of rotor positions about a rotational axis relative to the inner stator face, the stator comprises a plurality of connection ports each being in fluidic contact with a corresponding valve orifice at the inner stator face and the rotor comprises two or more interconnection paths for selective fluidic interconnection of said valve orifices with respect to the rotor position, wherein the stator comprises, an inlet port, an outlet port, a component feed port, a component return port, and wherein the interconnection paths in the rotor are arranged to: —in a first rotor position interconnect the inlet port with the outlet port, —in a second rotor position interconnect the inlet port with the component feed port and the component return port with the outlet port, —in a third rotor position interconnect the inlet port w

Abstract: An attachment arrangement for providing a sensor in a flexible bag, said attachment arrangement comprising: a sensor holding part adapted to be integrated with the flexible bag and comprising first locking part protruding out form the flexible bag, the sensor holding part holding an optical sensor inside the flexible bag; an adaptor part provided outside the flexible bag and adapted to hold an optical fiber, the adaptor part comprising second locking part adapted to mate with the first locking part to lock the first locking part and the second locking part together such that the optical fiber can communicate with the optical sensor inside the bag.

Abstract: A chromatography column frame comprising at least two legs, a support arrangement connected to said at least two legs, and a holder connected to said support arrangement. Said holder is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction.

Abstract: Methods, systems, and apparatus to monitor component status in a bioprocessing environment are disclosed and described. Certain examples provide a sensor device for a disposable bioprocessing component. The example sensor device includes a first portion affixed to the component, the first portion configured to provide an identifier associated with the component. The example sensor device also includes a second portion configured to provide a status indication based on a state of the component. The example sensor device is configured to transmit the identifier and status indication to a control computer associated with a bioproces sing platform including the component.

Abstract: An inflatable bioreactor bag for cell cultivation, which comprising a top and a bottom sheet of flexible material, joined together to form two end edges and two side edges, wherein one baffle or a plurality of baffles extend from the bottom sheet in a region where the shortest distance to any one of the two end edges is higher than about one fourth of the shortest distance between the two end edges.

Abstract: A container comprising a first material and a second material, the first material being a flexible material and the second material being a rigid or semi-rigid material and at least partially defining an interior compartment for keeping a fluid inside the container characterized in that the flexible material and/or the semi-rigid or rigid material have thermoplastic properties and wherein the flexible material and the semi-rigid or rigid material are joined together by a seal or joint which comprises a heat seal and an adhesive seal.

Abstract: The invention relates to a method for making a laminated microfluidics with membrane valves. The method comprises (a) generating a membrane valve assembly comprising (1) laminating three plastic foils together, the top foil comprising one through hole for each valve, the middle foil has a slit pattern connecting each valve hole of the top foil, and the bottom foil has one through hole for each valve and one additional hole entering the slit pattern; (2) placing the laminated foil, bottom foil down on a mould half having a smooth mould surface with one or more perpendicularly protruding cylinders that concentrically fit within the through holes of the laminated foil yet leave room for a membrane to form, and clamping a second mould half to close the mould; (3) injecting and allowing to cure a liquid rubber through a funnel associated with the bottom mould half and the additional hole on the bottom foil layer; and (b) laminating the membrane valve assembly and a flow path.

Abstract: A rotary valve comprising a stator and a rotor, wherein the stator comprises at least three primary connection ports and at least three secondary connection ports, and wherein rotor interconnection paths are arranged to in the different rotor positions interconnect the primary connection ports with the secondary connection ports such that all of at least three secondary connection ports can be connected one at the time to each of at least three primary connection port by rotating the rotor into the different rotor positions. A chromatography system comprising at least three chromatography columns and a column inlet rotary valve, a column outlet rotary valve and a feed recirculation flow path.

Abstract: A method of determining aggregates of a macromolecule monomer in a fluid containing the macromolecule, comprises the steps of: contacting a sample of the fluid with a sensing surface of an interaction analysis sensor, wherein the sensing surface is capable of specific binding interaction with the macromolecule, determining at least one kinetic parameter for the interaction of the fluid sample with the sensing surface, comparing the determined kinetic parameter or parameters with that or those determined for at least one fluid sample having a known fraction or fractions of aggregates of the macromolecule, and determining therefrom the fraction of macromolecule in the sample that is in the form of aggregate or aggregates.

Abstract: The invention relates to a system and method for mixing two or more solutions. Provided is an integrated system for generating a homogeneous solution from two or more input solutions, comprising: a mixer chamber containing a magnetic stir bar; a means for rotating the magnetic stir bar; and an user interface for input a chosen flow rate. The mixer chamber comprises a mixer chamber body including a movable upper part which defines a mixer chamber volume; one or more solution inlets; an solution outlet; the magnetic stir bar; and a means for adjusting the mixer chamber volume. In response to the input flow rate, the mixing chamber volume is adjusted to an optimal volume, by the movement of the movable upper part, controlled by the means for adjusting the mixer chamber volume. Also provided are methods of using the system to achieve homogeneous mixing of two or more solutions.

Abstract: A method of determining the relative component proportions of at least one each of: a buffer; an acid or a base; a solvent; and optionally a salt, for providing a liquid mixture of pre-defined pH and ionic strength, wherein the relative component proportions are determined using the equation of Debye-Hückel, wherein the ion size parameter a in the Debye-Hückel equation is determined as the weighted mean ion size of all species contributing to the ionic strength of the liquid mixture, and wherein the ionic strength of each species is used as weighting parameter. The present method is also applicable in a method of providing a liquid mixture. Further there is provided a buffer preparation device.

Abstract: The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins.

Abstract: The present invention relates to a bioreactor and a method for operating a bioreactor which comprises a chamber (100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter (210; 212; 214). The filter assembly (200) is disposed in the chamber (100) and the filter assembly (200) is free to move within the chamber (100). The filter assembly (200) comprises means for coupling a harvesting flow through said filter (210; 212; 214) and means for coupling a feed flow through said filter in an opposite direction compared to the harvesting flow. Through this arrangement, the clogging prevention of the perfusion filter can be improved.

Abstract: The present invention relates to a method for production of a chromatography material. More closely, the invention relates to a method for production of a reverse phase chromatography (RPC) material comprising the following steps: introduction of unsaturated groups onto porous carbohydrate particles and grafting of styrenic monomers on said particles comprising an unsaturated group.

Abstract: An optical sensor holding device adapted to be integrated with a flexible bag, said device comprising a bag attachment part made from a material that can be welded to the flexible bag; a sensor attachment part made from a material to which an optical sensor spot can be attached, wherein the sensor attachment part is forced into an opening in the bag attachment part that is slightly smaller than the sensor attachment part such that the sensor attachment part is kept firmly in place and leakage is minimized.

Abstract: The invention relates to a chromatography column assembly that offers an affordable self-packed column for performing chromatographic separation. The chromatography column assembly comprises a column tube having a first and a second opening at substantially opposite ends, and an end cap of either a snap fit design or a bolted design. The column assembly may further include an adaptor shaft inserted into the column through the end cap for achieving a desired bed volume. The column assembly may also include a second end cap enclosing the second opening, and an optional adaptor shaft may be inserted into the column through the second end cap, enabling further adjustment of the bed volume.