Patents Assigned to Gen9, Inc.
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Patent number: 10240194Abstract: Methods of obtaining sequence information about target polynucleotide having a predefined sequence are disclosed. The methods include sequencing by ligation and sequencing by polymerase.Type: GrantFiled: May 13, 2011Date of Patent: March 26, 2019Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler
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Patent number: 10207240Abstract: Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence.Type: GrantFiled: November 3, 2010Date of Patent: February 19, 2019Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Larry Li-Yang Chu, Senthil Ramu
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Patent number: 10202608Abstract: Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits.Type: GrantFiled: September 30, 2011Date of Patent: February 12, 2019Assignee: Gen9, Inc.Inventor: William J. Blake
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Publication number: 20190010530Abstract: Aspects of the disclosure relate to compositions and methods for polynucleotide assembly. In some embodiments, a terminator oligonucleotide is provided.Type: ApplicationFiled: December 21, 2016Publication date: January 10, 2019Applicant: Gen9, Inc.Inventor: Ishtiaq Saaem
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Publication number: 20180355353Abstract: Artificial transcription terminators and their use are provided herein. In one aspect, a non-naturally occurring nucleic acid sequence can comprise a Y-X-Z stem-loop, wherein: Y is a nucleotide sequence of 10 to 30 nucleotides in length; X is a nucleotide sequence of 3 to 12 nucleotides in length, each nucleotide therein not base pairing with any other nucleotide within X; and Z is a nucleotide sequence of 10 to 50 nucleotides in length and having at least 70% complementarity to Y.Type: ApplicationFiled: November 29, 2016Publication date: December 13, 2018Applicant: Gen9, Inc.Inventor: Ishtiaq E. Saaem
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Patent number: 10081807Abstract: Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.Type: GrantFiled: April 24, 2013Date of Patent: September 25, 2018Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Martin J. Goldberg, Li-yun A. Kung, Daniel Schindler, Michael E. Hudson
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Patent number: 10053719Abstract: Aspects of the invention relate to methods, compositions for synthesizing high fidelity oligonucleotides.Type: GrantFiled: March 13, 2014Date of Patent: August 21, 2018Assignee: Gen9, Inc.Inventors: Ishtiaq E. Saaem, Michael E. Hudson, Stephen F. Archer, Sophea Sann-Rorth, Ellen J. Sanchez, Joseph Jacobson
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Patent number: 10032005Abstract: Embodiments of the invention provide systems and methods to provide safe, secure and accurate point-to-point delivery of prescription and non-prescription drugs in the long-term home care or ambulatory care environment. More specifically, embodiments of the present invention provide for a low-cost, easy-to-use system comprised of a secure drug dispensing unit and medication enclosure combined with wireless connectivity and software based on smart mobile phone technology. Such systems and methods, referred to herein as a Secure, Control, and Enhance Medication Adherence (SCEMA) system, can mitigate the aforementioned risks associated with the use of prescription and non-prescription drugs. These risks can be significantly reduced for the elderly in the homecare environment, as well as the general public, without disrupting, or a significantly increasing the cost, to the existing prescription and non-prescription drug distribution infrastructure.Type: GrantFiled: May 10, 2016Date of Patent: July 24, 2018Assignee: GEN-9, INC.Inventor: Mark A. Fauci
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Patent number: 9968902Abstract: Certain aspects of the present invention provide devices and methods for preparing oligonucleotides and for assembling nucleic acid molecules using microfluidic devices.Type: GrantFiled: November 20, 2015Date of Patent: May 15, 2018Assignee: Gen9, Inc.Inventor: Larry Li-Yang Chu
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Patent number: 9938553Abstract: Disclosed are devices and methods to synthesize polynucleotides and libraries of polynucleotides such as libraries of oligonucleotides. In exemplary embodiments, the device includes a support having a plurality of features. Each feature contains a plurality of oligonucleotides. Within each feature, each of the plurality of oligonucleotides includes an identical predetermined subunit sequence of X nucleosides and a degenerate sequence of Y nucleosides. A predetermined combination of a subset of the features can be used to produce a polynucleotide having a predetermined sequence of Z nucleosides.Type: GrantFiled: April 8, 2016Date of Patent: April 10, 2018Assignee: Gen9, Inc.Inventor: Joseph Jacobson
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Patent number: 9925510Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: GrantFiled: November 20, 2015Date of Patent: March 27, 2018Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Larry Li-Yang Chu
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Patent number: 9856471Abstract: Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.Type: GrantFiled: June 27, 2014Date of Patent: January 2, 2018Assignee: Gen9, Inc.Inventors: Joseph Jacobson, George Church, Larry Li-Yang Chu
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Publication number: 20160253479Abstract: Embodiments of the invention provide systems and methods to provide safe, secure and accurate point-to-point delivery of prescription and non-prescription drugs in the long-term home care or ambulatory care environment. More specifically, embodiments of the present invention provide for a low-cost, easy-to-use system comprised of a secure drug dispensing unit and medication enclosure combined with wireless connectivity and software based on smart mobile phone technology. Such systems and methods, referred to herein as a Secure, Control, and Enhance Medication Adherence (SCEMA) system, can mitigate the aforementioned risks associated with the use of prescription and non-prescription drugs. These risks can be significantly reduced for the elderly in the homecare environment, as well as the general public, without disrupting, or a significantly increasing the cost, to the existing prescription and non-prescription drug distribution infrastructure.Type: ApplicationFiled: May 10, 2016Publication date: September 1, 2016Applicant: GEN-9, INC.Inventor: Mark A. Fauci
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Patent number: 9422600Abstract: Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: GrantFiled: June 20, 2011Date of Patent: August 23, 2016Assignee: Gen9, Inc.Inventors: Senthil Ramu, Joseph Jacobson
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Patent number: 9388407Abstract: Disclosed are devices and methods to synthesize polynucleotides and libraries of polynucleotides such as libraries of oligonucleotides. In exemplary embodiments, the device includes a support having a plurality of features. Each feature contains a plurality of oligonucleotides. Within each feature, each of the plurality of oligonucleotides includes an identical predetermined subunit sequence of X nucleosides and a degenerate sequence of Y nucleosides. A predetermined combination of a subset of the features can be used to produce a polynucleotide having a predetermined sequence of Z nucleosides.Type: GrantFiled: March 31, 2014Date of Patent: July 12, 2016Assignee: Gen9, Inc.Inventor: Joseph Jacobson
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Patent number: 9361431Abstract: Embodiments of the invention provide systems and methods to provide safe, secure and accurate point-to-point delivery of prescription and non-prescription drugs in the long-term home care or ambulatory care environment. More specifically, embodiments of the present invention provide for a low-cost, easy-to-use system comprised of a secure drug dispensing unit and medication enclosure combined with wireless connectivity and software based on smart mobile phone technology. Such systems and methods, referred to herein as a Secure, Control, and Enhance Medication Adherence (SCEMA) system, can mitigate the aforementioned risks associated with the use of prescription and non-prescription drugs. These risks can be significantly reduced for the elderly in the homecare environment, as well as the general public, without disrupting, or a significantly increasing the cost, to the existing prescription and non-prescription drug distribution infrastructure.Type: GrantFiled: April 1, 2013Date of Patent: June 7, 2016Assignee: GEN-9, INC.Inventor: Mark A. Fauci
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Patent number: 9295965Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: GrantFiled: November 10, 2011Date of Patent: March 29, 2016Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Mike Hudson
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Publication number: 20150368687Abstract: Aspects of the invention relate to methods, compositions for synthesizing high fidelity oligonucleotides.Type: ApplicationFiled: March 13, 2014Publication date: December 24, 2015Applicant: Gen9, Inc.Inventors: Ishtiaq E. Saaem, Michael E. Hudson, Stephen F. Archer, Sophea Sann-Rorth, Ellen J. Fanti, Joseph Jacobson
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Patent number: 9216414Abstract: Certain aspects of the present invention provide devices and methods for preparing oligonucleotides and for assembling nucleic acid molecules using microfluidic devices.Type: GrantFiled: November 19, 2010Date of Patent: December 22, 2015Assignee: Gen9, Inc.Inventor: Larry Li-Yang Chu
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Patent number: 9217144Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: GrantFiled: January 6, 2011Date of Patent: December 22, 2015Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Larry Li-Yang Chu