Patents Assigned to Gen9, Inc.
-
Patent number: 9187777Abstract: Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.Type: GrantFiled: May 26, 2011Date of Patent: November 17, 2015Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Senthil Ramu, Daniel Schindler
-
Patent number: 9150853Abstract: Disclosed are methods, compositions and devices for screening a protein library for proteins having a desired activity, such as capable of catalyzing the formation of a bond between two reactants. In an exemplary embodiments, a plurality of proteins are expressed in vitro from a plurality of nucleic acids, the plurality of proteins are exposed with two single stranded oligonucleotides having complementary sequences, each oligonucleotide having a reactant and a fluorophore, the fluorescence of the protein-reactant-oligonucleotide-fluorophore complexes is detected and the complexes showing detectable fluorescence energy transfer are isolated, thereby isolating proteins having the desired enzymatic activity.Type: GrantFiled: March 13, 2013Date of Patent: October 6, 2015Assignee: Gen9, Inc.Inventors: Michael E. Hudson, Joseph Jacobson
-
Publication number: 20150203839Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.Type: ApplicationFiled: August 23, 2012Publication date: July 23, 2015Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler, Scott S. Lawton
-
Patent number: 9051666Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.Type: GrantFiled: September 14, 2012Date of Patent: June 9, 2015Assignee: Gen9, Inc.Inventor: Andrew V. Oleinikov
-
Patent number: 9023601Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.Type: GrantFiled: September 30, 2011Date of Patent: May 5, 2015Assignee: Gen9, Inc.Inventor: Andrew V. Oleinikov
-
Publication number: 20150065393Abstract: Disclosed are devices and methods to synthesize polynucleotides and libraries of polynucleotides such as libraries of oligonucleotides. In exemplary embodiments, the device includes a support having a plurality of features. Each feature contains a plurality of oligonucleotides. Within each feature, each of the plurality of oligonucleotides includes an identical predetermined subunit sequence of X nucleosides and a degenerate sequence of Y nucleosides. A predetermined combination of a subset of the features can be used to produce a polynucleotide having a predetermined sequence of Z nucleosides.Type: ApplicationFiled: March 31, 2014Publication date: March 5, 2015Applicant: Gen9, Inc.Inventor: Joseph Jacobson
-
Patent number: 8808986Abstract: Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.Type: GrantFiled: August 27, 2009Date of Patent: August 19, 2014Assignee: Gen9, Inc.Inventors: Joseph Jacobson, George Church, Larry Li-Yang Chu
-
Patent number: 8788193Abstract: Embodiments of the invention provide systems and methods for tracking a mobile device using sensors within the device and without using external signals to determine location, velocity, or heading. According to one embodiment, locating and tracking a mobile device can comprise reading sensor data from each of a plurality of sensors within the mobile device. The sensors can provide the sensor data without relying on a signal from a device external to the mobile device. For example, the plurality of sensors can comprise a compass, a gyroscope, and an accelerometer. Motion, velocity, and heading of the mobile device can be determined based on the sensor data.Type: GrantFiled: October 16, 2012Date of Patent: July 22, 2014Assignee: GEN-9, Inc.Inventors: Mark A. Fauci, Yinghao Ma, Craig Poppe, Thung-Han Hee, Rahul Agrawal
-
Publication number: 20140141982Abstract: Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.Type: ApplicationFiled: April 24, 2013Publication date: May 22, 2014Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Martin J. Goldberg, Li-yun A. Kung, Daniel Schindler, Michael E. Hudson
-
Patent number: 8716467Abstract: Disclosed are devices and methods to synthesize polynucleotides and libraries of polynucleotides such as libraries of oligonucleotides. In exemplary embodiments, the device includes a support having a plurality of features. Each feature contains a plurality of oligonucleotides. Within each feature, each of the plurality of oligonucleotides includes an identical predetermined subunit sequence of X nucleosides and a degenerate sequence of Y nucleosides. A predetermined combination of a subset of the features can be used to produce a polynucleotide having a predetermined sequence of Z nucleosides.Type: GrantFiled: March 2, 2011Date of Patent: May 6, 2014Assignee: Gen9, Inc.Inventor: Joseph Jacobson
-
Publication number: 20130309725Abstract: Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.Type: ApplicationFiled: May 26, 2011Publication date: November 21, 2013Applicant: GEN9, INC.Inventors: Joseph Jacobson, Senthil Ramu, Daniel Schindler
-
Publication number: 20130296192Abstract: Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.Type: ApplicationFiled: November 10, 2011Publication date: November 7, 2013Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Michael E. Hudson, Larry Li-Yang Chu
-
Publication number: 20130296194Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: ApplicationFiled: November 10, 2011Publication date: November 7, 2013Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Mike Hudson
-
Publication number: 20130261794Abstract: Embodiments of the invention provide systems and methods to provide safe, secure and accurate point-to-point delivery of prescription and non-prescription drugs in the long-term home care or ambulatory care environment. More specifically, embodiments of the present invention provide for a low-cost, easy-to-use system comprised of a secure drug dispensing unit and medication enclosure combined with wireless connectivity and software based on smart mobile phone technology. Such systems and methods, referred to herein as a Secure, Control, and Enhance Medication Adherence (SCEMA) system, can mitigate the aforementioned risks associated with the use of prescription and non-prescription drugs. These risks can be significantly reduced for the elderly in the homecare environment, as well as the general public, without disrupting, or a significantly increasing the cost, to the existing prescription and non-prescription drug distribution infrastructure.Type: ApplicationFiled: April 1, 2013Publication date: October 3, 2013Applicant: GEN-9, INC.Inventor: MARK A. FAUCI
-
Publication number: 20130252849Abstract: Disclosed are methods, compositions and devices for screening a protein library for proteins having a desired activity, such as capable of catalyzing the formation of a bond between two reactants. In an exemplary embodiments, a plurality of proteins are expressed in vitro from a plurality of nucleic acids, the plurality of proteins are exposed with two single stranded oligonucleotides having complementary sequences, each oligonucleotide having a reactant and a fluorophore, the fluorescence of the protein-reactant-oligonucleotide-fluorophore complexes is detected and the complexes showing detectable fluorescence energy transfer are isolated, thereby isolating proteins having the desired enzymatic activity.Type: ApplicationFiled: March 13, 2013Publication date: September 26, 2013Applicant: Gen9, Inc.Inventors: Michael E. Hudson, Joseph Jacobson
-
Publication number: 20130244884Abstract: Methods of obtaining sequence information about target polynucleotide having a predefined sequence are disclosed. The methods include sequencing by ligation and sequencing by polymerase.Type: ApplicationFiled: May 13, 2011Publication date: September 19, 2013Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler
-
Publication number: 20130096817Abstract: Embodiments of the invention provide systems and methods for tracking a mobile device using sensors within the device and without using external signals to determine location, velocity, or heading. According to one embodiment, locating and tracking a mobile device can comprise reading sensor data from each of a plurality of sensors within the mobile device. The sensors can provide the sensor data without relying on a signal from a device external to the mobile device. For example, the plurality of sensors can comprise a compass, a gyroscope, and an accelerometer. Motion, velocity, and heading of the mobile device can be determined based on the sensor data.Type: ApplicationFiled: October 16, 2012Publication date: April 18, 2013Applicant: GEN-9, INC.Inventor: GEN-9, INC.
-
Publication number: 20130059761Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: ApplicationFiled: January 6, 2011Publication date: March 7, 2013Applicant: Gen 9, Inc.Inventors: Joseph Jacobson, Larry Li-Yang Chu
-
Publication number: 20130059296Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.Type: ApplicationFiled: August 23, 2012Publication date: March 7, 2013Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler, Scott S. Lawton
-
Publication number: 20130017977Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.Type: ApplicationFiled: September 14, 2012Publication date: January 17, 2013Applicant: Gen9, Inc.Inventor: Andrew V. Oleinikov