Patents Assigned to Invitrogen Corporation
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Patent number: 8227260Abstract: Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.Type: GrantFiled: June 23, 2008Date of Patent: July 24, 2012Assignee: Invitrogen CorporationInventors: Juan Yguerabide, Evangelina E. Yguerabide, David E. Kohne, Jeffrey T. Jackson
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Patent number: 8192932Abstract: The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5? end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5? end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.Type: GrantFiled: February 12, 2010Date of Patent: June 5, 2012Assignees: Sloan-Kettering Institute For Cancer Research, Invitrogen CorporationInventors: Stewart Shuman, JoAnn Sekiguchi, John Comiskey, Joseph Fernandez, James Hoeffler, Robert Marcil
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Publication number: 20110306098Abstract: The present invention relates generally to recombinant genetic technology. More particularly, the present invention relates to compositions and methods for use in selection and isolation of nucleic acid molecules. The invention further relates to methods for the preparation of individual nucleic acid molecules and populations of nucleic acid molecules, as well as nucleic acid molecules produced by these methods. The invention also relates to screening and/or selection methods for identifying and/or isolating nucleic acid molecules which have one or more common features (e.g., characteristics, activities, etc) and populations of nucleic acid molecules which share one or more features.Type: ApplicationFiled: May 5, 2011Publication date: December 15, 2011Applicant: Invitrogen CorporationInventors: Michael A. Brasch, David Cheo, Xiao Li, Dominic Esposito, Devon R.N. Byrd
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Publication number: 20110275541Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.Type: ApplicationFiled: January 8, 2008Publication date: November 10, 2011Applicant: INVITROGEN CORPORATIONInventors: David Cheo, Michael A. Brasch, Gary F. Temple, James L. Hartley, Devon R.N. Byrd
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Publication number: 20110143350Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.Type: ApplicationFiled: September 29, 2008Publication date: June 16, 2011Applicant: INVITROGEN CORPORATIONInventors: IRINA NAZARENKO, AYOUB RASHTCHIAN
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Publication number: 20110046201Abstract: The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.Type: ApplicationFiled: April 30, 2008Publication date: February 24, 2011Applicant: INVITROGEN CORPORATIONInventors: Jonathan D. Chesnut, Knut R. Madden, Miroslav Dudas, Louis Leong, Adam N. Harris
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Publication number: 20100291702Abstract: The present invention provides for functionalized fluorescent nanocrystal compositions and methods for making these compositions. The compositions are fluorescent nanocrystals coated with at least one material. The coating material has chemical compounds or ligands with functional groups or moieties with conjugated electrons and moieties for imparting solubility to coated fluorescent nanocrystals in aqueous solutions. The coating material provides for functionalized fluorescent nanocrystal compositions which are water soluble, chemically stable, and emit light with a high quantum yield and/or luminescence efficiency when excited with light. The coating material may also have chemical compounds or ligands with moieties for bonding to target molecules and cells as well as moieties for cross-linking the coating.Type: ApplicationFiled: March 13, 2007Publication date: November 18, 2010Applicant: INVITROGEN CORPORATIONInventor: Imad Naasani
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Publication number: 20100285490Abstract: The present invention relates to, in part, methods, reagents and apparatuses for the detection of agents. The present invention also relates, in part, to compositions including, but not limited to, flow cells, assay chambers, reagent reservoir delivery units and devices for holding an assay chamber. The present invention also provides various components and combinations of components for various detection apparatuses. The present invention also relates to a portable agent detection apparatus that can be used in the field or at a point of care and is not limited to specialized laboratories or limited to use by highly skilled users.Type: ApplicationFiled: December 28, 2007Publication date: November 11, 2010Applicant: INVITROGEN CORPORATIONInventors: Robert Dees, Dale Dembrow, Brent Henry, Espir Kahatt, James Meegan, Timothy Powers, Steven Roman, Jeffrey Rossio
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Publication number: 20100267128Abstract: The present invention relates generally to recombinant genetic technology. More particularly, the present invention relates to compositions and methods for use in selection and isolation of nucleic acid molecules. The invention further relates to methods for the preparation of individual nucleic acid molecules and populations of nucleic acid molecules, as well as nucleic acid molecules produced by these methods. The invention also relates to screening and/or selection methods for identifying and/or isolating nucleic acid molecules which have one or more common features (e.g., characteristics, activities, etc) and populations of nucleic acid molecules which share one or more features.Type: ApplicationFiled: May 14, 2008Publication date: October 21, 2010Applicant: INVITROGEN CORPORATIONInventors: Michael A. Brasch, David Cheo, Xiao Li, Dominic Esposito, Devon R. N. Byrd
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Publication number: 20100255601Abstract: The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.Type: ApplicationFiled: January 24, 2007Publication date: October 7, 2010Applicant: INVITROGEN CORPORATIONInventors: Matthew P. BEAUDET, David C. HAGEN, Jill HENDRICKSON, Rich B. MEYER
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Publication number: 20100233726Abstract: This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.Type: ApplicationFiled: November 4, 2009Publication date: September 16, 2010Applicants: The Regents of the University of California, Invitrogen CorporationInventors: Roger Y. Tsien, Roger Heim, Andrew Cubitt
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Publication number: 20100136695Abstract: Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.Type: ApplicationFiled: April 3, 2008Publication date: June 3, 2010Applicant: INVITROGEN CORPORATIONInventor: Tod M. Woolf
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Publication number: 20100081579Abstract: A method for preserving a sample is described. A method for preserving a sample device such as microarrays, slides and membranes is described. The preservation is achieved by applying a coating composition to a sample or sample device, and curing the coating composition. Candidate coating materials for forming the coating compositions are described. Preferably, the coating composition is an optically clear, solidifying solution. Also described are preservation kits which provide materials and instructions for the preservation of sample devices. Calibration devices are also described.Type: ApplicationFiled: May 20, 2009Publication date: April 1, 2010Applicant: INVITROGEN CORPORATIONInventors: Paul BUSHWAY, Laurence Warden, Todd Peterson, David E. Kohne, Juan Yguerabide
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Publication number: 20100075382Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).Type: ApplicationFiled: December 1, 2008Publication date: March 25, 2010Applicant: INVITROGEN CORPORATIONInventors: Jun E. Lee, Robert J. Potter, David Mandelman
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Publication number: 20100069259Abstract: A method for archiving sample devices such as microarray slides and membranes is described using an optically clear, solidifying solution. Also described are related methods and kits.Type: ApplicationFiled: June 19, 2009Publication date: March 18, 2010Applicant: INVITROGEN CORPORATIONInventors: Paul BUSHWAY, Laurence WARDEN, Todd PETERSON, David E. KOHNE, Juan YGUERABIDE
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Publication number: 20100068748Abstract: The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample.Type: ApplicationFiled: December 20, 2006Publication date: March 18, 2010Applicant: INVITROGEN CORPORATIONInventors: Kyle Gee, Vladimir Martin
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Publication number: 20100015601Abstract: The present disclosure describes a method and apparatus for collecting samples of particles from air or other gases at one or more monitored locations, and identifying in real-time whether biological agents, such as bacterial or viral pathogens, are present in the samples. The apparatus preferably uses a liquid-assisted collector to collect the sample of particles, which are suspended in a liquid that contains dyes that detect the presence of nucleic acids. An integrated detector with a light source and a light detector detects whether there is a change in the fluorescence of the liquid, which indicates the presence of a biological agent in the sample.Type: ApplicationFiled: November 23, 2005Publication date: January 21, 2010Applicant: INVITROGEN CORPORATIONInventors: James Gilmore, James Meegan
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Publication number: 20090326208Abstract: A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.Type: ApplicationFiled: April 30, 2008Publication date: December 31, 2009Applicant: INVITROGEN CORPORATIONInventors: John Carrino, James Fan, Robert P. Bennett, Jonathan D. Chesnut, Martin A. Gleeson, Knut R. Madden
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Publication number: 20090311193Abstract: Provided are reagents and methods for non-invasive in vivo imaging wherein the reagents comprise targeted carrier molecules conjugated to a NIR reporter molecule. In one aspect the targeted carrier molecule is an antibody, or fragment thereof that has specificity for an antigen in a living body, animal or human. In one embodiment the antibodies are anti-cancer/tumor marker antibodies, organ specific antibodies, tissue specific antibodies, cell type specific antibodies, cell surface specific antibodies, anti-viral antibodies, anti-bacterial antibodies and anti-pathogenic antibodies. The NIR reporter molecules are any fluorescent reporter molecule compatible with in vivo imaging and generally having an excitation wavelength of at least 580 nm.Type: ApplicationFiled: March 23, 2007Publication date: December 17, 2009Applicant: Invitrogen CorporationInventors: John Mauro, Thomas Steinberg, Julie Nyhus
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Publication number: 20090264304Abstract: The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.Type: ApplicationFiled: September 6, 2007Publication date: October 22, 2009Applicant: INVITROGEN CORPORATIONInventors: Wu-Bo Li, Paul E. Nisson, Joel Jessee