Patents Assigned to Invitrogen Corporation
-
Patent number: 7501288Abstract: A method and apparatus for interrogating a target having a plurality of plasmon resonant particles (PREs) distributed in the target are disclosed. In the method, a field containing the target is illuminated, and one or more spectral emission characteristics of the light-scattering particles in the field are detected. From this data, an image of positions and spectral characteristic values in the field is constructed, allowing PREs with a selected spectral signature to be discriminated from other light-scattering entities, to provide information about the field. Also disclosed are a novel PRE composition for use in practicing the method, and a variety of diagnostic applications of the method.Type: GrantFiled: December 18, 2000Date of Patent: March 10, 2009Assignee: Invitrogen CorporationInventors: Sheldon Schultz, David A. Schultz, David R. Smith, Jack J. Mock, Thomas J. Silva
-
Patent number: 7501542Abstract: The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.Type: GrantFiled: January 21, 2004Date of Patent: March 10, 2009Assignee: Invitrogen CorporationInventors: Alberto Haces, Valentina C. Ciccarone
-
Publication number: 20090061516Abstract: The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of tile present invention can be used for growth of animal cells for virus production.Type: ApplicationFiled: September 11, 2008Publication date: March 5, 2009Applicant: INVITROGEN CORPORATIONInventors: Paul Price, Steven Gorfien, Douglas Danner, Mark Plavsic
-
Publication number: 20090053691Abstract: The present invention relates to cells, methods, compositions and kits for determining the concentration of virus in a stock, i.e., determining the titer of a viral stock.Type: ApplicationFiled: July 21, 2008Publication date: February 26, 2009Applicant: INVITROGEN CORPORATIONInventors: Steven Peppers, Steven H. Harwood, Robert P. Bennett
-
Publication number: 20090047692Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a ?-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a ?-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a ?-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a ?-lactmase or instructions for using the components in an assay.Type: ApplicationFiled: April 7, 2008Publication date: February 19, 2009Applicant: INVITROGEN CORPORATIONInventors: Schuyler Boon CORRY, William Louis Downey, Brian Filanoski, Kyle Richard Gee, I. Lawrence Greenfield, James David Hirsch, Iain Johnson, Aleksey Rukavishnikov
-
Publication number: 20090047683Abstract: Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, ? has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.Type: ApplicationFiled: September 29, 2008Publication date: February 19, 2009Applicant: INVITROGEN CORPORATIONInventors: Jason Alfred Dallwig, David Carl Hagen, Shih-Jung Huang, Gerald Alan Thomas, Stephen Yue
-
Publication number: 20090035868Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.Type: ApplicationFiled: May 16, 2008Publication date: February 5, 2009Applicant: INVITROGEN CORPORATIONInventors: Thomas Diller, George Hanson, Bethany Sutton, Timothy Updyke
-
Publication number: 20090035810Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.Type: ApplicationFiled: May 29, 2008Publication date: February 5, 2009Applicant: INVITROGEN CORPORATIONInventors: Wai-Yee Leung, Ching-Ying Cheung, Stephen Yue
-
Publication number: 20090037293Abstract: The invention relates to systems and methods for marketing and using products such as liquid materials, especially liquid reagents for use in microbiological and cellular biological laboratory settings include the use of unique color and simple numeric or alphanumeric identifiers to quickly and easily identify any product from a catalog list of products. Methods of marketing, advertising and producing such products are also disclosed. Particular embodiments include products, product packaging and product labeling. The invention also relates to collars and sleeves for containers, as well as related methods of use.Type: ApplicationFiled: June 9, 2008Publication date: February 5, 2009Applicant: INVITROGEN CORPORATIONInventors: Axel Unger, Elgin Meike Topfer, Nicole Busch, Tom Hynek, Fredrik Johan Aidehag, Todd Pelman, Martin Naley
-
Publication number: 20090035809Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.Type: ApplicationFiled: May 29, 2008Publication date: February 5, 2009Applicant: INVITROGEN CORPORATIONInventors: Wai-Yee Leung, Ching-Ying Cheung, Stephen Yue
-
Publication number: 20090035857Abstract: Embodiments are directed to devices and methods for processing, cultivating or otherwise manipulating cell cultures which may be disposed on a flat or substantially flat surface such as cell culture substrate material. Devices and methods are disclosed for dividing a cell culture layer into divided portions, including isolated divided portions, that may then be transferred from the cell culture to a new location. For some embodiments, the divided portions may be transferred to a new cell culture support substrate in order to continue to grow and cultivate the cell line.Type: ApplicationFiled: July 25, 2008Publication date: February 5, 2009Applicant: INVITROGEN CORPORATIONInventors: Jonathan Chesnut, Robert Dees, Soojung Shin
-
Publication number: 20090029389Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.Type: ApplicationFiled: July 25, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventors: Kyle Richard Gee, Vladimir Martin
-
Publication number: 20090026079Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: July 11, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventors: Ilana MARGALIT, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel, Timothy Updyke
-
Publication number: 20090028838Abstract: The present invention provides a serum-free supplement which supports the growth of hematopoietic cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and for differentiating hematopoietic cells.Type: ApplicationFiled: August 15, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventors: John P. Daley, Barbara M. Dadey, William C. Biddle, Michelle G. Wysocki
-
Publication number: 20090029436Abstract: The present invention provides substantially pure reverse transcriptases, which are preferably substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in synthesizing, amplifying or sequencing nucleic acid molecules, including through the use of the polymerase chain reaction, particularly RT-PCR.Type: ApplicationFiled: October 1, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventor: A. John Hughes, JR.
-
Publication number: 20090023216Abstract: Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.Type: ApplicationFiled: July 11, 2007Publication date: January 22, 2009Applicant: INVITROGEN CORPORATIONInventor: Tod Woolf
-
Patent number: 7479573Abstract: Disclosed are compounds capable of facilitating transport of biologically active agents or substances into cells having the general structure: wherein Q is selected from the group consisting of N, O and S; L is any bivalent organic radical capable of linking each Q, such as C, CH, (CH2)l, or {(CH2)i—Y—(CH2)j}k, wherein Y is selected from the group consisting of CH2, an ether, a polyether, an amide, a polyamide, an ester, a sulfide, a urea, a thiourea, a guanidyl, a carbamoyl, a carbonate, a phosphate, a sulfate, a sulfoxide, an imine, a carbonyl, and a secondary amino group and wherein Y is optionally substituted by —X1—L?—X2—Z or —Z; R1—R6, independently of one another, are selected from the group consisting of H, —(CH2)p-D-Z, an alkyl, an alkenyl, an aryl, and an alkyl or alkyl ether optionally substituted by one or more of an alcohol, an aminoalcohol, an amine, an amide, an ether, a polyether, a polyamide, an ester, a mercaptan, an alkylthio, a urea, a thiourea, a guanidyl, or a carbamoyl group, and wType: GrantFiled: January 21, 2005Date of Patent: January 20, 2009Assignee: Invitrogen CorporationInventors: Yongliang Chu, Malek Masoud, Gulilat Gebeyehu
-
Publication number: 20090017546Abstract: The present invention provides novel reactive fluorescent compounds that incorporate stable isotopic (deuterium, 13-carbon, 15-nitrogen, 18-oxygen) substitutions. The invention includes the use of these compounds, in combination with non-isotopically substituted analogs, for the purification, identification and relative quantification of proteins, peptides, saccharides, metabolites, and other biologically important compounds by combining liquid chromatography (LC) and mass spectrometry (MS). Fluorescent labeling of target compounds in this manner provides orders-of-magnitude sensitivity enhancement over traditional stable isotope labels, and also affords the possibility of simultaneous multiplexed analysis due to the multiwavelength nature of different fluorophores.Type: ApplicationFiled: July 21, 2008Publication date: January 15, 2009Applicant: INVITROGEN CORPORATIONInventors: Brian Agnew, Kyle Richard Gee
-
Publication number: 20090004641Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.Type: ApplicationFiled: October 11, 2007Publication date: January 1, 2009Applicant: INVITROGEN CORPORATIONInventors: Kyle Richard Gee, Courtenay Rae Hart, Richard Haugland, Wayne Forrest Patton, Scott Whitney
-
Publication number: 20090004757Abstract: Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.Type: ApplicationFiled: June 23, 2008Publication date: January 1, 2009Applicant: Invitrogen CorporationInventors: Juan Yguerabide, Evangelina E. Yguerabide, David E. Kohne, Jeffrey T. Jackson