Abstract: It is a first object of the present invention to provide a method for preparing a Tile vector, being a vector, which comprises a selectable marker and a coding polynucleotide, wherein said coding polynucleotide is immediately preceded and followed by a type IIs recognition sequence, wherein said preceding and following recognition sequences are recognized by a same type IIs restriction enzyme, but have an opposite orientation. More particularly, the position and orientation of said preceding and following type IIs recognition sequences provides for the cleavage of said Tile vector by a corresponding type IIs restriction enzyme resulting in the release of said coding polynucleotide sequence having at its respective ends overhang sequences with a known orientation and length, while lacking said preceding and following type IIs recognition sequences.

Abstract: A system and method for simulating an electronic circuit is disclosed. The method includes creating a finite set of circuit or device parameter points selected from within an n-dimensional parameter space. The method includes determining, for each circuit or device parameter point of the set, a corresponding response value of the performance metric and a corresponding probability of occurrence. The method includes determining, for a predetermined value of the performance metric, the total probability of occurrence.

Abstract: A method for producing a three-dimensional component by means of a laser melting process, in which the component is produced by consecutively solidifying individual layers made of building material by melting the building material, wherein said building material can be solidified by the action of radiation, wherein the melting area produced by a punctiform and/or linear energy input is detected by a sensor device and sensor values are derived therefrom in order to evaluate the component quality. The sensor values detected in order to evaluate the component quality are stored together with the coordinate values that locate the sensor values in the component and are displayed by means of a visualization unit in two- and/or multi-dimensional representation with respect to the detection location of the sensor values in the component.

Abstract: The present invention concerns substituted indoline derivatives, methods to prevent or treat dengue viral infections by using said compounds and also relates to said compounds for use as a medicine, more preferably for use as a medicine to treat or prevent dengue viral infections. The present invention furthermore relates to pharmaceutical compositions or combination preparations of the compounds, to the compositions or preparations for use as a medicine, more preferably for the prevention or treatment of dengue viral infections. The invention also relates to processes for preparation of the compounds.

Abstract: A solid nanocomposite electrolyte material comprising a mesoporous dielectric material comprising a plurality of interconnected pores and an electrolyte layer covering inner surfaces of the mesoporous dielectric material. The electrolyte layer comprises: a first layer comprising a first dipolar compound or a first ionic compound, the first dipolar or ionic compound comprising a first pole of a first polarity and a second pole of a second polarity opposite to the first polarity, wherein the first layer is adsorbed on the inner surfaces with the first pole facing the inner surfaces; and a second layer covering the first layer, the second layer comprising a second ionic compound or a salt comprising first ions of the first polarity and second ions of the second polarity, wherein the first ions of the ionic compound or salt are bound to the first layer.

Abstract: Methods for detecting target analytes utilizing an array of wells are advantageous for detection of low concentrations of target analytes. Use of an array of wells requires sealing of the wells. The methods provided herein utilize digital microfluidics to seal wells of an array with a fluid that is immiscible with the aqueous liquid present in the wells to prevent evaporation and contamination of the aqueous fluid during analysis of signals from the wells. The disclosed method include generating a biphasic droplet composed of the immiscible fluid and an aqueous fluid. The immiscible fluid present in the biphasic droplet is moved over the array of wells to seal the wells by electrically actuating the aqueous fluid present in the biphasic droplet which in turn pulls the immiscible fluid.

Abstract: The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases that upon transformation of a eukaryotic microbial host cell, such as yeast, to confer to the host cell the ability of isomerising xylose to xylulose. The nucleic acid sequences encode xylose isomerases that originate from bacteria such as Eubacterium sp., Clostridium cellulosi and others. The invention further relates to fermentation processes wherein the transformed host cells ferment a xylose-containing medium to produce ethanol or other fermentation products.

Abstract: The present invention relates to a method for three dimensional printing of a porous object enabling the capillary transport of hydrophilic fluids, for use as liquid handling device, for example as a point of care diagnostic device. The invention also provides the porous object obtainable or obtained by such methods, and its use in liquid handling or as a point of care diagnostic device.

Abstract: A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term

Abstract: An analog-to-digital converter, ADC, circuitry, comprises: an integrator connected to a capacitor, the integrator being configured to switch between integrating an analog input signal for ramping an integrator output and integrating a reference input signal for returning integrator output towards a threshold; a comparator for comparing integrator output to the threshold; and a timer for determining a time duration during which the reference input signal is integrated, the time duration providing a digital representation of an analog input signal value; the ADC circuitry further comprising a feedforward noise shaping loop configured to store a quantization error signal based on digitizing a first sample, the comparator being configured to receive a feedforward noise shaping signal for changing the threshold for digitizing a later sample of the analog input signal following the first sample.

Abstract: The invention relates to polynucleotides comprising the sequence of a flavivirus preceded by a sequence encoding an N terminal part of a flavivirus Capsid protein, an immunogenic protein, or a part thereof comprising a an immunogenic peptide, and a 2A cleaving peptide, and to the virus encoded by such sequences. The invention further relates to the use of such polynucleotides and viruses as vaccines.

Abstract: Provided herein are methods for the production of a vector with a size of at least 16 kb from bacterial cells comprising the consecutive steps of a) obtaining bacterial cells comprising a vector with a size of at least 16 kb, comprising an inducible origin of replication, b) inoculating culture medium with the bacterial cells comprising the vector, c) culturing the bacterial cells in the culture medium, d) adding one or more inducers of said inducible origin of replication to the culture medium when the bacterial culture has reached an optical density at 600 nm (OD600) of at least 20, e) further culturing the bacterial cells in the culture medium, f) optionally separating the bacterial cells from the culture medium, and g) recovering the plasmid from the bacterial cells. Also provided herein are vectors with a size of at least 16 kb comprising an inducible origin of replication for use in such methods.

Abstract: A device for analysis of cells comprises: an integrated circuit arrangement on a substrate; a dielectric layer formed above the integrated circuit arrangement; a microelectrode array layer formed above the dielectric layer, said microelectrode array layer comprising a plurality of individual electrodes, wherein each electrode is connected to the integrated circuit arrangement through a via in the dielectric layer; and wherein a plurality of longitudinal trenches in the dielectric layer and the microelectrode array layer are for stimulating cell growth on a surface of the device

Abstract: A computer-implemented method of generating a visual representation of items comprising selecting a pair of items m and n comprising parameters representing a property, selecting a pair of parameters p and q, being amp the parameter p of item m, bmq the parameter q of item m, anp the parameter p of item n, and bnq the parameter q of item n, calculating a pair of weights wp and wq based on amp, bmq, anp and bnq, and based on amp, bmq, anp and bnq, storing, the pair of weights, determining a first vertex item comprising the greatest value for the parameter p, a second vertex item comprising the greatest value for the parameter q, and a third vertex comprising the greatest value for a parameter r, generating a plurality of points based on stored and determined values, and displaying a geometric shape comprising the plurality of points.

Abstract: A circuit is provided. The circuit includes a sampling circuit connectable to a multibit memory array and that samples a voltage across a sampling capacitor, a capacitance network including a plurality of capacitors and switching elements such that the capacitance network has a capacitance that depends on the configuration of the switching elements, and a buffering circuit configured to charge the capacitance of the capacitance network based on the voltage across the sampling capacitor. The circuit is configured to operate the capacitance network in a first state and a second state, wherein the capacitance in the states depends on an input value to the circuit. The circuit is also configured to charge the capacitance network in the first state and to allow the charge to redistribute within the capacitance network when it changes from the first to the second state. A system and method including such circuits are also provided.

Abstract: A configurable hardware device comprises a configuration memory of a known total size, and a configurable fabric arranged for being configured according to information from the configuration memory and segmented in a static partition and at least one dynamic partition. A static partition is arranged for receiving a bit stream and a cryptographic nonce and comprises a read/write mechanism for interacting with the configuration memory. The received bit stream is stored in the configuration memory and used to configure an intended application in the dynamic partition. The static partition is arranged for computing, based on the cryptographic nonce, a checksum of the entire configuration memory and for outputting the checksum.

Abstract: The present invention relates to a compound of formula (I) or a stereoisomer, or tautomer, (I) wherein A1, A2, R1 and R2, have the same meaning as that defined in the claims and the description. The present invention also relates to compositions, in particular pharmaceuticals, comprising such compounds, and to uses of such compounds and compositions for the prevention and/or treatment of an infection caused by pestivirus in an animal.

Abstract: A computer-implemented method of generating a visual representation of items comprising selecting a pair of items m and n comprising parameters representing a property, selecting a pair of parameters p and q, being amp the parameter p of item m, bmq the parameter q of item m, anp the parameter p of item n, and bnq the parameter q of item n, calculating a pair of weights wp and wq based on amp, bmq, anp and bnq, and based on amp, bmq, anp and bnq, storing, the pair of weights, determining a first vertex item comprising the greatest value for the parameter p, a second vertex item comprising the greatest value for the parameter q, and a third vertex comprising the greatest value for a parameter r, generating a plurality of points based on stored and determined values, and displaying a geometric shape comprising the plurality of points

Abstract: The present invention relates to the synthesis of 6-substituted 7-deazapurines and their corresponding nucleosides by coupling aryl or alkyl Grignard reagents with halogenated purine nucleosides in the presence of iron or an iron/copper mixture such as Fe(acac)3/CuI. The present invention also relates to pharmaceutical compositions comprising said compounds and the use of said pharmaceutical compositions to treat or prevent viral infections.

Abstract: Porous solid materials are provided. The porous solid materials include a plurality of interconnected wires forming an ordered network. The porous solid materials may have a predetermined volumetric surface area ranging between 2 m2/cm3 and 90 m2/cm3, a predetermined porosity ranging between 3% and 90% and an electrical conductivity higher than 100 S/cm. The porous solid materials may have a predetermined volumetric surface area ranging between 3 m2/cm3 and 72 m2/cm3, a predetermined porosity ranging between 80% and 95% and an electrical conductivity higher than 100 S/cm. The porous solid materials (100) may have a predetermined volumetric surface area ranging between 3 m2/cm3 and 85 m2/cm3, a predetermined porosity ranging between 65% and 90% and an electrical conductivity higher than 2000 S/cm. Methods for the fabrication of such porous solid materials and devices including such porous solid material are also disclosed.