Abstract: [Problem to be Solved] To provide a liquid laundry detergent composition for clothing which can remove bacteria from the clothing effectively during washing and also impart an excellent antibacterial effect to the clothing after washing.
Abstract: A rupture disk includes a body including an internal wall extending from a first end of the body to a second end of the body. The internal wall defines an opening extending through the body. In some embodiments, the body further includes an internal flange formed on the internal wall, and the internal flange has a stepped surface. A rupture membrane is positioned within the body, and secured, for example, by cold welding. The rupture membrane may be secured to the stepped surface. The rupture membrane is positioned within the body such that the rupture membrane does not extend beyond the first end of the body. A process system including the rupture disk is also provided. A method of maintaining a process system that includes the rupture disk is also provided.
Abstract: The invention relates to an isolated nucleic acid sequence comprising a promoter, which is a native sequence of Pichia pastoris comprising the nucleic acid sequence of pCS1 of SEQ ID 1, or a functionally active variant thereof which is a size variant, a mutant or hybrid of SEQ ID 1, or a combination thereof, expression constructs and recombinant host cells comprising the promoter, and a method of producing a protein of interest under the control of the promoter. It further relates to a method to identify a constitutive promoter from eukaryotic cells, and an isolated nucleic acid sequence comprising a promoter which when operatively linked to a nucleotide sequence encoding a protein of interest directs the expression thereof in a host cell at an expression level that is higher than under control of the native pGAP promoter at high and low growth rates.
Type:
Grant
Filed:
December 18, 2013
Date of Patent:
October 1, 2019
Assignee:
Lonza Ltd
Inventors:
Diethard Mattanovich, Brigitte Gasser, Roland Prielhofer
Abstract: Disclosed herein are methods and compositions useful for detecting and/or quantifying host cell proteins during the production of a product, e.g., a recombinant protein, e.g., an antibody.
Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate or at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.
Type:
Grant
Filed:
October 26, 2016
Date of Patent:
May 28, 2019
Assignee:
LONZA LTD.
Inventors:
Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
Abstract: The present invention relates to method for connecting a protein and a drug to a protein drug conjugate, wherein the drug is linked to the protein through a specific branched linker, said branched linker comprises a peptide chain and is derived from o-hydroxy p-amino benzylic alcohol, wherein the peptide chain is connected to the phenyl ring via the p-amino group, the drug is connected to the phenyl ring via the benzylic alcohol moiety, and the protein is connected to the phenyl ring via the o-hydroxy group; further to a process for the preparation of said protein-drug-conjugates via various intermediates, to the pharmaceutical use of such protein drug conjugates, such as methods of controlling the growth of undesirable cells, to pharmaceutical compositions comprising such protein drug conjugates, and to intermediates of the preparation of the protein drug conjugates.
Type:
Grant
Filed:
February 23, 2012
Date of Patent:
May 21, 2019
Assignees:
LONZA LTD, Lonza Guangzhou Nansha Ltd.
Inventors:
Laurent Ducry, Bernhard Stump, Heilam Wong, Jin She, Gayle Allway
Abstract: The disclosure is directed to a system, apparatus, and method for continuously monitoring data output from a wireless sensor on a mesh network at a frequency higher than mesh formation/propagation. For example, the system may include a wireless gateway, an amplifier coupled to the wireless gateway, a first antenna coupled to the amplifier, a second antenna coupled to the amplifier, and a wireless sensor. The wireless sensor may be a wireless temperature sensor configured to monitor the temperature of a cryopreserved material product. The wireless gateway forms the mesh network. The first antenna may be located on a first level of a building, and the second antenna may be located on a second level of the building, where the second level may be at an elevation higher than the first level.
Abstract: The present invention relates to method for connecting a protein and a drug to a protein drug conjugate, wherein the drug is linked to the protein through a specific branched linker, said branched linker comprises a peptide chain and is derived from o-hydroxy p-amino benzylic alcohol, wherein the peptide chain is connected to the phenyl ring via the p-amino group, the drug is connected to the phenyl ring via the benzylic alcohol moiety, and the protein is connected to the phenyl ring via the o-hydroxy group; further to a process for the preparation of said protein-drug-conjugates via various intermediates, to the pharmaceutical use of such protein drug conjugates, such as methods of controlling the growth of undesirable cells, to pharmaceutical compositions comprising such protein drug conjugates, and to intermediates of the preparation of the protein drug conjugates.
Type:
Grant
Filed:
March 23, 2018
Date of Patent:
February 26, 2019
Assignees:
LONZA LTD, Lonza Guangzhou Nansha Ltd.
Inventors:
Laurent Ducry, Bernhard Stump, Heilam Wong, Jin She, Gayle Allway
Abstract: An isolated nucleic acid encoding a leader, which has a specific sequence, an isolated leader peptide encoded by such nucleic acid, an expression cassette comprising such nucleic acid encoding a leader operably linked to a nucleic acid sequence encoding a POI, a recombinant yeast host cell or a vector comprising such expression cassette, a method of producing a POI in such yeast host cell, and further the use of the specific nucleic acid for the secretion of a POI from a host cell and/or to increase the secretion of a POI from a host cell.
Abstract: The invention relates to the use of L-carnitine, a salt of L-carnitine, a derivative of L-carnitine and/or salt of a derivative of L-carnitine, as well as respective methods and compositions, for reducing or preventing fatigue and/or for improving cognitive function in an animal. The animal is preferably a healthy individual and the use is preferably a non-therapeutic use.
Type:
Grant
Filed:
April 4, 2012
Date of Patent:
November 20, 2018
Assignee:
Lonza Ltd.
Inventors:
Adriana Williams, Frauke Warrikoff, Ulla Freitas, Richard Sasse, Kevin Owen
Abstract: The invention discloses a method for the continuous preparation of n-butyl nitrite with a low content of n-butanol comprising the reaction of n-butanol, an acid and NaNO2 in a continuous way, in which the n-butanol, an acid and NaNO2 are mixed in a mixing device which provides for a pressure drop of at least 1 bar; the acid is selected from the group consisting of HCI, H2SO4, formic acid, methanesulfonic acid, and mixtures thereof; and the amount of HCI is at least 1.02 molar equivalent based on the molar amount of n-butanol.
Type:
Grant
Filed:
September 14, 2016
Date of Patent:
November 13, 2018
Assignee:
LONZA LTD
Inventors:
Christoph Taeschler, Ulrich Mayerhoeffer, Christophe Girard, Michael Bittel, Rolf Kalbermatten, Claudio Arnold, Stefan Garms, Christian Schnider, Stefan Tille, Manuel Favre, Guy Raboud, Kilian Schnydrig, Martin Venetz, Thomas Eggel
Abstract: The invention discloses a method for the preparation of thiophene-2-carbonyl chlorides starting from thiophenes with oxalyl chloride at elevated temperature with short reaction time.
Type:
Grant
Filed:
November 2, 2016
Date of Patent:
October 30, 2018
Assignee:
LONZA LTD
Inventors:
Daniel Zollinger, Florencio Zaragoza Doerwald
Abstract: A method for preventing plugging of a continuous-reaction channel-system caused by a by-product of a continuous-reaction being carried out in said channel-system comprises the step of generating at least one ultrasonic wave travelling through said channel-system by coupling in a flow direction of at least one process fluid of a plurality of process fluids said at least one ultrasonic wave into said at least one process fluid.
Type:
Grant
Filed:
August 26, 2010
Date of Patent:
August 28, 2018
Assignee:
LONZA LTD.
Inventors:
Dominique Roberge, Fabio Rainone, Wilhelm Quittmann, Michael Gottsponer, Markus Eyholzer
Abstract: The invention discloses a method for the preparation of 1-methyl-3-(trifluoromethyl)-1H-pyrazol-5-ol with high selectivity with respect to the content of the isomer 1-methyl-5-(trifluoromethyl)-1H-pyrazol-3-ol.
Type:
Application
Filed:
November 14, 2016
Publication date:
August 16, 2018
Applicant:
LONZA LTD
Inventors:
Christoph Taeschler, Christophe Girard, Sandro Tonazzi, Daniel Polenske, Stefan Garms, Christoph Escher, Andreas Lorenz, Sonja Biner, Mareile Von Der Gruen, Herbert Kuehberger, Jonas Zurbriggen, Dieter In-Albon, Beat Lutz
Abstract: The invention relates to a topical composition containing a bilirubin-producing plant extract. In particular, the bilirubin-producing plant extract is obtained from the genus Stelitzia. When topically applied to skin, the composition is effective in accelerating the degradation of heme by-products such as bilirubin present in the skin.
Abstract: The present disclosure features methods and compositions for increasing the amount of products of cellular metabolism, e.g., proteins, by lowering the temperature of cells expressing the product at one or more steps while culturing the cells, expressing the product, and/or recovering the product.
Abstract: The invention discloses a method for preparation of fluoro, chloro and fluorochloro alkylated compounds by homogeneous Pd catalyzed fluoro, chloro and fluorochloro alkylation with fluoro, chloro and fluorochloroalkyl halides in the presence of di(1-adamantyl)-n-butylphosphine and in the presence of 2,2,6,6-tetramethylpiperidine 1-oxyl.
Type:
Application
Filed:
November 5, 2015
Publication date:
November 9, 2017
Applicant:
Lonza Ltd
Inventors:
Christoph Taeschler, Florencio Zaragoza Doerwald, Stefan Ellinger, Matthias Beller, Helfried Neumann, Lin He, Kishore Natte
Abstract: The invention discloses a method for preparations of fluoro, chloro and fluorochloro alkylated compounds by homogeneous Pd catalyzed fluoro, chloro and fluorochloro alkylation with fluoro, chloro and fluorochloroalkyl halides in the presence of di(1-adamantyl)-adamantyl-n-butylphosphine and in the presence of 2,2,6,6-tetramethylpiperdine 1-oxyl.
Type:
Grant
Filed:
November 5, 2015
Date of Patent:
November 7, 2017
Assignee:
Lonza Ltd.
Inventors:
Christoph Taeschler, Florencio Zaragoza Doerwald, Stefan Ellinger, Matthias Beller, Helfried Neumann, Lin He, Kishore Natte
Abstract: The invention discloses a method for preparation of alkylated or fluoro, chloro and fluorochloro alkylated compounds by a heterogeneous Pt/C-catalyzed alkylation or fluoro, chloro and fluorochloro alkylation with alkyl halides or with fluoro, chloro and fluorochloro alkyl halides in the presence of Cs2C03 or CsHC03.
Type:
Grant
Filed:
June 4, 2015
Date of Patent:
September 26, 2017
Assignee:
LONZA LTD
Inventors:
Christoph Taeschler, Matthias Beller, Helfried Neumann, Lin He, Kishore Natte, Stefan Ellinger, Florencio Zaragoza Doerwald