Patents Assigned to Perlegen Sciences, Inc.
  • Methods for amplification of nucleic acids
    Publication number: 20030073093
    Abstract: The presently claimed invention provides methods for amplifying a DNA target sequence. One embodiment of the present invention provides robust methods for amplification of target sequences. In a first aspect of the invention, a method for designing primer pairs for the amplification reaction is provided. In a further aspect of the invention, reagents and cycling parameters for the amplification reaction are provided.
    Type: Application
    Filed: January 9, 2002
    Publication date: April 17, 2003
    Applicant: Perlegen Sciences, Inc.
    Inventors: Curtis Kautzer, Nila Patil, Coleen Hacker, David P. McDonough
  • Algorithms for selection of primer pairs
    Publication number: 20030068625
    Abstract: The presently claimed invention provides methods for amplifying a DNA target sequence. One embodiment of the present invention provides robust methods for amplification of target sequences. In a first aspect of the invention, a method for designing primer pairs for the amplification reaction is provided. In a further aspect of the invention, reagents and cycling parameters for the amplification reaction are provided.
    Type: Application
    Filed: January 9, 2002
    Publication date: April 10, 2003
    Applicant: Perlegen Sciences, Inc.
    Inventors: John B. Sheehan, Daryl J. Thomas, Wade A. Barrett
  • Methods for identifying the evolutionarily conserved sequences
    Publication number: 20030017474
    Abstract: The present invention provides methods for determining sequence similarity (conserved sequences) between nucleic acids from a first organism and nucleic acids from a second, different organism without having to know a priori the nucleic acid sequence from the second, different organism. The first nucleic acid can be from any organism where the sequence of the nucleic acid is known and the second nucleic acid can be from any organism. The method involves determining which bases from the second nucleic acid are identical to the first nucleic acid, and allows one to determine the sequence of portions of the second nucleic acid. The invention is useful for identifying putative functional regions or putative organism-sequences in a genome.
    Type: Application
    Filed: October 5, 2001
    Publication date: January 23, 2003
    Applicant: Perlegen Sciences, Inc., a Delaware corporation
    Inventors: Kelly A. Frazer, Nila Patil, John Sheehan
  • Methods for reducing complexity of nucleic acid samples
    Publication number: 20020164634
    Abstract: The invention provides several methods for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids on a nucleic acid probe array. The methods result in a subset of the initial population enriched for a desired property, or lacking nucleic acids having an undesired property. The resulting nucleic acids in the subset are then applied to the array for various types of analysis. The methods are particularly useful for analyzing populations having a high degree of complexity, for example, chromosomal-derived DNA, or whole genomic DNA, or mRNA population. In addition, such methods allow for analysis of pooled samples.
    Type: Application
    Filed: April 24, 2002
    Publication date: November 7, 2002
    Applicant: Perlegen Sciences, Inc.
    Inventors: Nila Patil, David R. Cox, Charit Pethiyagoda, Kelly A. Frazer