Abstract: Nucleic acid catalysts, method of screening/selection for nucleic acid catalysts, synthesis of ribozyme libraries and discovery of gene sequences involved in a biological process are described.
Abstract: An enzymatic nucleic acid molecule which cleaves an immunodeficiency virus RNA in a gene required for viral replication, e.g., the nef or tat gene regions.
Type:
Grant
Filed:
February 12, 1999
Date of Patent:
December 12, 2000
Assignee:
Ribozyme Pharmaceuticals, Inc.
Inventors:
Kenneth G. Draper, Bharat Chowrira, James McSwiggen, Dan T. Stinchcomb, James D. Thompson
Abstract: A transcribed non-naturally occuring RNA molecule comprising a desired RNA molecule, wherein the 3' region of the RNA is able to base-pair with at least 8 bases at the 5' terminus of the same RNA molecule.
Abstract: Nucleozymes containing ribonucleotides and deoxyribonucleotides or nucleic acid analogues are described herein. The nucleozymes have catalytic activity and are significantly more resistant to degradation than their all-RNA ribozyme counterparts. Also described are methods for preparing the nucleozymes along with methods of using nucleozymes, e.g., as therapeutic agents.
Type:
Grant
Filed:
June 2, 1995
Date of Patent:
October 31, 2000
Assignee:
Ribozyme Pharmaceuticals, Inc.
Inventors:
Nassim Usman, Alexander Rich, Robert J. Cedergren, Jean-Pierre Perreault, Jing-Hua Yang
Abstract: Novel nucleoside triphosphates, methods of synthesis and process of incorporating these nucleoside triphosphates into oligonucleotides are disclosed.
Type:
Grant
Filed:
November 4, 1998
Date of Patent:
October 3, 2000
Assignee:
Ribozyme Pharmaceuticals, Inc.
Inventors:
Leonid Beigelman, Alex Burgin, Amber Beaudry, Alexander Karpeisky, Jasenka Matulic-Adamic, David Sweedler
Abstract: Oligonucleotide comprising a nucleotide base having the formula: ##STR1## wherein B is a nucleotide base or hydrogen; R.sub.1, R.sub.2 and R.sub.3 independently is selected from the group consisting of hydrogen, an alkyl group containing between 2 and 10 carbon atoms inclusive, an amine, an amino acid, and a peptide containing between 2 and 5 amino acids inclusive; and the zigzag lines are independently hydrogen or a bond.
Type:
Grant
Filed:
February 10, 1998
Date of Patent:
July 25, 2000
Assignee:
Ribozyme Pharmaceuticals, Inc.
Inventors:
Lech Dudycz, Jasenka Matulic-Adamic, Leonid Beigelman
Abstract: Hairpin ribozyme lacking a substrate moiety, comprising atleast six bases in helix 2 and able to base-pair with a separate substrate RNA, wherein the said ribozyme comprises one or more bases 3' of helix 3 able to base-pair with the said substrate RNA to form a helix 5 and wherein the said ribozyme can cleave and/or ligate said separate RNA(s) in trans.
Abstract: An enzymatic nucleic acid molecule comprising a 5'- and/or a 3'-cap structure, wherein said structure is not a 5'-5'-linked inverted nucleotide or a 3'-3'-linked inverted nucleotide.
Type:
Grant
Filed:
April 16, 1996
Date of Patent:
December 7, 1999
Assignee:
Ribozyme Pharmaceuticals, Inc.
Inventors:
Jasenka Matulic-Adamic, Leonid Beigelman, Alexander Karpeisky, Thale Jarvis, Nassim Usman, Anthony DiRenzo, Francine Wincott