Abstract: Provided are an engineering probiotic for degrading uric acid, and a construction method therefor and the use thereof, which belongs to the field of biotechnology. The probiotic Escherichia coli Nissle 1917 is used as an original starting strain, and an exogenous gene is introduced into a genome thereof by means of genetic engineering technology for modification, such that uric acid can be efficiently and rapidly degraded; and it is proved by tests that the rapid degradation of uric acid can be achieved in the intestinal tract and blood of mice. Compared with current lactic acid bacteria for degrading uric acid in the market, the constructed engineering probiotic has a stronger degradation ability and a better treatment effect, and therefore has a good practical application value.

Abstract: Provided are a highly specific Taq DNA polymerase variant and the use thereof in genome editing and gene mutation detection. All polar amino acids, directly interacting with a primer/template complex, on a Taq enzyme are selected to be mutated one by one to obtain 40 Taq variants, and extensive random mutagenesis is performed on the basis of the sequences of the variants and the sequences of wild type Taq enzymes to create a Taq mutant library. Then, a series of Taq mutants with a high specificity are screened on a qPCR screening system by means of taking a genome editing indels plasmid as a template, wherein the Taq mutants exhibit great advantages in CRISPR/Cas9 editing efficiency evaluation and single-cell cloning genotyping.

Abstract: A method for synthesizing and application embedded alkaline earth metal oxide solid alkali includes: firstly, synthesizing an alkaline earth metal organic skeleton with single or multiple alkaline earth metals (Mg, Ca and Sr) as central metal elements; and then controlling the heating process to carry out high-temperature pyrolysis in a non-oxidizing atmosphere, so that the alkaline earth metal oxide are embedded in the nano carbon sheet to obtain a solid alkali catalyst. Finally, the catalyst is used to catalyze the transesterification of palm oil and methanol to produce biodiesel. The active site of the solid alkali obtained by the method is anchored on the nano-like carbon sheet, so that the active site is directly exposed on the surface of the catalyst, the catalytic activity is improved, the loss of the active site is inhibited, and the stability of the solid alkali catalyst is enhanced.

Abstract: The present disclosure discloses method for constructing a locomotive collaborative transportation scheduling system based on cyber-physical fusion, which provides a solution for multi-processor coordinated control of locomotive scheduling. The method includes the following steps: step 1, planning the actual on-site location of the device; step 2: constructing a distributed cyber-physical system collaborative architecture; step 3: describing the information transmission relationship between physical devices in a matrix manner to construct an information collaboration computing model; step 4: analyzing and selecting the matching controller type according to the device function implementation program; step 5: writing the controller function scheduling program to complete the construction of the locomotive collaborative transportation scheduling cyber-physical system, and realizing the locomotive automatic scheduling through this system.

Abstract: An adjustment method of moisture content and dense state for a hydrogel improved subgrade based on weather-resistance during an in-service period, including: step 1: carrying out surface cleaning and compaction of ground; step 2: preparing hydrogel improved subgrade raw material; step 3: paving the prepared material on the surface to form first-layer improved subgrade; and step 4: paving plain soil subgrade onto the first-layer. The method combines the water absorption and release function of the modified resin and the characteristic of the gel state thereof, to pave the improved subgrade in layers, which can absorb water and slightly expand when the water content in the subgrade is increased to a certain threshold value, and a strength and compactness protective layer can also be formed at the connection sections of the ground and the subgrade, and the subgrade and the pavement, to prevent the pot-cover effect from occurring.

Abstract: Disclosed is an in-vivo micro-robot for nerve stretching, comprising a channel, and a movable part and a fixed part located at the channel. The movable part is disposed to be movable along the channel, the movable part sleeves the fixed part and is used for driving the fixed part to move along the channel, and the fixed part is connected to a nerve to be stretched. The in-vivo micro-robot is embedded into a patient's body through surgery, so that the extension of the nerve is accelerated and is regular and quantitative, thereby effectively solving the problems of small probability of nerve self-repair and long recovery period in traditional nerve bridging surgery.

Abstract: A method and system for generating a high-order pseudo-random electromagnetic exploration signal. The method includes: constructing two or more basic unit signals according to an exploration requirement, wherein the basic unit signals are stairstep signals obtained by superposing a plurality of in-phase periodic square wave signals, and a frequency ratio between adjacent ones of the plurality of periodic square wave signals is 2; and superposing the two or more basic unit signals to obtain superposed stairstep signals, and correcting amplitudes to be consistent with amplitudes of the periodic square wave signals, to obtain high-order 2n sequence pseudo-random signals. The 2n sequence stairstep signals of different orders can be constructed within a limited frequency interval.

Abstract: It discloses a sustained release grouting material, a preparation method and an application thereof; the sustained release grouting material comprises a water swelling core and a water-soluble sustained release coating, the water swelling core is composed of bentonite, SAP and xanthan gum, which is granulated by disc granulator relying on the cohesiveness of xanthan gum; the water-soluble sustained-release coating is prepared by mixing water-soluble starch, polyvinyl alcohol, poloxamer, lubricant and plasticizer with deionized water to obtain a film-forming solution, and the film-forming solution is coated on a coating machine; the invention uses a coating machine to coat a layer of slow-release coating on the surface of the water-absorbing expansion core, and adjusts the coating thickness by controlling the mass ratio of the coating film-forming liquid to the water-absorbing expansion core particles, so as to realize the controllable water absorption onset time of the expansion core.

Abstract: The present invention relates to an ethanol foam sclerosing agent for vascular anomalies and a preparation method thereof. The ethanol foam sclerosing agent includes absolute ethanol, water, Tween 80 and a stabilizer. The ethanol foam sclerosing agent further includes hyaluronic acid. The mass composition of the ethanol foam sclerosing agent includes 32-42% of absolute ethanol, 0.5-2% of Tween 80, 0-25% (excluding 0) of egg yolk lecithin, 0-2% (excluding 0) of hyaluronic acid, and the balance of water. The stabilizer is hyaluronic acid or glycerin. Foam is prepared by the Tessari method. While the original therapeutic effect of ethanol is not changed, the side effect of the ethanol is significantly reduced.

Abstract: A BTA drilling quick-stop device (QSD) and a method, wherein the BTA drilling QSD includes a base. The base includes a hollow cavity. A side portion of the base has an opening communicating with the hollow cavity. The opening is closed by a through cover. A horizontally movable fixture is disposed in the hollow cavity. A stopper is disposed at a top portion of the base. A bottom portion of the stopper is disposed lower than a top portion of the fixture to stop the fixture from moving during processing. An elastic element is disposed between the through cover and a side portion of the fixture to push the fixture to move after processing.

Abstract: Disclosed are an automatic stable posture seabed base and a releasing method, belonging to the technical field of ocean observation. The automatic stable posture seabed base includes an accommodating body with an inner cavity for accommodating the first observation device and a seawater, and the accommodating body has first through holes for the seawater to enter and outflow the inner cavity; plugging members blocked at the first through holes to close or semi-close the first through holes; and a buoyancy body installed at a transducer of the first observation device, and a buoyancy of the buoyancy body is larger than a mass of the first observation device, so that the first observation device may float in the cover body after the cover body is filled with water, and a posture of the transducer in the cover body always keeps vertically to the sea surface.

Abstract: The present invention relates to a mechanical tunneling apparatus and process for a tunnel in a quicksand stratum. The mechanical tunneling apparatus comprises isolating apparatuses arranged at an upper end and a lower end of a supporting apparatus; an excavation apparatus arranged at a front end of the supporting apparatus; a spraying apparatus arranged on the isolating apparatuses and the excavation apparatus; a muck discharging apparatus arranged at a lower part of the supporting apparatus; and a propulsion apparatus arranged at a rear end of the supporting apparatus.

Abstract: A discrete element method contact model building method includes: selecting a filling body in a disaster-causing structure to obtain a change rule of cumulative loss of the filling body; performing test simulation, and determining a relation function of each group of corresponding mesoscopic mechanical parameters in each time period and mesoscopic parameters of a DEM contact model representing a change rule of macroscopical parameters of the filling body; embedding each mesoscopic parameter relation function into an existing particle contact model, performing test simulation, and updating a fracture failure criterion of the contact model according to a corresponding relation of macro-mesoscopic strength during model failure; and based on a seepage failure indoor test, building a seepage failure discrete element calculation model, and simulating the seepage failure process of a rock and soil mass by using the obtained particle contact model and the fracture criterion of the particle contact model.

Abstract: A transmit beamforming method for integrated multi-antenna systems with joint near-field sensing and far-field communication, which includes: (1) establishing a communication signal transmission model and a communication channel model, and analyzing an upper limit of communication spectrum efficiency; (2) acquiring an optimal communication beam matrix based on the communication channel model; (3) establishing a near-field sensing signal model; (4) generating an optimal radar beam matrix based on an antenna array response vector of a radar detection target; (5) introducing a compromise factor to construct an initial optimization problem of an integrated beamforming matrix in power constraint based on the established near-field sensing signal model; and (6) transforming and solving the constructed initial optimization problem to obtain the integrated beamforming matrix.

Abstract: A method for preparing a shell-bionic ceramic tool and a shell-bionic ceramic tool, wherein the shell-bionic ceramic tool includes alternating stacks of ceramic powders with different components, pressing a ceramic green body using a cold briquetting method, carrying out pre-pressing once using a graphite indenter on a working surface thereof after each layer of the ceramic powder being loaded, and pressing a last layer using a graphite rod, and then pressing a whole ceramic green body with a certain pressure to promote a bonding of the layers of ceramic powder, which in turn gives a complex shape to an interface between the layers, increases a bonding area between the layers, and plays the role of hindering crack expansion, extending the crack expansion path, and improving the bonding strength of the interface; after then, hot-pressed sintering is used to densify the ceramic green body to obtain the shell-bionic ceramic tool.

Abstract: The present disclosure relates to a NiTi alloy surface cutting process and a roughness adjustment method. Aiming at defects of difficult machining and unsatisfactory machining performance of Ni—Ti alloys, the present disclosure provides a surface cutting process of a difficult-to-machine nickel-titanium alloy, in which firstly, the nickel-titanium alloy is transformed from a two-phase state to a single-phase state using its transformation characteristic, and electrochemical nickel removal treatment is carried out on a surface of the alloy in the single-phase state so as to obtain a porous surface layer, and then a surface material with the porous surface layer is cut. The method provided by the present disclosure can effectively reduce machining difficulty of alloy materials, and can also obtain alloy workpieces with adjustable roughness, which is of great significance for NiTi alloy machining.

Abstract: Disclosed are a shield tunnel segment structure and a construction method thereof. The shield tunnel segment structure includes segment blocks sequentially spliced in a circumferential direction. Each segment block forms a closed annular segment structure, and outer diameters of adjacent annular segment structures gradually increase in an axial direction. At least two adjacent segment blocks of the same annular segment structure form an annular inner groove, and at least one segment block of the adjacent annular segment structures is provided with an inner bump which matches the annular inner groove. At least two adjacent segment blocks of the same annular segment structure form an annular outer groove, and at least one segment block of the adjacent annular segment structures is provided with an outer bump which matches the annular outer groove. The annular outer grooves and the annular inner grooves are staggered in the circumferential direction.

Abstract: A three-dimensional electrical resistivity tomography method and system belonging to the field of geological geophysical prospecting, the method including the steps of prospecting a region containing a geological anomaly with at least two prospecting modes respectively to acquire two-dimensional resistivity data of a corresponding detection plane; unifying coordinate systems of resistivity data points acquired in all prospecting modes, and extracting data points with the same coordinates; carrying out data fusion on extracted resistivity data at the same position by utilizing a principal component analysis method; and carrying out three-dimensional coordinate conversion on resistivity data acquired after fusion to form a three-dimensional model.

Abstract: A multi-fault diagnosis method has the following steps: measuring cell voltages of a battery pack to be diagnosed; constructing a cell voltage sequence according to measured cell voltages of the battery pack to be diagnosed, and calculating a sample entropy value of the cell voltage sequence; setting a correction coefficient for representing voltage fluctuation information, and correcting the sample entropy value through the correction coefficient to obtain a corrected sample entropy value; and judging and outputting a fault type of the battery pack to be diagnosed according to a numerical value change of the corrected sample entropy value. Faults of cells can be accurately diagnosed without a model, sample entropy values under different faults can be distinguished by setting the correction coefficient, the intuitiveness and efficiency of fault detection are improved, and the fault type and time of the lithium-ion cells can be quickly, accurately and stably diagnosed and predicted.

Abstract: The present invention relates to an experimental apparatus for researching characteristics of subsea tunnel at bottom of seabed under wave, comprising main control system, wave making test system, rock and soil confining pressure test system and water level regulation test system, wherein a bottom of the rock and soil confining pressure test system is connected with the water level regulation test system; a bottom of the wave making test system is connected with the rock and soil confining pressure test system; and all test systems are electrically connected with the main control system. The experimental apparatus, when being small, may simulate the situation of the subsea tunnel under a nonlinear complex wave, may consider the common action of the seabed and the wave, and may regulate water levels and soil pressures, having perfect functions and effectively exploring the effect of various aspects on the subsea tunnel.