Patents Assigned to Takara Bio, Inc.
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Publication number: 20140363849Abstract: Provided are the following: a method, for improving reactivity of a nucleic acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.Type: ApplicationFiled: January 24, 2013Publication date: December 11, 2014Applicant: Takara Bio Inc.Inventors: Kiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
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Patent number: 8889844Abstract: A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.Type: GrantFiled: August 10, 2006Date of Patent: November 18, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Kazuya Matsumoto, Junichi Mineno, Ikunoshin Kato
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Publication number: 20140322793Abstract: Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A737-A738-A739-A740-A741-A742-A743-A744-A745-” between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: A737 is an amino acid residue having a non-polar aliphatic side chain; A738 is an amino acid residue having a non-polar aliphatic side chain; A739 is an amino acid residue having a positively charged side chain; A740 is an amino acid residue having a positively charged side chain; A741 is an amino acid residue having a non-polar aliphatic side chain; A742 is an amino acid residue having a non-polar aliphatic side chain; A743 is any given amino acid residue; A744 is an amino acid residue having a positively charged side chain; and A745 is an amino acid residue having a non-polar aliphatic side chain).Type: ApplicationFiled: July 12, 2012Publication date: October 30, 2014Applicants: TAKARA BIO INC., KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATIONInventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa, Takashi Uemori, Takehiro Sagara
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Patent number: 8852915Abstract: A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.Type: GrantFiled: October 12, 2012Date of Patent: October 7, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Yasushi Katayama, Hiromi Okuyama, Junichi Mineno, Kiyozo Asada, Ikunoshin Kato
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Patent number: 8841126Abstract: Disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which is simple and highly efficient. Specifically disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a culture vessel on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 4 hours or longer, thereby producing a culture vessel having the retrovirus vector bound thereto, and (b) adding a target cell to the culture vessel that has been produced in step (a) and incubating the culture vessel. The gene introduction method is simple and highly efficient, and is useful particularly in the fields of medicine, cell technology, gene technology, embryologic technology and the like.Type: GrantFiled: June 29, 2011Date of Patent: September 23, 2014Assignee: Takara Bio Inc.Inventors: Katsuyuki Dodo, Naoki Saito, Hideto Chono, Junichi Mineno
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Patent number: 8835177Abstract: A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.Type: GrantFiled: June 12, 2006Date of Patent: September 16, 2014Assignee: Takara Bio Inc.Inventors: Takahiro Marui, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
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Publication number: 20140242701Abstract: Provided are a chimeric antigen receptor comprising an extracellular domain capable of binding to an antigen, a transmembrane domain and at least one intracellular domain, the chimeric antigen receptor being characterized in that an intracellular domain of a glucocorticoid-induced tumor necrosis factor receptor (GITR) is contained as the intracellular domain; a nucleic acid encoding the chimeric antigen receptor; a cell expressing the chimeric antigen receptor; and a method for producing the cell.Type: ApplicationFiled: October 5, 2012Publication date: August 28, 2014Applicant: TAKARA BIO INC.Inventors: Hiroshi Shiku, Yuki Orito, Junichi Mineno, Sachiko Okamoto, Yasunori Amaishi
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Patent number: 8815597Abstract: The present invention provides a baglike container for centrifugation that is mounted in a centrifuge to thereby allow centrifugation of a dispersion liquid accommodated therein. The baglike container for centrifugation is less likely to tear or break as a result of centrifugation by disposing a container wall surface of the baglike container so as to apply centrifugal force perpendicular to the container wall surface.Type: GrantFiled: August 23, 2007Date of Patent: August 26, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Junichi Mineno, Kazutoh Takesako, Takao Yoshida, Takashi Morimura, Kenji Sakai, Shin-ichi Yamada, Noritsugu Yabe, Yuko Taguchi
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Publication number: 20140212972Abstract: The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof.Type: ApplicationFiled: April 10, 2014Publication date: July 31, 2014Applicant: TAKARA BIO INC.Inventors: Hiroaki SAGAWA, Mitsuko IDENO, lkunoshin KATO
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Patent number: 8765469Abstract: A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.Type: GrantFiled: August 10, 2006Date of Patent: July 1, 2014Assignee: Takara Bio Inc.Inventors: Takahiro Marui, Kinuko Nagamine, Nobuko Muraki, Akiko Kato, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
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Publication number: 20140170709Abstract: The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections.Type: ApplicationFiled: January 31, 2014Publication date: June 19, 2014Applicant: TAKARA BIO INC.Inventors: Hideto CHONO, Kazuya MATSUMOTO, Hajime MATSUMURA, Junichi MINENO, Ikunoshin KATO
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Patent number: 8728811Abstract: The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof.Type: GrantFiled: March 25, 2003Date of Patent: May 20, 2014Assignee: Takara Bio Inc.Inventors: Hiroaki Sagawa, Mitsuko Ideno, Ikunoshin Kato
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Publication number: 20140120124Abstract: Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.Type: ApplicationFiled: October 15, 2013Publication date: May 1, 2014Applicants: TAKARA BIO INC., MIE UNIVERSITYInventors: Hiroshi SHIKU, Hiroaki IKEDA, Jun MITSUI, Yuki TAKENAKA, Junichi MINENO, Ikunoshin KATO
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Patent number: 8586023Abstract: Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.Type: GrantFiled: September 11, 2009Date of Patent: November 19, 2013Assignees: Mie University, Takara Bio Inc.Inventors: Hiroshi Shiku, Hiroaki Ikeda, Jun Mitsui, Yuki Takenaka, Junichi Mineno, Ikunoshin Kato
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Publication number: 20130115199Abstract: A polypeptide comprising a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 5 of Sequence Listing or a polypeptide consisting of an amino acid sequence having deletion, addition, insertion or substitution of one to several amino acid residues in the sequence, the polypeptide being capable of constituting an HLA-A24-restricted, MAGE-A4143-151-specific T cell receptor together with a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 2 of Sequence Listing.Type: ApplicationFiled: January 4, 2013Publication date: May 9, 2013Applicants: MIE UNIVERSITY, TAKARA BIO INC.Inventors: Takara Bio Inc., Mie University
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Publication number: 20130102048Abstract: A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.Type: ApplicationFiled: October 12, 2012Publication date: April 25, 2013Applicant: TAKARA BIO INC.Inventor: TAKARA BIO INC.
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Publication number: 20130065281Abstract: A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.Type: ApplicationFiled: May 9, 2011Publication date: March 14, 2013Applicant: TAKARA BIO INC.Inventors: Yuko Nakabayashi, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Patent number: 8383401Abstract: A polypeptide comprising a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 5 of Sequence Listing or a polypeptide consisting of an amino acid sequence having deletion, addition, insertion or substitution of one to several amino acid residues in the sequence, the polypeptide being capable of constituting an HLA-A24-restricted, MAGE-A4143-151-specific T cell receptor together with a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 2 of Sequence Listing.Type: GrantFiled: June 23, 2011Date of Patent: February 26, 2013Assignees: MIE University, Takara Bio Inc.Inventors: Hiroshi Shiku, Atsunori Hiasa, Satoshi Okumura, Hiroaki Naota, Yoshihiro Miyahara
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Patent number: 8367328Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.Type: GrantFiled: October 15, 2008Date of Patent: February 5, 2013Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
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Patent number: 8349569Abstract: A method for measuring a fibronectin fragment which is easy to handle and has excellent measuring accuracy, specificity and reproducibility is provided. An anti-fibronectin fragment monoclonal antibody which reacts with a human fibronectin fragment but does not react with human fibronectin, a measuring reagent containing the monoclonal antibody, a method for measuring a fibronectin fragment which uses the monoclonal antibody and a hybridoma which produces the monoclonal antibody are provided.Type: GrantFiled: March 31, 2010Date of Patent: January 8, 2013Assignee: Takara Bio Inc.Inventors: Chika Kato, Yuka Sano, Kyoko Kamihagi, Ikunoshin Kato