Patents Assigned to Takara Bio, Inc.
  • Method for cultivating hon-shimeji mushroom on fungal bed
    Patent number: 7971388
    Abstract: According to the present invention, there is provided a fungal bed cultivation method of a hon-shimeji mushroom, wherein induction of primordia of a fruit body is conducted by illumination under incubation conditions, and an ongoing culture for fungal bed cultivation of a hon-shimeji mushroom, wherein primordia of a fruit body have been formed thereon during incubation. The culture medium is maintained in a clean state and it is advantageous because the formation of primordia of a fruit body can be carried out in a clean environment and the culture medium wherein primordia of a fruit body have been formed can be transferred to distant mushroom cultivation facilities under clean conditions as such.
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: July 5, 2011
    Assignee: Takara Bio Inc.
    Inventors: Takashi Kawai, Katsuhiko Kusakabe, Akihiko Kita, Ikunoshin Kato
  • METHOD FOR PRODUCTION OF PLURIPOTENT STEM CELL
    Publication number: 20110117653
    Abstract: The present invention relates to a method for production of a cell population containing a pluripotent stem cell, said method comprising a step of treating a somatic cell which has been contacted with nuclear reprogramming factors under nutrient-starved condition, and/or a step of treating the somatic cell with an agent capable of arresting cell cycle. The present invention allows induction and growth of pluripotent stem cells at high frequency, and it also allows production of pluripotent stem cells with high efficiency. The nuclear reprogramming factors to be used may be any selected from the group consisting of OCT4, SOX2, c-MYC, KLF4, NANOG and LIN28.
    Type: Application
    Filed: July 7, 2009
    Publication date: May 19, 2011
    Applicant: Takara Bio Inc.
    Inventors: Tatsuji Enoki, Fumiko Iwamoto, Toshikazu Nishie, Takahiro Marui, Fuyuko Takashima, Ikunoshin K
  • Thermostable ribonuclease H from
    Patent number: 7932071
    Abstract: A polypeptide having an RNaseH activity from Archaeoglobus profundus and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: April 26, 2011
    Assignee: Takara Bio Inc.
    Inventors: Shigekazu Hokazono, Takashi Uemori, Tetsuki Tanaka, Ikunoshin Kato
  • Method of extended culture for antigen-specific cytotoxic lymphocytes
    Patent number: 7910368
    Abstract: The present invention is a method for inducing cytotoxic T cell having an antigen-specific cytotoxic activity, a method for maintaining the cell, a method for continuously culturing the cell or a method for expanding the cell, comprising the step of culturing a cytotoxic T cell in the presence of at least one substance selected from the group consisting of (A) a substance having a binding activity to CD44; (B) a substance capable of regulating a signal emitted by binding a CD44 ligand to CD44; (C) a substance capable of inhibiting binding of a growth factor to a growth factor receptor; (D) a substance capable of regulating a signal emitted by binding of a growth factor to a growth factor receptor; and (E) fibronectin, a fragment thereof or a mixture thereof.
    Type: Grant
    Filed: August 15, 2002
    Date of Patent: March 22, 2011
    Assignee: Takara Bio Inc.
    Inventors: Hiroaki Sagawa, Mitsuko Ideno, Ikunoshin Kato
  • METHOD FOR PRODUCTION OF TRANSFECTED CELL
    Publication number: 20110027242
    Abstract: Disclosed is a method for producing a cell population into which a desired gene is introduced, which is characterized by comprising the following steps (1) and (2): (1) culturing a cell population containing a T-cell and/or a progenitor cell of a T-cell in a vessel containing fibronectin or a fragment thereof and a CD3 ligand; and (2) adding a vector carrying the desired gene to the vessel of the step (1). By carrying out both the steps (1) and (2) in the same vessel, the gene transfer can be achieved with high efficiency.
    Type: Application
    Filed: March 27, 2009
    Publication date: February 3, 2011
    Applicant: TAKARA BIO INC.
    Inventors: Ikuei Nukaya, Yumi Goto, Yasuhiro Tosaka, Junichi Mineno, Ikunoshin Kato
  • ACTIVITY ENHANCER FOR DETOXIFYING ENZYME
    Publication number: 20100317613
    Abstract: It is intended to provide a drug, a food or a feed which has an effect of enhancing the activity of a second-phase detoxifying enzyme and an effect of increasing intracellular glutathione content.
    Type: Application
    Filed: October 11, 2007
    Publication date: December 16, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Hiromu Ohnogi, Yoko Kudo, Hiroko Nakahara, Tatsuji Enoki, Ikunoshin Kato
  • Method for enhancing polymerase activity
    Patent number: 7846703
    Abstract: A polymerase activity is effectively enhanced by adding an anionic surfactant, in particular an anionic surfactant having a polyethoxyl group, to a reaction mixture containing a polymerase.
    Type: Grant
    Filed: October 2, 2007
    Date of Patent: December 7, 2010
    Assignee: Takara Bio Inc.
    Inventors: Eiji Kobayashi, Yuki Ueda, Yoshimi Sato, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Method of extensive culture of antigen-specific cytotoxic T cells
    Patent number: 7816134
    Abstract: The present invention provides methods for inducing, maintaining and expanding CTL (cytotoxic T cell) having an antigen-specific cytotoxic activity at a high level, which is useful in the adoptive immunotherapy, by using as an effective ingredient at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides, and salts thereof. The above-mentioned compounds include fucoidans, heparins, alginic acid, chondroitin sulfate A, chondroitin sulfate B, pectic acid, hyaluronic acid, degradation products of fucoidans, sulfated glucose, sulfated fucose and salts thereof.
    Type: Grant
    Filed: August 15, 2001
    Date of Patent: October 19, 2010
    Assignee: Takara Bio Inc.
    Inventors: Hiroaki Sagawa, Mitsuko Ideno, Ikunoshin Kato
  • Anti-Fibronectin Fragment Monoclonal Antibody
    Publication number: 20100248262
    Abstract: A method for measuring a fibronectin fragment which is easy to handle and has excellent measuring accuracy, specificity and reproducibility is provided. An anti-fibronectin fragment monoclonal antibody which reacts with a human fibronectin fragment but does not react with human fibronectin, a measuring reagent containing the monoclonal antibody, a method for measuring a fibronectin fragment which uses the monoclonal antibody and a hybridoma which produces the monoclonal antibody are provided.
    Type: Application
    Filed: March 31, 2010
    Publication date: September 30, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Chika KATO, Yuka SANO, Kyoko KAMIHAGI, Ikunoshin KATO
  • Enhancement of retroviral gene transduction employing polypeptides comprising the fibronectin heparin II binding domain
    Patent number: 7790849
    Abstract: An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.
    Type: Grant
    Filed: September 30, 2008
    Date of Patent: September 7, 2010
    Assignee: Takara Bio Inc.
    Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
  • Endoribonuclease
    Patent number: 7745190
    Abstract: A novel endoribonuclease activity exhibiting polypeptide; a nucleic acid coding for the polypeptide; a recombinant DNA comprising the nucleic acid; a transformant obtained by transformation using the recombinant DNA; a process for producing the polypeptide, characterized by including the steps of culturing the transformant and collecting the polypeptide from the culture; a process for producing single-strand RNA fragments, characterized by including the step of causing the polypeptide to act on a single-strand RNA; and a method of fragmenting a single-strand RNA.
    Type: Grant
    Filed: September 8, 2006
    Date of Patent: June 29, 2010
    Assignee: Takara Bio Inc.
    Inventors: Masamitsu Shimada, Masanori Takayama, Kiyoza Asada, Ikunoshin Kato
  • Method of Producing Lymphocytes
    Publication number: 20100150886
    Abstract: A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.
    Type: Application
    Filed: August 10, 2006
    Publication date: June 17, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Takahiro Marui, Kinuko Magamine, Nobuke Muraki, Akiko Kato, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
  • FUNGAL BED CULTIVATION METHOD OF HON-SHIMEJI MUSHROOM
    Publication number: 20100139157
    Abstract: A fungal bed cultivation method of a hon-shimeji mushroom, in which the sprouting step and/or fruit body growing step is carried out under an environmental condition of high CO2 concentration, is provided. Examples of the environmental condition of high CO2 concentration include a CO2 concentration of 2,500 ppm or more in the sprouting step and a CO2 concentration of 5,000 ppm or more in the fruit body growing step. Since the formation ratio of a budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved by embodiments of the present invention, stable production of a hon-shimeji mushroom by its large scale commercial cultivation becomes possible.
    Type: Application
    Filed: November 30, 2009
    Publication date: June 10, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Takashi Kawai, Mayu Hashimoto, Takeshi Sugimori, Katsuhiko Kusakabe, Akihiko Kita, Ikunoshin Kato
  • POLYPEPTIDES HAVING DNA POLYMERASE ACTIVITY
    Publication number: 20100143979
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Application
    Filed: February 18, 2010
    Publication date: June 10, 2010
    Applicant: Takara Bio Inc.
    Inventors: Yoshimi SATO, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • DRUGS, FOODS OR DRINKS WITH THE USE OF ALGAE-DERIVED PHYSIOLOGICALLY ACTIVE SUBSTANCES
    Publication number: 20100137228
    Abstract: Medicinal compositions for treating, ameliorating or preventing diseases with sensitivity to 3,6-anhydrogalactopyranose represented by formula (1): foods, drinks, cosmetics, etc. containing as the active ingredient at least one member selected from the group consisting of the above-mentioned compound, its aldehyde, its hydrate and 2-O-methylated derivatives thereof and soluble sugar compounds containing the above compound. This compound also shows, for example, an apoptosis-inducing activity, a carcinostatic activity and inhibitory activities on the production of active oxygen, lipid peroxide radicals and NO, which makes it useful also as the active ingredient of antioxidants and preservatives.
    Type: Application
    Filed: August 11, 2008
    Publication date: June 3, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Tatsuji ENOKI, Hiroaki SAGAWA, Takanari TOMINAGA, Eiji NISHIYAMA, Nobuto KOYAMA, Takeshi SAKAI, Fu-Gong YU, Katsushige IKAI, Ikunoshin KATO
  • VECTOR FOR GENE THERAPY
    Publication number: 20100137415
    Abstract: The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections.
    Type: Application
    Filed: April 16, 2008
    Publication date: June 3, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Hideto Chono, Kazuya Matsumoto, Hajima Matsumura, Junichi Mineno, Ikunoshin Kato
  • PROCESS FOR PRODUCING OSTEOCALCIN-CONTAINING EXTRACT
    Publication number: 20100105867
    Abstract: A process for producing an extract containing activated osteocalcin; an extract which can be obtained by this production process; a food, a drink, a drug, an oral cavity-care composition and a feed containing this extract; and a growth enhancer containing osteocalcin originating in a bone.
    Type: Application
    Filed: March 5, 2008
    Publication date: April 29, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Hiromu Ohnogi, Shinichi Adachi, Kazuo Nakagawa, Hiroki Iwamoto, Ikunoshin Kato
  • Polypeptides having DNA polymerase activity
    Patent number: 7704713
    Abstract: A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
    Type: Grant
    Filed: May 12, 2005
    Date of Patent: April 27, 2010
    Assignee: Takara Bio Inc.
    Inventors: Yoshimi Sato, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
  • Method for Production of T Cell Population
    Publication number: 20100068192
    Abstract: A method for preparing a T cell population, wherein the T cell population expresses CD45RA and expresses at least one selected from the group consisting of CD62L, CCR7, CD27, and CD28, characterized in that the method comprises the step of culturing a cell population comprising a T cell, in the presence of fibronectin, a fragment thereof or a mixture thereof.
    Type: Application
    Filed: September 27, 2006
    Publication date: March 18, 2010
    Applicant: TAKARA BIO INC.
    Inventors: Tatsuji Enoki, Akiko Kato, Mitsuko Ideno, Hiroaki Sagawa, Ikunoshin Kato
  • Fucoidan-containing cosmetics
    Patent number: 7678368
    Abstract: To find a substance which has a skin cosmeticizing effect such as prevention of skin aging, amelioriation of sensitive skin, or antipruritic action, and can serve as an effective ingredient for a hair-care product, thereby providing cosmetics comprising the effective ingredient. Concretely, there are provided cosmetics characterized in that the cosmetics comprise as an effective ingredient a compound selected from a fucoidan, a degradation product thereof, a sulfated monosaccharide or a salt thereof.
    Type: Grant
    Filed: December 20, 2005
    Date of Patent: March 16, 2010
    Assignee: Takara Bio Inc.
    Inventors: Shigetoshi Mizutani, Suzu Deguchi, Eiji Kobayashi, Eiji Nishiyama, Hiroaki Sagawa, Ikunoshin Kato