Abstract: Disclosed is a method for making full-length CDNA libraries, which is for making libraries of cDNAs having a length corresponding to a full-length of mRNAs and comprises the following steps of; binding a tag molecule to a diol structure present in 5′ Cap (7MeGpppN) sites of mRNAs, forming RNA-DNA hybrids by reverse transcription using primers such as oligo dT and the mRNAs connected with the tag molecule as templates, and separating RNA-DNA hybrids carrying a DNA corresponding to a full-length of mRNAs from the RNA-DNA hybrids formed above by using function of the tag molecule. To obtain mRNA connected with a tag molecule, the diol structure present in 5′ Cap site of mRNA is subjected to a ring-open reaction by oxidation with sodium periodate to form a dialdehyde and the dialdehyde is reacted with a tag molecule having a hydrazine terminus.

Abstract: A magnet for producing a static magnetic field is improved, and a small-sized, high-resolution nuclear magnetic resonance system is provided. A superconductive bulk (17) of a high-temperature superconductor is cooled in a vacuum insulated container (16), and magnetized with a magnetizing coil (19). This is used for analyzing a subject (11).

Abstract: A semi-conductive grindstone (10) comprising grains and a semi-conductive binder to fix the grains is prepared, a voltage is applied between the grindstone and the conductive workpiece (1), an conductive grinding fluid is supplied between them, the grindstone is contacted to the workpiece, the binder of the grindstone is subjected to electrolytic dressing in the contact point, and the workpiece is simultaneously ground by using the grindstone. A semi-conductive binder is preferably composed of metal powder and an insulating resin. Consequently, application is possible to peculiar grindstones such as ball-nose grindstone, grinding of the workpiece is simultaneously possible with dressing of the working surface of the grindstone by electrolytic dressing, and thus, grinding of long duration is also possible maintaining high efficiency and high preciseness.

Abstract: An X-ray generating apparatus in which X-rays are emitted from laser plasma, the X-ray generating apparatus including a strong magnetic field generating device for generating a magnetic field component substantially parallel with the target surface in the vicinity of the laser plasma. The magnetic field component is arranged to generate a magnetic force which acts directly on charged particles in the laser plasma to bend the tracks of the charged particles, causing the charged particles to be confined in a magnetic field formed by the magnetic field component. The magnetic flux of the strong magnetic field is directed to a direction which is different from the direction in which the laser plasma is generated. An X-ray supply object is disposed in the laser plasma generating direction. Charged particles, liable to be directed to the X-ray supply object, are mainly confined in the strong magnetic field.

Abstract: (A) Applying a voltage between an electrically conductive grindstone (1) and a shaping electrode (4) for shaping the grindstone, generating a spark on a contact point by contacting the shaping electrode to the grindstone, thereby shaping the grindstone by the spark, and (B) simultaneously applying a voltage between the grindstone and a dressing electrode (2) for dressing the grindstone that is oppositely aligned to the grindstone without contact, supplying the electrically conductive grinding fluid between them, thereby dressing the grindstone by electrolytic dressing. According to these steps, highly efficient and simultaneous processing is achieved to provide a high precision profiling high quality mirror.

Abstract: Disclose is a method for making full-length cDNA libraries, which is for making libraries of cDNAs having lengths corresponding to full lengths of mRNAs and comprises the following steps of; forming RNA-DNA hybrids by reverse transcription starting from primers using mRNAs as templates, chemically binding a tag molecule to a diol structure present in the 5' Cap (.sup.7Me G.sub.ppp N) site of a mRNA which is forming a RNA-DNA hybrid, and separating RNA-DNA hybrids carrying a DNA corresponding to a full-length mRNA from the RNA-DNA hybrids formed above by using a function of the tag molecule. The present method is a method for preparing full-length cDNA libraries utilizing a method for labeling the 5' Cap site more efficiently than protein enzyme reactions, which is avoidable a decrease of a full-length cDNA synthesis efficiency caused by cleavage of mRNA, and can synthesize a full-length cDNA more efficiently.

Abstract: A photo-reactive grinding wheel 1 is irradiated with light by a light irradiation device 2 which is provided opposite to the grinding wheel, to bring about a chemical reaction and change in property, and dissolved/removed by a solution 4. Simultaneously, a workpiece 5 is processed by the photo-reactive grinding wheel 1. Thus, processing can be performed without causing clogging in the grinding wheel of a resin bond containing fine abrasive grains, high-grade surface roughness can be realized, and processing efficiency is relatively high. The controllability of the dressing is excellent, automation of dressing and in-process dressing can also be realized, a system which contains no metal ion in the whole processing can be designed, an expensive device is not required, and handling is easy.

Abstract: After a sample stage moves and brings a sample titer plate toward and into contact with a capillary array end, a high voltage is applied across electrodes for a prescribed time for injecting samples. Thereafter an electrophoresis reservoir comes into contact with the capillary array end, for starting electrophoresis. When electrophoresed/separated DNA fragments pass through a detection part, an excitation/photoreceiving optical system is scanned so that photomultipliers detect fluorescence from four types of fluorescent materials labeling the samples. The excitation/photoreceiving optical system comprises an epi-optical system and a confocal optical system, and is scanned at a high speed.

Abstract: There is disclosed an electrode 10 generating hydro-dynamic pressure for electrolytic dressing grinding. The electrode has a plurality of narrow portions 11 having constant gaps from a processed surface 1a of a grinding wheel 1, and a plurality of concave portions 12 each disposed between the narrow portions and having a gap wider than the narrow portion. A section of flow path (gap) formed between the grinding wheel 1 and the electrode 10 becomes concave/convex along a moving direction of the grinding wheel 1. When the liquid repeatedly flows through the concave/convex gap, dynamic and static pressures generated in the gap largely fluctuate. By the fluctuation, the adhesion of metal deposits to the narrow portions 11 is reduced, and the concave portions 12 form pockets, so that the electrolytic liquid can be stably supplied and the inclusion of air can be reduced.

Abstract: A base plate is made of an insulating material, and comprised of a flat surface and a connector part connected therewith. A plurality of wells are vertically and transversely arranged on the surface of the base plate at regular intervals respectively, and the respective wells are provided with individual electrode patterns reaching the connector part from bottoms thereof through the surface of the base plate. The connector part is connected to an external high-voltage application part.

Abstract: A method for judging a possibility of the onset of bovine leukemia caused by bovine leukemia virus BLV, wherein a bovine individual, in which an amino acid sequence defined by the amino acid numbers 75 to 78 of the .beta.1 domain of the bovine MHC Class II DR.beta. chain is Val-Asp-Thr-Tyr, is judged to have a possibility of the onset of the leukemia: and a method for judging a resistance to the onset of bovine leukemia caused by the bovine leukemia virus BLV, wherein a bovine individual, in which an amino acid defined by the amino acid number 78 of the .beta.1 domain of the bovine MHC Class II DR.beta. chain is Val, is judged to have a resistance to the onset of the leukemia.

Abstract: A variably sound-absorbing device comprises a piezoelectric material 32 the peripheral portion of which is fixed to a frame 31 or the like, at least one pair of electrodes 34 formed on opposite surfaces of the piezoelectric material, and at least one circuit element 36 through which the electrodes are connected to each other. The piezoelectric material 32 is boardlike and curved, and an electrical characteristic of the circuit element 36 (a circuit showing a negative capacitance for example) is variable. The modulus of elasticity (the real number part of the modulus of elasticity) and the loss factor (the imaginary number part of the modulus of elasticity) of the piezoelectric material are thereby varied and the sound-absorbing characteristic is electrically varied to a considerable extent.

Abstract: Disclosed is a method for determining a nucleotide sequence of DNA product amplified by polymerase chain reaction not requiring removal of primers and/or 2'-deoxyribonucleoside-5'-triphosphates and/or derivatives thereof, which comprises reacting ribonucleoside-5'-triphosphates comprising ATP, GTP, CTP, UTP and derivatives thereof and one or more of 3'-deoxyribonucleotide-5'-triphosphates comprising 3'-dATP, 3'-dGTP, 3'-dCTP, 3'-dUTP and derivatives thereof in the presence of an RNA polymerase and a DNA product which has been amplified by polymerase chain reaction and contains a promoter sequence for the RNA polymerase to afford a nucleic acid transcription product, separating the obtained nucleic acid transcription product and determining a nucleic acid sequence from the resluting separated fractions.

Abstract: In a free form machining tool 10 for machining a surface to be machined while bringing the lower end into contact with the surface to be machined by a rotation around an axis z, the tool comprises a spherical tool 12 having a spherical surface machining section 13 on the lower portion, and a bearing 14 for supporting the spherical tool by an axis a of rotation which passes through the center .largecircle. of the spherical surface and is different from the axis. The tool also includes rotating means 16 (for example, a turbine) for rotating the spherical tool around the axis of rotation. Thus, even if the spherical surface machining section comes into contact with the surface to be machined on the axis (radius: 0) of the tool, the spherical surface machining section is rotated around the axis a of rotation which is different from the axis z. Therefore, a peripheral speed of the axis is not zero (0).

Abstract: The subject invention provides a novel ceramide glucosyltransferase having catalytic activity of glucose transfer from UDP-Glc to ceramide, and a nucleic acid sequence encoding the ceramide glucosyltransferase.

Abstract: The methods and apparatuses for the control of neutron beams are herewith presented. Through the application of the methods and apparatuses presented one can manipulate various characteristics of neutron beams such as shape, velocity, density, polarization and other traits. In general three sequential operations are performed on the neutron beam, although variations of these steps are described to suit various purposes. First, a neutron beam is passed through a gradient magnet field which causes rotation of the beam in phase space. Second, the spin direction of a neutron beam is reversed through the application of a spin flipper. Third, the neutron beam is compressed in the longitudinal direction of the neutron beam in phase space. This produces a neutron beam having small divergence in phase space. The resultant neutron beam corresponds to a thin dense beam in real space. Variations of this paradigm allow for the manipulation of many characteristics of neutron beams to suit ones purpose.

Abstract: A medicament for preventive and therapeutic treatment of obesity and a disease with abnormal lipid metabolism which comprises as an active ingredient a compound selected from the group consisting of 24-alkylcholestan-3-ones and 24-alkylcholesten-3-ones such as 24-alkylcholestan-3-ones, 24-alkylcholestmonoen-3-ones, 24-alkylcholestdien-3-ones, 24-alkylcholesttrien-3-ones and 24-alkylcholesttetraen-3-ones.

Abstract: A polypeptide characterized by amino acid Nos. 1-789 of the amino acid sequence defined by SEQ ID NO:1 of the sequence listing or a polypeptide having the said amino acid sequence incorporated by insertion, deletion, or substitution of one or more amino acid residues. The peptides have catalytic activities of converting copalyl pyrophosphate to ent-kaurene and are useful for the regulation of plant growth. Also provided are DNAs encoding the polypeptides.

Abstract: The present invention provides a nucleotide sequence encoding Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase and an enzymatically active fragment thereof. The present invention also provides a nucleotide sequence encoding an extracellularly releasable protein capable of catalyzing Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransfer which comprises an enzymatically active fragment of the Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase together with a nucleotide sequence encoding at least one signal peptide. The present invention also provides recombinant vectors comprising nucleotide sequences encoding Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase. Processes for preparing extracellularly releasable polypeptide fragments of Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase are provided as well.

Abstract: The elastic modulus of a piezoelectric substance is controlled over a wide range using a simple method. The effective modulus (the real part of the elastic modulus) and the energy loss modulus (the imaginary part of the elastic modulus) of the piezoelectric substance are changed by installing electrodes on the surface of the piezoelectric substance and by connecting a mechanism consisting of one or more circuit elements to each of the electrodes.