Abstract: In some embodiments, an apparatus, system, and method for activating a low-adhesion state of a thermal-sensitive tape is described. An example apparatus embodiment includes a light source and a temperature sensor. The light source is configured to illuminate a target area of the thermal-sensitive tape with a first spectrum of electromagnetic radiation to provide heating of the target area. The first spectrum including a first wavelength outside of a visible spectrum. The temperature sensor is configured to detect a second spectrum of electromagnetic radiation to approximate a temperature of the target area. The second spectrum includes a second wavelength different than the first wavelength.
Abstract: Disclosed are methods and related compositions for genomic editing. In one aspect, methods of editing double stranded DNA (dsDNA) use first and second editing complexes specific for first and second target sequences on the sense and antisense strands of the dsDNA molecule, respectively. Each editing complex comprises an extended guide RNA associated with a fusion editor protein, which comprises a functional nickase domain and a functional reverse transcriptase domain. The respective guide RNAs guide their associated fusion editor proteins to the dsDNA, which implement single stranded breaks on opposite strands of the dsDNA. The respective reverse transcriptase domains generate 3? overhangs. Repair of the dsDNA excises the portion of dsDNA disposed between the two single-stranded breaks. A variety of configurations and applications of the method are disclosed, providing flexible, facile, efficient, and precise methods to impose genetic manipulations.
Type:
Application
Filed:
November 4, 2021
Publication date:
January 11, 2024
Applicant:
University of Washington
Inventors:
Jay Ashok Shendure, Wei Chen, Junhong Choi
Abstract: The application discloses multimeric assemblies packaging one or more active component and their use to carry out nucleic acid regulation or gene editing.
Type:
Grant
Filed:
February 12, 2018
Date of Patent:
January 9, 2024
Assignee:
UNIVERSITY OF WASHINGTON
Inventors:
Marc Joseph Lajoie, Elizabeth Erin Gray, Neil P. King, Daniel Brewster Stetson, David Baker, Katherine DaPron
Abstract: A method for determining a presence of arthritis in a patient, including obtaining a first image of a patient's joint, wherein the first image is a visible light image, obtaining a second image of the patient's joint, wherein the second image is a thermal light image, determining an outline of the patient's joint from the first image, determining an outline of a reference area from the first image, wherein the patient's joint is adjacent to the reference area, determining a first representative topological temperature within the outline of the patient's joint of the first image from the second image, determining a second representative topological temperature within the outline of the reference area of the first image from the second image, comparing the first representative topological temperature and the second representative topological temperature; and determining a likelihood of the presence of arthritis within the patient's joint.
Type:
Application
Filed:
June 28, 2023
Publication date:
January 4, 2024
Applicants:
Seattle Children's Hospital dba Seattle Children's Research Institute, University of Washington
Inventors:
Yongdong Zhao, Joshua Scheck, Savannah Corrina Partridge, Ramesh S. Iyer, Mahesh Thapa, Debosmita Biswas, Sr., Nivrutti Vasudev Bhide, Kevin Charles Cain, Jason Michael Pyke
Abstract: Systems and methods for capturing and rendering light fields for head-mounted displays are disclosed. A mediated-reality visualization system includes a head-mounted display assembly comprising a frame configured to be mounted to a user's head and a display device coupled to the frame. An imaging assembly separate and spaced apart from the head-mounted display assembly is configured to capture light-field data. A computing device in communication with the imaging assembly and the display device is configured to receive light-field data from the imaging assembly and render one or more virtual cameras. Images from the one or more virtual cameras are presented to a user via the display device.
Type:
Application
Filed:
March 23, 2023
Publication date:
January 4, 2024
Applicant:
University of Washington
Inventors:
Joshua R. Smith, Samuel R. Browd, Rufus Griffin Nicoll, James Andrew Youngquist
Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
Type:
Application
Filed:
August 24, 2023
Publication date:
January 4, 2024
Applicant:
University of Washington
Inventors:
Georg Seelig, Richard Muscat, Alexander B. Rosenberg
Abstract: High intensity focused ultrasound systems for treating tissue are disclosed herein. A system of treating tissue in a patient in accordance with an embodiment of the present technology can include, for example, an ultrasound source having a focal region and configured to deliver high intensity focused ultrasound energy to a target site in tissue of the patient. The system can further include a controller operably coupled to the ultrasound source. The controller comprises a pulsing protocol for delivering the high intensity focused ultrasound energy with the ultrasound source to the target site. The controller is configured to cause the ultrasound source to pulse high intensity focused ultrasound waves to lyse cells in a volume of the tissue of the subject while preserving an extracellular matrix in the volume of the tissue exposed to the high intensity focused ultrasound waves.
Type:
Grant
Filed:
July 13, 2020
Date of Patent:
January 2, 2024
Assignee:
University of Washington
Inventors:
Yak-Nam Wang, Michael R. Bailey, Tatiana D. Khokhlova, Wayne Kreider, Adam D. Maxwell, George R. Schade, Vera A. Khokhlova
Abstract: Compounds and compositions for inhibiting miR-21, methods for inhibiting miR-21 in a subject, and methods for treating a disease or condition treatable by decreasing the level of microRNA miR-21 in a subject.
Abstract: A nanoparticle for targeted siRNA delivery comprising an iron oxide core and a chitosan-polyethylene glycol-polyethylenimine copolymer coating surrounding the core, chlorotoxin covalently coupled to the coating, and an siRNA reversibly associated to the coating by non-covalent interaction. Methods for making the nanoparticle and methods for using the nanoparticle to suppress the expression of O6-methylguanine-DNA methyltransferase (MGMT), treating brain cancer, and killing cancer stem cells.
Type:
Application
Filed:
October 6, 2021
Publication date:
December 21, 2023
Applicant:
University of Washington
Inventors:
Miqin Zhang, Richard G. Ellenbogen, Kui Wang, Forrest M. Kievit, John R. Silber, Zachary Stephen, Peter A. Chiarelli
Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.
Type:
Application
Filed:
November 23, 2022
Publication date:
December 21, 2023
Applicant:
University of Washington through its Center for Commercialization
Inventors:
Daniel T. Chiu, Changfeng Wu, Xuanjun Zhang, Jiangbo Yu, Fangmao Ye
Abstract: The present disclosure describes a method for preparing a paper nanocomposite, including: continuously providing a first suspension that includes lignocellulosic pulp fibers, cellulose nanofibrils, carbon nanotubes, and a cationic surfactant; continuously adding a second suspension to the first suspension to provide a slurry, the second suspension includes lignocellulosic pulp fibers, cellulose nanofibrils, carbon nanotubes, and an anionic surfactant; depositing the slurry comprising the first and second suspensions onto the substrate; and dewatering the slurry to form the paper nanocomposite.
Abstract: Systems and methods for optical computation and acousto-optic modulation are described. The systems and methods comprise acousto-optic modulators with reflectors and transducers used for actuation and modulation of mechanical waves. The systems further comprise multilayer optical computing systems incorporating arrays of acousto-optic modulators.
Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
Type:
Application
Filed:
August 24, 2023
Publication date:
December 14, 2023
Applicant:
University of Washington
Inventors:
Georg Seelig, Richard Muscat, Alexander B. Rosenberg
Abstract: Disclosed herein are embodiments of a hydrothermal reactor, such as a downflow hydrothermal reactor and methods of using the same. Also disclosed herein are system embodiments comprising the hydrothermal reactor. Method embodiments disclosed herein facilitate determining operation parameters for the hydrothermal reactor that give rise to efficient feedstock conversion to products while maintaining integrity of the reactor (e.g., avoiding corrosion) and providing safe operating conditions. The disclosed reactor and system embodiments facilitate situations where small scale and/or remote destruction of feedstocks (e.g., chemical warfare agents and/or environmental toxins) is needed.
Type:
Application
Filed:
October 27, 2021
Publication date:
December 14, 2023
Applicant:
University of Washington
Inventors:
Igor V. Novosselov, Brian Pinkard, Stuart Moore
Abstract: Disclosed are organic electrooptic materials. The electrooptic materials are functionalized by novel methods and structures. The electrooptic materials are structured with a head and a tail where the head and tail sequentially assemble into a non-centrosymmetric ordered array. The electrooptic materials are further structured in covalently bonded dimer pairs where one of the pairs is zwitterionic. The non-centrosymmetric ordered array bonds directly to electrodes which allows for efficient application of the electric field. The organic electrooptic materials disclosed offer greater hyperpolarizability, greater bandwidth, reduced operating voltage with less optical loss.
Type:
Application
Filed:
June 13, 2023
Publication date:
December 14, 2023
Applicants:
University of Washington, NLM Photonics
Inventors:
Delwin L. Elder, Larry R. Dalton, III, Lewis E. Johnson, Scott R. Hammond, Bruce H. Robinson, Kevin M. O'Malley
Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
Type:
Application
Filed:
August 24, 2023
Publication date:
December 7, 2023
Applicant:
University of Washington
Inventors:
Georg Seelig, Richard Muscat, Alexander B. Rosenberg
Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
Type:
Application
Filed:
August 24, 2023
Publication date:
December 7, 2023
Applicant:
University of Washington
Inventors:
Georg Seelig, Richard Muscat, Alexander B. Rosenberg
Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.
Type:
Grant
Filed:
February 15, 2022
Date of Patent:
December 5, 2023
Assignees:
University of Washington, Clemson University Research Foundation
Inventors:
Daniel T. Chiu, Changfeng Wu, Jason McNeill, Jiangbo Yu