Abstract: The present invention provides recombinant adenoviral compositions and methods for their use in treating disorders associated with epithelial tissues.
Abstract: Disclosed herein are designed heterodimer proteins, monomeric polypeptides capable of forming heterodimer proteins, protein scaffolds including such polypeptides, and methods for using the heterodimer proteins and subunit polypeptides for designing logic gates.
Type:
Grant
Filed:
November 4, 2019
Date of Patent:
November 21, 2023
Assignee:
UNIVERSITY OF WASHINGTON
Inventors:
Zibo Chen, Scott Boyken, Sherry Bermeo, Robert A. Langan, David Baker
Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.
Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.
Type:
Grant
Filed:
September 20, 2021
Date of Patent:
November 21, 2023
Assignee:
UNIVERSITY OF WASHINGTON
Inventors:
David Baker, David Younger, Eric Klavins
Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.
Type:
Grant
Filed:
July 6, 2021
Date of Patent:
November 21, 2023
Assignee:
University of Washington
Inventors:
Daniel T. Chiu, Jiangbo Yu, Yu Rong, Changfeng Wu
Abstract: Examples of systems and methods are described for detecting heart beats of a subject. The systems and methods may be based on motion of the subject due to cardiac activity, and may operate without contact with the subject. Example systems may provide an interrogation signal to the subject. Reflected signals from the subject are incident on a microphone array. The reflected signals may be processed and beamformed using a set of beamforming weights. The beamforming weights may be selected in a manner to reduce components of the reflected signals due to breathing motion of the subject while increasing the relative contribution of the reflected signals due to cardiac activity. The beamformed signal may provide a waveform indicative of heart beats. Inter-beat intervals, heart rates, and/or other health metrics may be calculated based on the waveform.
Type:
Application
Filed:
September 24, 2021
Publication date:
November 16, 2023
Applicant:
University of Washington
Inventors:
Shyamnath Gollakota, Anran Wang, Arun R. Mahankali Sridhar
Abstract: Fluidic devices and methods for autonomous droplet generation and methods for droplet manipulation are described. In an embodiment, the fluidic device comprises a substrate defining: an inlet reservoir shaped to receive and to carry a carrier liquid; a converging region in fluidic communication with the inlet reservoir and shaped to receive a liquid sample; a constriction adjacent to and in fluidic communication with the converging region, wherein the constriction defines a pathway configured to allow passage of fluid therethrough; a diverging region in fluidic communication with and downstream of the constriction; and an outlet reservoir in fluidic communication with the diverging region, wherein the fluidic device does not comprise a portion covering the outlet reservoir opposite the substrate.
Type:
Application
Filed:
May 9, 2023
Publication date:
November 16, 2023
Applicant:
University of Washington
Inventors:
Jian Wei Khor, Ashleigh Brooks Theberge, Erwin Stefan Peter Berthier, Ulri Nicole Lee, Jean Berthier, Tristan Marriner Nicholson
Abstract: The invention provides isolated primate cells preferably human cells that comprise a genetically engineered disruption in a beta-2 microglobulin (B2M) gene, which results in deficiency in MHC class I expression and function. Also provided are the method of using the cells for transplantation and treating a disease condition.
Abstract: An aerial robot system may include an aerial robot having an airframe, a piezo actuator, a wing connected to the piezo actuator, and a photovoltaic cell. The system may further include a laser source configured to emit a laser beam oriented toward the photovoltaic cell for conversion by the photovoltaic cell into electrical energy. The aerial robot may further include a boost converter connected to the photovoltaic cell and configured to raise a voltage level of the electrical energy, and a signal generator connected to the boost converter and configured to generate an alternating signal. The piezo actuator is connected to the signal generator to move according to the alternating signal to cause the wing to move in a flapping motion to generate aerodynamic force that moves the robot. Methods for manufacturing aerial robots and corresponding electronics are also disclosed herein.
Type:
Grant
Filed:
May 10, 2019
Date of Patent:
November 14, 2023
Assignee:
UNIVERSITY OF WASHINGTON
Inventors:
Sawyer Fuller, Shyamnath Gollakota, Johannes James, Vikram Subramaniam Iyer, Yogesh M. Chukewad
Abstract: A sample vessel includes a biological sample container and a sample stabilizer container. The biological sample container is configured to receive a biological sample and to store the biological sample. The sample stabilizer container is configured to contain a stabilizer associated with the biological sample. The sample stabilizer container is assembled from a stabilizer vial, an adaptor, and a fluid channel. The stabilizer vial is configured to store an amount of the stabilizer. The adaptor is configured to secure the biological sample container and the stabilizer vial such that the biological sample container and the stabilizer vial form the sample vessel. The fluid channel extends through the adaptor from the stabilizer vial to the biological sample container, the biological sample moving from the biological sample container into the stabilizer vial through the fluid channel.
Type:
Grant
Filed:
June 29, 2021
Date of Patent:
November 14, 2023
Assignee:
University of Washington
Inventors:
Ashleigh Brooks Theberge, Erwin Stefan Peter Berthier, Amanda Haack, Dakota Kennedy, Fang Yun Lim
Abstract: The present disclosure features photon upconversion systems, including a sensitizer and an emitter, wherein the sensitizer absorbs an energy from an incident radiation, and the emitter accepts the energy from the sensitizer via triplet-triplet energy transfer, and emits at a lower wavelength than the incident radiation via a triplet-triplet annihilation process. The present disclosure also features devices including the photon upconversion systems.
Type:
Application
Filed:
September 22, 2021
Publication date:
November 9, 2023
Applicant:
University of Washington
Inventors:
Cody Schlenker, Kathryn Corp, Sarah Pritash, Matthew Rigsby
Abstract: This document provides methods and materials for expanding antigen-specific T cells (e.g., antigen-specific CD4+ T cells and/or antigen-specific CD8+ T cells) in culture. For example, methods and materials for performing a polyclonal stimulation step for a particular duration (e.g., from about 1 hour to about 48 hours) to increase the expansion of T cells having a desired antigen specificity are provided.
Type:
Application
Filed:
July 13, 2023
Publication date:
November 9, 2023
Applicants:
Mayo Foundation for Medical Education and Research, University of Washington
Inventors:
Peter A. Cohen, JR., Sandra J. Gendler, Latha B. Pathangey, Dustin B. McCurry, Jessica E. Gorman, Mary L. Disis
Abstract: Temperature-responsive pressure sensitive adhesives and articles of manufacture, such as bandages and medical tape, incorporating the adhesives are described. Methods for selection of a class of pressure sensitive adhesives for embedding a temperature sensitive polymer into the adhesive formulations are also provided.
Type:
Application
Filed:
September 9, 2021
Publication date:
November 9, 2023
Applicant:
UNIVERSITY OF WASHINGTON
Inventors:
Eric J. Seibel, Leonard Y. Nelson, Christopher R. Fellin, Shawn Swanson, Alshakim Nelson, John Devin MacKenzie
Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.
Type:
Grant
Filed:
September 16, 2022
Date of Patent:
November 7, 2023
Assignee:
UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
Inventors:
Daniel T. Chiu, Mengxia Zhao, Wyatt Nelson, Perry G. Schiro
Abstract: Various noncollimated single photon emission computed tomography (SPECT) technologies are described herein. An example device includes an array of detectors configured to detect a flux of first photons transmitted from a field of view (FOV) over time. The device also includes an attenuator disposed between the array of detectors and the FOV. The attenuator is configured to move over time and to attenuate second photons emitted from the source. In various implementations, the attenuator is not a collimator. Based on the fluxes of the first photons detected by the detectors, and the position of the attenuator over time, an imaging system may be configured to generate an image of the FOV.
Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.
Type:
Grant
Filed:
March 15, 2021
Date of Patent:
November 7, 2023
Assignee:
University of Washington
Inventors:
Jens Gundlach, Ian Michael Derrington, Andrew Laszlo, Jonathan Craig, Henry Brinkerhoff
Abstract: The invention provides methods for preparing DNA sequencing libraries by assembling short read sequencing data into longer contiguous sequences for genome assembly, full length cDNA sequencing, metagenomics, and the analysis of repetitive sequences of assembled genomes.
Type:
Application
Filed:
October 27, 2022
Publication date:
November 2, 2023
Applicant:
University of Washington
Inventors:
Jay Shendure, Joseph Hiatt, Rupali Patwardhan, Emily Turner
Abstract: Apparatuses, systems, and methods for dynamically shaped focal surface with a scanning microscope. A microscope (e.g., a scanning confocal microscope) may use a scanning element to scan an illumination beam to generate a focal region. The scanning element may include multiple degrees of freedom, such as rotation and translation along orthogonal axes. For example, if the illumination beam is a line focus, rotation along a first axis and translation along a second orthogonal axis may sweep the line focus across a focal surface which is substantially flat and normal to the second axis. In some embodiments, the microscope may be a dual-axis handheld microscope, where a single objective in a handheld housing is used to both direct the scanned illumination beam and receive the collected light.
Type:
Grant
Filed:
December 15, 2020
Date of Patent:
October 31, 2023
Assignee:
University of Washington
Inventors:
Jonathan T. C. Liu, Lingpeng Wei, Chengbo Yin
Abstract: Beta-sheet forming polypeptides at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to the amino acid sequence selected from the group consisting of SEQ ID NOS:1-24 are disclosed, together with their use and methods for designing beta-sheet forming polypeptides.
Type:
Grant
Filed:
October 23, 2020
Date of Patent:
October 31, 2023
Assignee:
UNIVERSITY OF WASHINGTON
Inventors:
Tamuka Chidyausiku, Enrique Marcos, Lucas Nivon, Gustav Oberdorfer, David Baker, Lauren Carter
Abstract: The present disclosure relates to bacterium engineered to produce aromatic compounds or compounds with aromatic metabolites or intermediates using the CRISPR-CAS transcriptional activation (CRISPRa) and/or transcriptional repression (CRISPRi). Accordingly, in an aspect the present disclosure relates to an engineered bacterium comprising genetic elements supporting programmable transcriptional activation and/or repression. The present disclosure also provides methods and systems for producing aromatic compounds or compounds with aromatic metabolites or intermediates using the engineered bacterium disclosed herein.
Type:
Application
Filed:
April 25, 2023
Publication date:
October 26, 2023
Applicant:
University of Washington
Inventors:
Cholpisit Kiattisewee, James M. Carothers, Jesse Zalatan, Ian D. Faulkner, Jason Fontana, Chen Dong