Patents Assigned to Thermo Fisher Scientific Baltics, UAB
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Patent number: 10308978Abstract: Transposon nucleic acids comprising a transposon end sequence and a calibration sequence for DNA sequencing in the transposon end sequence. In one embodiment, the transposon end sequence is a Mu transposon end. A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with the calibration sequence providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates.Type: GrantFiled: October 24, 2017Date of Patent: June 4, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Ian Kavanagh, Laura-Leena Kiiskinen, Heli Haakana
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Patent number: 10287614Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: May 2, 2017Date of Patent: May 14, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: 10273530Abstract: This disclosure relates to antibodies that bind thermophilic family B DNA polymerases. Related uses, methods, and compositions are also provided.Type: GrantFiled: January 13, 2017Date of Patent: April 30, 2019Assignee: Thermo Fisher Scientific Baltics UABInventors: Renata Rimseliene, Donata Ulcinaite, Birute Gagiliene, Rasa Sukackaite, Aurelija Zvirbliene, Indre Kucinskaite-Kodze, Dovile Dekaminaviciute
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Patent number: 10196666Abstract: A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.Type: GrantFiled: July 1, 2016Date of Patent: February 5, 2019Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Juozas {hacek over (S)}iurkus, Peter Neubauer
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Publication number: 20190010482Abstract: Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic DNA polymerase lacking 5?-3? and 3?-5? exonuclease activity premixed with T4 DNA polymerase, Klenow fragment and T4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform DNA blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps. Among other benefits, the mixture of different enzymes, buffers and nucleoside triphosphates is stable during prolonged storage.Type: ApplicationFiled: June 19, 2018Publication date: January 10, 2019Applicant: Thermo Fisher Scientific Baltics UABInventors: Judita Lubiene, Arturas Berezniakovas, Arvydas Lubys
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Publication number: 20180355428Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.Type: ApplicationFiled: May 25, 2018Publication date: December 13, 2018Applicant: Thermo Fisher Scientific Baltics UABInventors: Jukka Tuunanen, Jaako Kurkela, Katja Eklin
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Publication number: 20180298414Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: April 20, 2018Publication date: October 18, 2018Applicant: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Patent number: 10023856Abstract: Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic DNA polymerase lacking 5?-3? and 3?-5? exonuclease activity premixed with T4 DNA polymerase, Klenow fragment and T4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform DNA blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps. Among other benefits, the mixture of different enzymes, buffers and nucleoside triphosphates is stable during prolonged storage.Type: GrantFiled: September 24, 2014Date of Patent: July 17, 2018Assignee: Thermo Fisher Scientific Baltics UABInventors: Judita Lubiene, Arturas Berezniakovas, Arvydas Lubys
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Patent number: 10006086Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.Type: GrantFiled: July 14, 2016Date of Patent: June 26, 2018Assignee: Thermo Fisher Scientific Baltics UABInventors: Jukka Tuunanen, Jaako Kurkela, Katja Eklin
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Patent number: 9982268Abstract: A vector for transformation into a host cell is described comprising a toxic gene encoding a product that is lethal to the host cell, wherein the toxic gene comprises: an essential sequence region whose integrity is necessary in order for the encoded toxic gene product to be lethal to the host cell; an inessential sequence region whose integrity is not essential in order for the encoded toxic gene product to be lethal to the host cell; a regulatory sequence inserted in-frame into the inessential sequence region; and a cloning site within the essential sequence region for insertion of a nucleic acid sequence, wherein the regulatory sequence and the cloning site are positioned so as to allow the regulatory sequence to be operably linked to a nucleic acid sequence when the nucleic acid sequence is inserted into the cloning site.Type: GrantFiled: July 3, 2008Date of Patent: May 29, 2018Assignee: Thermo Fisher Scientific Baltics UABInventor: Arvydas Lubys
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Patent number: 9885025Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: GrantFiled: July 14, 2016Date of Patent: February 6, 2018Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Tadas Povilaitis
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Patent number: 9885074Abstract: A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with an engineered cleaveage site providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates. Transposon nucleic acids comprising a transposon end sequence and an engineered cleaveage site located in the sequence, e.g., in Mu transposon end sequence, are disclosed.Type: GrantFiled: September 8, 2014Date of Patent: February 6, 2018Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Ian Kavanagh, Laura-Leena Kiiskinen, Heli T. Haakana
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Patent number: 9856456Abstract: A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.Type: GrantFiled: September 24, 2015Date of Patent: January 2, 2018Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20170298403Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain n o more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: May 2, 2017Publication date: October 19, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20170267982Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: ApplicationFiled: February 1, 2017Publication date: September 21, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Publication number: 20170204454Abstract: This disclosure relates to antibodies that bind thermophilic family B DNA polymerases. Related uses, methods, and compositions are also provided.Type: ApplicationFiled: January 13, 2017Publication date: July 20, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Renata Rimseliene, Donata Ulcinaite, Birute Gagiliene, Rasa Sukackaite, Aurelija Zvirbliene, Indre Kucinskaite-Kodze, Dovile Dekaminaviciute
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Publication number: 20170204384Abstract: This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3?-5? exonuclease domain and/or a sequence non-specific dsDNA binding domain.Type: ApplicationFiled: January 13, 2017Publication date: July 20, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Renata Rimseliene, Rasa Sukackaite, Martyna Simutyte, Skaiste Valinskyte, Karolis Matjosaitis, Mindaugas Ukanis
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Patent number: 9683251Abstract: A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: June 19, 2013Date of Patent: June 20, 2017Assignee: Thermo Fisher Scientific Baltics UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20170152487Abstract: A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.Type: ApplicationFiled: September 24, 2015Publication date: June 1, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Patent number: 9650675Abstract: A restriction endonuclease with a recognition sequence 5?-TCGA-3?. The restriction endonuclease is sensitive to the presence of a modified cytosine residue in the recognition sequence. Methods and kits using the restriction endonuclease with a recognition sequence 5?-TCGA-3? are also disclosed.Type: GrantFiled: December 17, 2015Date of Patent: May 16, 2017Assignee: Thermo Fisher Scientific Baltics UABInventors: Renata Rimseliene, Remigijus Skirgalia, Arvydas Lubys