Patents Assigned to Thermo Fisher Scientific Baltics, UAB
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Patent number: 9617588Abstract: Polymorphisms are present throughout an organism's genome, and understanding which alleles are present in a particular organism's genome can be advantageous. When probing the identity of these alleles, one must minimize incorrect readings due to inefficiencies in the system. In hydrolysis probe applications, these inefficiencies may be due to over-activity of an exonuclease functionality that excises nucleotides from probes that are only partially, complementary to a region of a target. The present invention provides a mixture that contains a plurality of polymerases including one that has a 5??3? exonuclease functionality and one that lacks or substantially lacks it, each in a sufficient relative amount and concentration to increase efficiencies of the system.Type: GrantFiled: October 4, 2011Date of Patent: April 11, 2017Assignee: Thermo Fisher Scientific Baltics UABInventor: Jaakko Kurkela
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Publication number: 20170081646Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.Type: ApplicationFiled: September 29, 2016Publication date: March 23, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Agne Tubeleviciute, Renata Rimseliene, Sigitas Burinskas
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Patent number: 9598679Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: GrantFiled: September 9, 2010Date of Patent: March 21, 2017Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Publication number: 20170029861Abstract: A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.Type: ApplicationFiled: July 1, 2016Publication date: February 2, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Juozas Siurkus, Peter Neubauer
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Publication number: 20170015980Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: ApplicationFiled: July 14, 2016Publication date: January 19, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Tadas Povilaitis
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Publication number: 20170009291Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.Type: ApplicationFiled: July 14, 2016Publication date: January 12, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Jukka Tuunanen, Jaako Kurkela, Katja Eklin
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Patent number: 9493848Abstract: The invention provides novel mutant thermostable DNA polymerases having desirable properties such as increased polymerase efficiency and speed, increased affinity to DNA substrates and/or increased resistance to DNA polymerase inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel DNA polymerases.Type: GrantFiled: August 2, 2012Date of Patent: November 15, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Agne Tubelevi{hacek over (c)}iūté, Renata Rim{hacek over (s)}eliėnė, Sigitas Burinskas
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Patent number: 9481862Abstract: A composition and method for delivering a peptide or protein into a cell comprising a polycationic agent and a polyanionic agent, wherein the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate and/or the polycationic agent comprises a polyalkyleneimine.Type: GrantFiled: January 3, 2014Date of Patent: November 1, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20160312268Abstract: The invention relates to a method of preparing a reaction mixture for Polymerase Chain Reaction (PCR) assay and a solution set for PCR. The method comprises providing a sample solution comprising a biological sample to be amplified in said PCR assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the PCR process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors. The invention significantly aids in pipetting PCR assays.Type: ApplicationFiled: April 27, 2016Publication date: October 27, 2016Applicant: Thermo Fisher Scientific Baltics UABInventors: Jaakko KURKELA, Katja EKLIN, Sanna UUSIVIRTA
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Patent number: 9422604Abstract: The invention relates to a sample processing apparatus comprising a holder for a microtiter plate comprising a plurality of microwells, optical measurement unit for measuring optical responses of samples dosed to the microwells, and a computing unit configured to analyze the optical responses in order to detect dosing failures in said plurality of microwells, and if a dosing failure has been detected in one or more of the microwells, to communicate the existence of the dosing failure to a user of the apparatus through signaling means or to store data indicative of the dosing failure to data storage means for further use. In particular, the invention relates to detecting dosing failures in before, during and after a PCR process.Type: GrantFiled: April 1, 2011Date of Patent: August 23, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Jukka Tuunanen, Jaakko Kurkela, Katja Eklin
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Patent number: 9422535Abstract: Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template.Type: GrantFiled: December 20, 2013Date of Patent: August 23, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Remigijus Skirgaila, Tadas Povilaitis
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Patent number: 9416417Abstract: The invention relates to a method of preparing a reaction mixture for Polymerase Chain Reaction (PCR) assay and a solution set for PCR. The method comprises providing a sample solution comprising a biological sample to be amplified in said PCR assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the PCR process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors. The invention significantly aids in pipetting PCR assays.Type: GrantFiled: March 3, 2014Date of Patent: August 16, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Jaakko Kurkela, Katja Eklin, Sanna Uusivirta
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Patent number: 9410177Abstract: A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.Type: GrantFiled: January 20, 2012Date of Patent: August 9, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Juozas {hacek over (S)}iurkus, Peter Neubauer
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Patent number: 9273304Abstract: The present invention relates to the field of thermostable enzymes, methods for their inactivation, and related uses, kits and reagents. In particular, the invention relates to a method for reversible inactivation of thermophilic enzymes by chemical modification under aqueous or non-aqueous conditions.Type: GrantFiled: January 5, 2007Date of Patent: March 1, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Daiva Tauraite, Jurgita Barkauskaite, Leonas Grinius
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Publication number: 20160024475Abstract: A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.Type: ApplicationFiled: September 24, 2015Publication date: January 28, 2016Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Patent number: 9102796Abstract: The present invention relates to a polyalkyleneimine polymer having a repeat unit comprising a hydrophilic group, agents containing the same and methods for their use.Type: GrantFiled: December 12, 2008Date of Patent: August 11, 2015Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Sarunas Zigmantas, Laurynas Riauba, Lolita Zaliauskiene, Richard Makuska, Ausvydas Vareikis, Ula Bernadisiute
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Patent number: 9079157Abstract: The present invention provides compositions, methods and kits for the removal of proteins from complex reaction mixtures useful in majority workflows of molecular biology research experiments. More specifically, such compositions, methods and kits are useful in such processes as purification of nucleic acids from biological samples or after their treatment with specific enzymes, when residual enzyme activity in reaction mixture is not compatible with downstream applications.Type: GrantFiled: July 29, 2013Date of Patent: July 14, 2015Assignee: Thermo Fisher Scientific Baltics, UABInventors: Vitalis Tiknius, Eglé Merkiene
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Patent number: 8835148Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: GrantFiled: October 7, 2010Date of Patent: September 16, 2014Assignee: Thermo Fisher Scientific Baltics, UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20140178880Abstract: The invention relates to a method of preparing a reaction mixture for Polymerase Chain Reaction (PCR) assay and a solution set for PCR. The method comprises providing a sample solution comprising a biological sample to be amplified in said PCR assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the PCR process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors. The invention significantly aids in pipetting PCR assays.Type: ApplicationFiled: March 3, 2014Publication date: June 26, 2014Applicant: Thermo Fisher Scientific Baltics UABInventors: Jaakko KURKELA, Katja Eklin, Sanna Uusivirta
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Publication number: 20140120618Abstract: A composition and method for delivering a peptide or protein into a cell.Type: ApplicationFiled: January 3, 2014Publication date: May 1, 2014Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene