Abstract: The present disclosure provides a one-pot chemoenzymatic method for site-specific modification and conjugation of antibodies at their Fc glycan site to produce structurally well-defined antibody conjugates carrying defined drugs and other entities. The method is enabled by the discovery that certain endoglycosidases have the ability to both deglycosylate an antibody and to recognize selectively modified small disaccharide oxazolines for transglycosylation on antibodies without hydrolysis of the resulting products.

Abstract: Examples of the present disclosure generally relate to systems, methods, and devices for performing electrochemical control and monitoring of bacterial gene expression to a precisely assigned level, and may be used, for example, for controlling the production of a protein of interest.

Abstract: A microfluidic device including at least one channel in fluid communication with a sample trap array. Specifically, the configuration and geometry of the trap arrays according to the present invention allows for performing sample digitization that supports passive self-discretization within the sample traps without the need for any external flow control or actuation. Geometrical parameters defining the sample traps, including the trap width and the trap depth, are selected to optimize self-filling of the sample traps. Reagents are incorporated into the sample traps during device fabrication to allow for performing multiplexed reactions within the sample traps.

Abstract: A device and method for detecting the presence of bacteria in a sample are provided. A multi-step process for sample preparation is utilized and a microfluidic device is disclosed. The detection is performed using microfluidics and physical changes in multiple samples in differential mode.

Abstract: Bare porous polymer monoliths, fluidic chips, methods of incorporating bare porous polymer monoliths into fluidic chips, and methods for functionalizing bare porous polymer monoliths are described. Bare porous polymer monoliths may be fabricated ex situ in a mold. The bare porous polymer monoliths may also be functionalized ex situ. Incorporating the bare preformed porous polymer monoliths into the fluidic chips may include inserting the monoliths into channels of channel substrates of the fluidic chips. Incorporating the bare preformed porous polymer monoliths into the fluidic chips may include bonding a capping layer to the channel substrate. The bare porous polymer monoliths may be mechanically anchored to channel walls and to the capping layer. The bare porous polymer monoliths may be functionalized by ex situ immobilization of capture probes on the monoliths. The monoliths may be functionalized by direct attachment of chitosan.

Abstract: Solid-state lithium ion electrolytes of lithium phosphate derivative compounds are provided which contain an anionic framework capable of conducting lithium ions. The activation energy of the lithium phosphate derivative compounds is from 0.18 to 0.34 eV and conductivities are from 10?3 to 12 mS/cm at 300K. Materials of specific formulae are provided and methods to alter the composite materials with inclusion of aliovalent ions shown. Lithium batteries containing the composite lithium ion electrolytes are also provided. Electrodes containing the lithium phosphate derivative materials and batteries with such electrodes are also provided.

Abstract: The present invention relates an indicator system for assessing a reduction state of unconsolidated material that includes a delivery tube defining an interior chamber, and a substrate disposed within the interior chamber and including a reactive coating thereon. The reactive coating is at least partially removable from the substrate upon exposure to a reducing condition of unconsolidated material over a period of time. An indicator device including a reactive coating comprising a manganese oxide is also disclosed.

Abstract: Compositions and methods for improved delivery of mRNA into eukaryotic cells using histidine-lysine (HK) peptide polymers are disclosed. The branched polymers comprising four short peptide branches linked to a three-lysine amino acid core. The peptide branches consist of histidine and lysine amino acids, in different configurations, and they can vary in their location on the lysine core.

Abstract: The present invention relates to a microfluidic system including a temperature controller and a thermoplastic microfluidic chip that enables rapid PCR in a PCR chamber of the microfluidic chip. Thermal control of the PCR chamber is achieved by applying voltage to heater electrodes patterned directly onto one layer of the microfluidic chip. The temperature controller adjusts the voltage applied to the heater electrodes by changing temperature controller parameters selected to minimize duration of each PCR cycle. Furthermore, simple operation of the microfluidic chip is provided through using an integrated passive capillary valve, requiring minimum operator intervention and eliminating the need for fluidic interfacing, pumping, or metering during chip loading.

Abstract: An implantable acellular polymeric scaffold device functionalized with aggrecan is provided. Also provided are methods of fabricating a polymeric scaffold device, including methods of fabricating the scaffold device via 3D printing. Methods of treating a cartilage defect in a subject in need thereof comprise application of the disclosed scaffold device in combination with microfracture procedures. A specialized lid for a centrifugation well plate is also provided.

Abstract: The present invention relates in part to compositions for targeted delivery of an agent and agent delivery systems comprising a novel tissue-targeting peptide ligand (CKPFDRALC) (SEQ ID NO: 1) named ART-2. In certain aspects, the ART-2-coated liposomes encapsulating an agent, such as a therapeutic agent, diagnostic agent, imaging agent, or any combination thereof, were more effective in inhibiting, diagnosing, or imaging a disease or disorder, such as an arthritis progression, than control, despite a comparable safety profile.

Abstract: Systems, methods, and media for wireless power transfer are provided.

Abstract: Mcl-1 selective inhibitors, Bcl-2 selective inhibitors, and Mcl-1/Bcl-2 dual inhibitors and methods of using the same for the treatment of disease are disclosed.

Abstract: The present invention provides broadly neutralizing antibodies against HIV, compositions comprising the same and methods of use thereof.

Abstract: Phospholipid-conjugated Ags were used as an agnostic delivery platform to cell type, activation state, and inherent uptake capabilities for engineering APCs to control Ag-specific cellular immune responses. Lipid-mediated delivery (termed depoting) of MHC class I and II-restricted Ags successfully loaded resting polyclonal B cells, CD40? activated B cells, and DCs in a dose-dependent manner for priming Ag-specific CD8+ and CD4+ T cells, respectively. When lipid-conjugated Ags were paired with polymer-conjugated Ags and incorporated in nanoparticles (NPs), diverse APCs with varying NP internalization capabilities all processed the lipid-conjugated Ags via depoting while only DCs processed the PLGA-conjugated Ags via endocytosis. Multivariate analyses of cytokine secretions indicated that lipid-conjugated Ags could be distinctly classified from polymer-conjugated Ags.

Abstract: A method and apparatus is provided for automatic emergency airway detection. The apparatus includes a frame with a carriage guide configured to be secured around a neck of a subject. A carriage including a sensor is configured to move along the carriage guide. The sensor is configured to measure a value of a parameter indicating a topography of the neck of the subject. A processor includes a memory with instructions to receive first data from the sensor of the value of the parameter as the carriage is moved along the carriage guide and to determine, with the first data, second data indicating a position along the carriage guide corresponding to the cricothyroid region of the neck. The processor subsequently transmits a first signal indicating the second data. A method of using the apparatus is also provided.

Abstract: A robotic system for remote operation of human-controllable equipment includes a remotely operable robotic subsystem configured to be at least one of mounted on or arranged adjacent to the human-controllable equipment to be controlled, and a remote controller that is configured to be operated by a human. The remote controller emulates a control representation of the human-controllable equipment such that a human can provide commands using the control representation. The remotely operable robotic subsystem is configured to receive commands from the remote controller to thereby operate the human-controllable equipment.

Abstract: The present invention provides compositions and methods of inducing an immune response in a subject in need thereof, comprising administering to the subject an immunologically-effective amount of a live Salmonella Typhi vector comprising a heterologous antigen from a pathogen, wherein the heterologous antigen comprises an outer membrane protein, an antigenic fragment thereof or a variant thereof, wherein the antigen is delivered to a mucosal tissue of the subject by an outer membrane vesicle.

Abstract: An aortic aneurysm carries increasing risk of rupture with growing aneurysm diameter. This condition is typically asymptomatic, so screening and surveillance are essential. Ultrasound and other imaging methods are employed for such monitoring at high accuracy. However, these methods require an expert and are expensive. Aortic aneurysms are considerably under-detected at present and may become even more under-detected in the future as the disease prevalence increases with societal aging. This disclosure present devices that are convenient in use and cost for aortic aneurysm screening and surveillance.

Abstract: A new and distinctive variety of Malus domestica Borkh, apple tree named ‘MD-TAP2’ is distinguished by its attractive tree architecture, reduced need for hand pruning and training, observed tolerance to high temperatures during the growing season, fireblight (Erwinia amylovora) and fruit which is harvested two weeks earlier than its seed parent.