Patents Examined by Anne Gussow
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Patent number: 9695481Abstract: The invention provides a polynucleotide comprising a reporter sequence operatively linked to a regulatory element of a gene selected from Bscl2, Srxnl, Cbr3, Ephxl, Nope, Cdknla, Perp, Pltp, Cgrefl, Ltb4r1, Btg2, Gpx2, Ltb4r2, Ddit4l, Fosl1, and Egr1, which regulatory element stimulates expression of the reporter sequence in response to a genotoxic agent or to an oxidative stress-inducing agent. The invention also provides a method of detecting a genotoxic or oxidative stress-inducing agent comprising subjecting a cell containing the polynucleotide of the invention to a test agent; and assessing the expression of the reporter sequence.Type: GrantFiled: September 15, 2011Date of Patent: July 4, 2017Assignees: UNIVERSITEIT LEIDEN, ACADEMISCH ZIEKENHUIS LEIDENInventors: Bob Van de Water, Harm Vrieling
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Patent number: 9650630Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences from the promoter region of a 60S ribosomal protein L32 gene (RPL32), for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.Type: GrantFiled: August 31, 2011Date of Patent: May 16, 2017Assignee: CellaGenics B.V.Inventors: Arie Pieter Otte, Femke Hoeksema, John Antonius Verhees, Henricus Johannes Maria Van Blokland
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Patent number: 9605268Abstract: Disclosed is a method for introducing an exogenous DNA by overcoming the restriction modification barrier of the target bacterium. The method includes the steps of 1) co-expressing all DNA-methyltransferase-encoding genes in the genome of the target bacterium in E. coli in which the restriction modification system thereof has been deleted to obtain a recombinant bacterium A, 2) introducing an exogenous DNA molecule into the recombinant bacterium A for in vivo modification so as to obtain a methylation-modified exogenous DNA molecule, and 3) introducing the methylation-modified exogenous DNA molecule into the target bacterium.Type: GrantFiled: February 21, 2013Date of Patent: March 28, 2017Assignee: Institute of Microbiology, Chinese Academy of SciencesInventors: Tingyi Wen, Guoqiang Zhang, Aihua Deng
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Patent number: 9512431Abstract: Methods for making synthetic gene clusters are described.Type: GrantFiled: June 14, 2012Date of Patent: December 6, 2016Assignee: The Regents of the University of CaliforniaInventors: Ethan Mirsky, Karsten Temme, Christopher A. Voigt, Dehua Zhao
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Patent number: 9495515Abstract: The present invention relates to compositions and methods for molecular profiling and diagnostics for genetic disorders and cancer, including but not limited to gene expression product markers associated with cancer or genetic disorders. In particular, the present invention provides algorithms and methods of classifying cancer, for example, thyroid cancer, methods of determining molecular profiles, and methods of analyzing results to provide a diagnosis.Type: GrantFiled: December 9, 2010Date of Patent: November 15, 2016Assignee: Veracyte, Inc.Inventors: Giulia C. Kennedy, Darya I. Chudova, Eric T. Wang, Jonathan I. Wilde
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Patent number: 9486486Abstract: The present invention relates to a novel bacterial strain isolated and characterized from groundwater. The invention also relates to bacterial extracts and to the therapeutic use of same, notably in the context of the treatment of inflammations.Type: GrantFiled: December 22, 2011Date of Patent: November 8, 2016Assignees: PIERRE FABRE DERMO-COSMETIQUE, PIERRE FABRE MEDICAMENTInventors: Nathalie Castex-Rizzi, Thien Nguyen, Christine Libon, Bertrand Chol
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Patent number: 9481914Abstract: The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.Type: GrantFiled: October 30, 2015Date of Patent: November 1, 2016Assignee: Quest Diagnostics Investments IncorporatedInventors: Erik P. Johnson, Dale A. Schwab
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Patent number: 9434985Abstract: The disclosed Hi-C protocol can identify genomic loci that are spatially co-located in vivo. These spatial co-locations may include, but are not limited to, intrachromosomal interactions and/or interchromosomal interactions. Hi-C techniques may be applied to many different scales of interest. For example, on a large scale, Hi-C techniques can be used to identify long-range interactions between distant genomic loci.Type: GrantFiled: September 22, 2009Date of Patent: September 6, 2016Assignees: University of Massachusetts, Massachusetts Institute Of Technology, President And Fellows Of Harvard College, Whitehead Institute For Biomedical ResearchInventors: Job Dekker, Erez Lieberman, Nynke Van Berkum, Andreas Gnirke, Eric Lander, Chad Nusbaum, Louise Williams, Alexandre Melnikov, Georgia Giannoukos
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Patent number: 9410208Abstract: The present invention relates to a marker allowing specific detection of human IL-17-producing helper T-cells (human Th17 cells), a method for specifically detecting human Th17 cells and a reagent for detecting human Th17 cells.Type: GrantFiled: April 23, 2013Date of Patent: August 9, 2016Assignee: SYSMEX CORPORATIONInventors: Satoshi Tanaka, Hitoshi Uga, Masafumi Ikeda, Yoshiaki Miyamoto, Masatoshi Yanagida, Masakazu Kadowaki, Takahiro Okazawa, Hirokazu Kurata
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Patent number: 9404138Abstract: A novel technique for improving ability for secretory production of a multimeric protein of a coryneform bacterium is provided, and thereby a method for secretory production of a multimeric protein is provided. A multimeric protein is produced by secretory production utilizing a coryneform bacterium having an ability to produce a multimeric protein by secretory production and modified so that expression of a gene coding for a metallopeptidase is increased as an expression host.Type: GrantFiled: April 29, 2014Date of Patent: August 2, 2016Assignee: AJINOMOTO CO., INC.Inventors: Yoshihiko Matsuda, Haruki Beppu
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Patent number: 9366681Abstract: Disclosed are: a polypeptide marker for diagnosing arteriosclerosis; a gene marker for diagnosing arteriosclerosis; an antibody; a probe for detecting an arteriosclerosis marker gene; a DNA microarray or a DNA chip for detecting an arteriosclerosis marker gene; a method for detecting arteriosclerosis; and a kit for diagnosing arteriosclerosis; with which an arteriosclerotic lesion can be detected with much improved accuracy.Type: GrantFiled: March 4, 2013Date of Patent: June 14, 2016Assignees: Fujikura Kasei Co., Ltd., National University Corporation Chiba UniversityInventors: Rika Nakamura, Hideyuki Kuroda, Go Tomiyoshi, Takaki Hiwasa, Masaki Takiguchi, Naokatsu Saeki, Toshio Machida
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Patent number: 9347088Abstract: The present invention concerns a method to determine the gene expression profile on a sample previously obtained from a patient diagnosed for a liver tumor, comprising assaying the expression of a set of genes in this sample and determining the gene expression profile (signature). In a particular embodiment, said method enables to determine the grade of the liver tumor, such as hepatoblastoma (HB) or a hepatocellular carcinoma (HCC). The invention is also directed to kits comprising a plurality of pairs of primers or a plurality of probes specific for a set of genes, as well as to solid support or composition comprising a set of probes specific for a set of genes. These methods are useful to determine the grade of a liver tumor in a sample obtained from a patient, to determine the risk of developing metastasis and/or to define the therapeutic regimen to apply to a patient.Type: GrantFiled: June 26, 2009Date of Patent: May 24, 2016Assignees: Institut Pasteur, Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche ScientifiqueInventors: Marie-Annick Buendia, Carolina Armengol Niell, Stefano Cairo, Aurélien de Reynies
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Patent number: 9315857Abstract: Compositions, methods and kits are disclosed for high-sensitivity counting of individual molecules by stochastic labeling of a identical molecules in mixtures of molecules by attachment of a unique label-tags from a diverse pool of label tags to confer uniqueness to otherwise identical or indistinguishable events. Individual occurrences of target molecules randomly choose from a non-depleting reservoir of diverse label-tags. Labeled molecules may be detected by hybridization or sequencing based methods. Molecules that would otherwise be identical in information content are labeled to create a separately detectable product that can be distinctly detected. The disclosed stochastic transformation methods reduce the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed label-tags are present. The methods may be used, for example, to count a given species of molecule within a sample.Type: GrantFiled: December 15, 2011Date of Patent: April 19, 2016Assignee: CELLULAR RESEARCH, INC.Inventors: Glenn K. Fu, Stephen P. A. Fodor
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Patent number: 9315588Abstract: Provided is a novel hyaluronan synthase (HAS2), isolated nucleic acids encoding it, and expression vectors which express the novel HAS2. Also provided are cell cultures which contain cells which harbor the expression vectors, methods of using the cell cultures to produce high molecular weight hyaluronic acid, and cell culture media that contains the high molecular weight hyaluronic acid.Type: GrantFiled: February 4, 2013Date of Patent: April 19, 2016Assignee: University of RochesterInventors: Vera Gorbunova, Andrei Seluanov
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Patent number: 9309503Abstract: The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.Type: GrantFiled: July 4, 2008Date of Patent: April 12, 2016Assignee: DPx Holdings B.V.Inventors: Martin Kietzmann, Helmut Schwab, Harald Pichler, Mirela Ivancic, Oliver May, Rudolf Gijsbertus Marie Luiten
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Patent number: 9301993Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of oncology-related polynucleotides, oncology-related primary transcripts and oncology-related mmRNA molecules.Type: GrantFiled: December 16, 2013Date of Patent: April 5, 2016Assignee: Moderna Therapeutics, Inc.Inventors: Tirtha Chakraborty, Antonin de Fougerolles
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Patent number: 9297024Abstract: The invention provides expression vectors and host cells for high-level expression of recombinant proteins. The expression vectors comprise Chinese hamster ovary elongation factor 1-? (CHEF1) transcriptional regulatory DNA elements and a cytomegalovirus (CMV) promoter and/or a human adenovirus tripartite leader (AdTPL) sequence. The invention achieves increased protein expression and better productivity of host cells compared to previously described expression systems.Type: GrantFiled: March 11, 2014Date of Patent: March 29, 2016Assignee: CMC ICOS BIOLOGICS, INC.Inventor: Howard R. Clarke
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Patent number: 9290809Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.Type: GrantFiled: November 12, 2014Date of Patent: March 22, 2016Assignee: Cellular Research, Inc.Inventors: Stephen P. A. Fodor, Glenn K. Fu
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Patent number: 9290808Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.Type: GrantFiled: November 12, 2014Date of Patent: March 22, 2016Assignee: Cellular Research, Inc.Inventors: Stephen P. A. Fodor, Glenn K. Fu
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Patent number: 9284582Abstract: The present invention relates to a method for preparing a mutant E. coli strain, capable of simultaneously using glucose and xylose, by genetic engineering and evolutionary adaptation; the mutant E. coli prepared using the same; and a method for producing biofuels, biologically active ingredients, medicinal materials or base chemicals for the chemical industry using the mutant E. coli. Being capable of simultaneously using glucose and xylose, in contrast to wild-type E. coli, the mutant E. coli can be effectively applied to the enzymatic saccharification process of producing biofuels from a biomass.Type: GrantFiled: July 2, 2014Date of Patent: March 15, 2016Assignee: UNIST Academy-Industry Research CorporationInventors: Sung Kuk Lee, Goo Hee Kim, Seong Hun Jeong, Suk Min Kim, Bae Young Choi