Abstract: The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.

Abstract: Ion exchange resin macroporous beads for the highly selective extraction of univalent anions from aqueous solutions. A specific example is the removal of dicyanoaurate and dicyanoargentate from cyanide leach solutions and tailings. The beads have a maximum number of ligands specific for the desired univalent anion, while maintaining sufficient separation to minimize binding of polyvalent ions. The beads are prepared using a functionalized monomer with the use of a specifically tuned coordinator. The beads can be used as a sensor for detecting the amount of anions captured when interrogated by an appropriate light source.

Abstract: An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. The illumination may be received from a top, a side edge, and a bottom of the device. The waveguide assembly may include a nanoscale aperture disposed in the substrate and extending through the cladding. The aperture defines a reaction cell for receiving a set of reactants. In various aspects, the device includes a sensor element and the illumination pathway is through the sensor element. Waveguides and illumination devices, such as plasmonic illumination devices, are also disclosed. Methods for forming and operating the devices are also disclosed.

Abstract: Disclosed is a chip.

Abstract: An analysis system and a method for testing, in particular, a biological sample, the sample and/or analytes of the sample being temperature-controlled in advance or denatured immediately before the test, and/or being heated, the heat being transferred through a chip of a sensor apparatus.

Abstract: Disclosed are a gene detection device including gold nanoparticles and a gene detection method using gold nanoparticles. The use of the gene detection device and the gene detection method avoids the need for special equipment, such as a thermal cycler, which is essential for 3-stage heating in conventional PCR-based gene amplification techniques. In addition, the gene detection device and the gene detection method enable rapid and sensitive quantitative analysis and multiple detection.

Abstract: A molecular manipulation system for investigating molecules, having a sample holder constructed to hold a sample comprising a plurality of molecules attached on one side to a surface in the sample holder and on another side attached to a microbead of a plurality of microbeads. The system having; an acoustic wave generator to generate an acoustic wave exerting a force on the microbeads in the sample; and a detector device to detect a response of the plurality of microbeads in the sample on the force exerted by the acoustic wave to investigate the molecules attached to the microbeads.

Abstract: Sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising two electrodes separated by an insulating layer are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the insulating region. A sequencing reaction mixture comprising nucleotide analogs having impedance labels is introduced to the array of nanoscale electronic elements under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance.

Abstract: The invention proposes a device for analyzing nucleic acid molecules (M), comprising: —a bead (20), on which one molecule can be anchored at one end, —a surface (520), on which the molecule can be anchored at the other end, —an actuator (30), adapted to cause the bead to move relative to said surface in one direction, —a sensor (50), adapted to measure a distance between the bead and the surface, the device further comprising a well (11), having an axis (X-X) extending along the direction of motion of the bead and a bottom (110) formed by said surface, said well being filled with electrically conductive solution (40), and receiving the bead, the sensor being adapted to measure an impedance of the well, depending on a distance between the bead and the surface, to determine, the distance between the bead and the surface.

Abstract: In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.

Abstract: A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.

Abstract: In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and an enzyme molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the enzyme. In various examples, the enzyme is a polymerase. The circuit may further comprise molecular arms used to wire the enzyme to the electrodes. In various embodiments, the circuit functions as a sensor, wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as substrates interact with the enzyme.

Abstract: Presented are methods and compositions for spatial detection and analysis of nucleic acids in a tissue sample. The methods can enable the characterization of transcriptomes and/or genomic variations in tissues while preserving spatial information about the tissue.

Abstract: An array device including a base having wells, a cover positioned over the base with a gap from the base such that openings of the wells are covered by the cover with the gap in between, an injection port communicating with the gap, a discharge port communicating with the gap and positioned apart from the injection port, and a waste liquid vessel which collects liquid that flows from the gap via the discharge port and is positioned at a level different from the gap forming a channel. The discharge port is placed to discharge a surplus aqueous solution outside the wells from the discharge port by an oleaginous sealing liquid to be delivered from the injection port.

Abstract: The present invention discloses a multi-flux microfluidic chip for nucleic acid detection and capable of actively controlling a flow path, and a use method thereof. The detection chip includes a chip body, and the chip body is provided with a sample loading chamber, a reaction chamber, and a microfluidic channel, where there is a plurality of reaction chambers, and the microfluidic channel includes a sample output main channel and several sample distribution channels.

Abstract: Biosensors and methods for localized surface plasmon resonance biosensing are disclosed. The biosensor can include a substrate having a substrate surface to which a plurality of localized surface plasmon resonance (LSPR) antennae are affixed. The LSPR antennae can be affixed via an affixation surface of the LSPR antenna. The LSPR antennae can have a functional surface opposite the affixation surface. Each functional surface can be functionalized by a plurality of single-stranded DNA.

Abstract: A fluidic card assembly comprising a fluidic card housing (1) and a biochip (3) located in the fluidic card housing. The fluidic card housing (1) includes a chamber (2) with a base wall, into which at least one fluidic channel extends. The biochip (3) is at least partially located in the chamber. A seal (7) is provided for sealing the biochip in the chamber (2) when the biochip is urged into the chamber. The fluidic channel has a serpentine form.

Abstract: Methods, systems, and devices are disclosed for enrichment and detection of molecules of a target biomarker. In one aspect, In one aspect, a biosensor device for enriching and detecting biomarker molecules include a substrate, and a microarray of hydrophilic islands disposed on the substrate. A sensing area on each of the microarray hydrophilic islands is structured to anchor bio-molecular probes of at least one type for detecting molecules of a target biomarker and to attract an array of nanodroplets of a biomarker solution that includes the target biomarker molecules. A hydrophobic surface is disposed to surround the microarray of hydrophilic islands.

Abstract: Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal.

Abstract: The purpose of the invention is to provide a nucleic acid analyzer that sets and executes temperature regulation, said temperature regulation matching the characteristics of analyzing items and the configuration of the analyzer, by a simple operation while preventing deterioration in analytical performance caused by partial overheating of a liquid reaction mixture to thereby improve temperature change speed and shorten analysis time. To achieve the above purpose, provided is a method wherein, in the case of performing overshooting: as a first processing, the temperature is continuously elevated until reaching a target overshoot temperature; as a second processing, after reaching the aforesaid temperature, the overshoot target temperature is maintained for a preset period of time; and, as a third processing, the temperature is continuously lowered until reaching a target temperature of the liquid reaction mixture.