Patents Examined by Bin Shen
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Patent number: 11453858Abstract: In some aspects, described herein are cell culture media that are useful for in vitro culture of mammalian cells. The culture media contain a variety of small organic compounds that are found in normal adult human blood. Also described are methods of using the culture media for a variety of purposes. Also described are methods of treating cancer.Type: GrantFiled: November 13, 2017Date of Patent: September 27, 2022Assignee: Whitehead Institute for Biomedical ResearchInventors: David M. Sabatini, Jason Cantor
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Patent number: 11448645Abstract: Cultivation and/or test-system comprising at least two cultivation spaces combined in a microparticle, wherein the sperically shaped microparticle comprises, (i) a first cultivation space in the center core of said spherically shaped microparticle, (ii) a second cultivation space in the wall surrounding the core of said microparticle, (iii) wherein the wall surrounding the core allows for the exchange of molecules as, salts, nutrients, peptides, chemicals and other compounds in order for the cells in the first cultivation space to interact with the cells in second cultivation space and vice versa. In the test system, the cells are co-cultivated and the microparticles are then selected based on the phenotype of the cells in the first or second cultivation space.Type: GrantFiled: March 2, 2018Date of Patent: September 20, 2022Assignee: Biomillenia SASInventors: Dirk Loeffert, Aleksander Dajkovic, Thomas Duboys
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Patent number: 11447638Abstract: The present invention provides di-substituted acetalanthraquinone-based compounds of the Formulae (l)-(IV) that are useful for cellular staining and for light-based detection of biological material, e.g. fluorescence-based detection of biological material. These compounds may be used in hydrolysed or non-hydrolysed form. Also provided is a fluorescent complex that comprises a nucleic acid and an acetalanthraquinone-based compound of the invention; and a method of staining a biological sample comprising cells or other biological material containing nucleic acid, which method comprises contacting the biological sample with an acetalanthraquinone-based compound of the invention. In the Formulae (l)-(IV), A and B are each independently of formula —RaCH2CH(ORb)2; or one of A and B is of formula —RaCH2CH(ORb)2 and the other one is of formula: —NRb2.Type: GrantFiled: June 9, 2017Date of Patent: September 20, 2022Assignee: BIOSTATUS LIMITEDInventors: Paul James Smith, Rachel Jane Errington, Laurence Hylton Patterson, Klaus Pors, Laura Cosentino
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Patent number: 11439691Abstract: The present invention relates to an aqueous solution comprising burlulipase which is characterized in that it contains calcium ions. In particular, it also relates to corresponding pharmaceuticals. Such pharmaceuticals are suitable for the treatment of exocrine pancreatic insufficiency. These pharmaceuticals are particularly suitable for treating patients having cystic fibrosis and in pediatrics. Another aspect of the present invention relates to container-packaged solutions and pharmaceuticals as mentioned above, which are containers produced by the blow-fill-seal method, and to methods for producing such packaged solutions and pharmaceuticals.Type: GrantFiled: March 5, 2018Date of Patent: September 13, 2022Assignee: NORDMARK PHARMA GMBHInventors: Richard Fuhrherr, Annika Müller-Lucks, Katharina Rützel, Jan Lüdemann, Martin Werner
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Patent number: 11442063Abstract: Provided herein are compounds that are broad-spectrum protein kinase binding agents, detectable tracers comprising such compounds, and method of use thereof for the detection of protein kinases.Type: GrantFiled: May 30, 2019Date of Patent: September 13, 2022Assignee: Promega CorporationInventors: Cesear Corona, Poncho Meisenheimer, Matthew Robers, James Vasta
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Patent number: 11442072Abstract: The present disclosure relates to a method for measuring aggregation of low-density lipoprotein (LDL). The susceptibility of LDL to aggregate is an important factor regarding prognosis, diagnosis and surveillance of atherosclerotic cardiovascular diseases and cardiometabolic diseases, such as type 2 diabetes and related conditions, at various stages of their development. Also provided are methods of treatment of such diseases in subjects in need thereof.Type: GrantFiled: July 1, 2019Date of Patent: September 13, 2022Assignee: Jenny and Antti Wihuri FoundationInventors: Katariina Öörni, Maija Ruuth, Petri Kovanen
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Patent number: 11419830Abstract: The present invention is directed, in part, to methods of treating Epidermolysis Bullosa (EB) in a subject in need thereof, comprising topical administration of a pharmaceutical composition comprising a therapeutically effective amount of a of Coenzyme Q10 (CoQ10) to the subject.Type: GrantFiled: May 16, 2018Date of Patent: August 23, 2022Assignee: Berg LLCInventors: Niven Rajin Narain, Rangaprasad Sarangarajan, Michael Andrew Kiebish
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Patent number: 11414691Abstract: Provided are an organoid having a metastatic property when transplanted into an immunocompetent non-human animal of the same species, a cell strain having a metastatic property when transplanted into an immunocompetent non-human animal of the same species, and a non-human animal including the organoid or the cell strain.Type: GrantFiled: September 7, 2017Date of Patent: August 16, 2022Assignee: National University Corporation Kanazawa UniversityInventors: Masanobu Oshima, Mizuho Nakayama, Eri Sakai
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Patent number: 11401502Abstract: An object of the present invention is to provide highly proliferative mesenchymal stem cells (MSCs) useful for the large-scale and rapid production of a cell preparation, and a cell population comprising the mesenchymal stem cells. According to the present invention, there is provided a cell population comprising mesenchymal stem cells derived from the fetal appendage, wherein, in the cell population, the proportion of CD105+ mesenchymal stem cells is 50% or more, the proportion of CD200+ mesenchymal stem cells is less than 10%, and the proportion of CD106+ mesenchymal stem cells is less than 5%.Type: GrantFiled: November 14, 2017Date of Patent: August 2, 2022Assignee: KANEKA CORPORATIONInventors: Masahiro Hayashi, Yuta Kita, Nobuyoshi Umeda
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Patent number: 11401501Abstract: The present invention provides a method for culturing organoids, the method comprising: a) disassociating unprocessed organoids to produce a cell suspension; b) sieving the cell suspension through a cell strainer to retain a sieved cell suspension containing cells of about 10 ?m to about 1 mm in diameter; and c) seeding cells of the sieved cell suspension into a bioreactor in a cell culture medium comprising an extracellular support matrix.Type: GrantFiled: July 11, 2017Date of Patent: August 2, 2022Assignee: CELLESCE LIMITEDInventors: Marianne J. Ellis, Julian Chaudhuri, Trevor Clive Dale
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Patent number: 11396642Abstract: Provided are a tumour immunogen, a preparation method therefore, and an application. After localised sequential cooling-heating treatment is performed on tumour tissue and/or cells, the tumour tissue and/or cells release a large amount of tumour immunogen heat shock protein 70. The obtained tumour immunogen can activate the body's tumour immune system, to convert immunosuppressive cells into mature dendritic cells, thereby increasing immunogen presentation, and activating tumour immunity.Type: GrantFiled: October 11, 2017Date of Patent: July 26, 2022Assignee: MAGI COMPANY LTDInventors: Xuemin Xu, Ping Liu, Aili Zhang, Jingfeng Bai, Jianqi Sun, Jincheng Zou
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Patent number: 11384334Abstract: Provided are: a polymer gel for a medium, and a medium in which the stiffness of the polymer gel can be easily and reversibly changed and the shape of cells can be controlled according to the stiffness of the gel; and a method for culturing cells using the medium. The polymer gel for a medium contains a solvent and a crosslinked structure that is crosslinked by reversible bonds. The stiffness of the polymer gel for a medium can be easily and reversibly changed. Accordingly, when the polymer gel for a medium according to the present invention is used, cell morphology and function can be reversibly controlled according to the stiffness of the gel.Type: GrantFiled: July 25, 2017Date of Patent: July 12, 2022Assignees: Osaka University, Kyoto UniversityInventors: Akira Harada, Yoshinori Takashima, Masaki Nakahata, Motomu Tanaka, Marcel Hoerning
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Patent number: 11376593Abstract: Systems and methods for sorting T cells are disclosed. Autofluorescence data is acquired from individual cells. An activation value is computed using one or more autofluorescence endpoints as an input. The one or more autofluorescence endpoints includes NAD(P)H shortest fluorescence lifetime amplitude component (?1).Type: GrantFiled: August 28, 2019Date of Patent: July 5, 2022Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Melissa Caroline Skala, Alexandra Jule Walsh
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Patent number: 11378570Abstract: A cytotoxicity method for medical devices that has equivalent sensitivity, and greater efficiency compared to the colony formation assay (CFA) direct contact method is developed. The quantitative, direct contact cytotoxicity test method uses Mouse lymphoma TK or human lymphoblastoid TK6 suspension cells for medical device safety assessment. The advantages of using the suspension cell assay over adherent V79-4 cells is that this approach is quantitative, automated, not susceptible to potential mechanical damage imparted by the overlying test materials, and avoids issues related to non-adherence of cells to the test material which might impact the interpretation of test results.Type: GrantFiled: May 24, 2018Date of Patent: July 5, 2022Assignee: Alcon Inc.Inventors: Xuemei Liu, Fan Zhang, Denise P. Rodeheaver, Ann Marie Wright, Charlon Tolliver, Jamie Michaelis Walker, Robert Edward Rose, Stephen Paul Shannon, Jeffrey Charles White
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Patent number: 11377634Abstract: Disclosed herein is an integrated assay system that can be used, for example, to monitor and screen cells in 3D culture. This system involves a 3D cell growth medium made from a yield stress material that allows cells to be deposited, e.g. by 3D printing, samples to be taken, and the extracellular environment manipulated.Type: GrantFiled: November 7, 2017Date of Patent: July 5, 2022Assignee: University of Florida Research Foundation, Inc.Inventors: Wallace Gregory Sawyer, Thomas Ettor Angelini, Steven C. Ghivizzani, C. Parker Gibbs
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Patent number: 11363820Abstract: The present invention provides a screening method for selecting plant symbiotic microorganisms enabling plant growth under abiotic stress and/or biotic stress, a microbial mixture of microorganisms which are selected from nature by the method and enables a plant to grow under salinity stress and/or hyperosmotic stress in symbiosis with the plant. Specifically, the present invention provides a screening method for selecting plant symbiotic microorganisms, comprising a first screening step of allowing at least one plant to grow for a predetermined period of time while being partially soaked in a solution containing a microbial mixture under abiotic stress and/or biotic stress, and collecting microorganisms adhering to grown plant to thereby select out the microorganisms as plant symbiotic microorganisms enabling plant growth under the stress.Type: GrantFiled: December 8, 2017Date of Patent: June 21, 2022Assignee: SEKISUI CHEMICAL CO., LTD.Inventor: Seigo Ono
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Patent number: 11365388Abstract: Provided is a method of producing a novel microorganism having a minimal genome. In particular, provided are a method of producing a novel microorganism having a smaller genome size than a wild-type, the method including the step of culturing the microorganism in a medium containing LB broth with stepwise decreasing concentrations of the LB broth; and a novel microorganism having a smaller genome size than a wild-type, produced by the above method. Despite the small genome size, the novel microorganism of the present disclosure may have a high growth rate, pyruvate production capacity, and protein translation efficiency thereby being usefully applied to industry such as production of recombinant proteins or pyruvate.Type: GrantFiled: January 24, 2020Date of Patent: June 21, 2022Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sun-Chang Kim, Byung-Kwan Cho, Donghui Choe, Bong Hyun Sung, Jun Hyoung Lee
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Patent number: 11345886Abstract: The presently disclosed subject matter relates, in general, to the identification, isolation, and use of a population of stem cells isolated from umbilical cord blood, peripheral blood and/or other sources and that are referred to herein as Small Mobile Stem cells (short: SMS). More particularly, the presently disclosed subject matter relates to isolating said SMS stem cells and employing the same, optionally after in vitro manipulation, to treat tissue and/or organ damage in a subject in need thereof.Type: GrantFiled: July 18, 2018Date of Patent: May 31, 2022Assignee: SMSBIOTECH, INC.Inventor: Abdulkader Rahmo
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Patent number: 11332712Abstract: A cell culture medium comprising adenosine triphosphate; a carrier protein; cholesterol, linoleic acid, and lipoic acid; glutathione; at least one nucleotide salvage pathway precursor base; phosphoethanolamine; selenium; transferrin; triiodothyronine; all-trans-retinoic acid (ATRA) and vitamin C; zinc, magnesium, and copper; an agent that increases intracellular cAMP; epidermal growth factor (EGF); hydrocortisone; insulin; and charcoal stripped fetal bovine serum, wherein said cell culture medium is substantially free, if not entirely free, of vitamin D, androgenic hormones, androgenic ligands, estrogenic hormones, estrogenic ligands, and/or androgenic receptors.Type: GrantFiled: October 10, 2019Date of Patent: May 17, 2022Assignee: UNIVERSITY OF MIAMIInventor: Tan Ince
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Patent number: 11332711Abstract: Described herein are compositions and methods to cryopreserve sporozoites. In some aspects, the method can include the step of placing harvested salivary glands containing sporozoites into an insect-based medium.Type: GrantFiled: April 5, 2019Date of Patent: May 17, 2022Assignee: UNIVERSITY OF SOUTH FLORIDAInventors: John H. Adams, Alison Elizabeth Roth