Abstract: Provided are methods for producing food products comprising recombinant components, and compositions used in and food products produced by such methods.
Type:
Grant
Filed:
April 23, 2021
Date of Patent:
May 14, 2024
Assignee:
Perfect Day, Inc.
Inventors:
Timothy Geistlinger, Heather Jensen, Ravirajsinh Jhala, Hendrik Meerman, Balakrishnan Ramesh, Ty Wagoner, Timothy Scott Johnson, Vincent Wei-Xiang Wu, Francesca Manea
Abstract: A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
Abstract: The present invention relates to methods for producing oxygenated terpenoids. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided.
Type:
Grant
Filed:
January 5, 2021
Date of Patent:
April 9, 2024
Assignee:
Manus Bio Inc.
Inventors:
Ajikumar Parayil Kumaran, Chin Giaw Lim, Liwei Li, Souvik Ghosh, Christopher Pirie, Anthony Qualley
Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, 1,4-butanediol, or other product pathway and being capable of producing 4-hydroxybutyrate, 1,4-butanediol, or other product, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, 1,4-butanediol, or other product or related products using the microbial organisms.
Type:
Grant
Filed:
June 3, 2013
Date of Patent:
March 19, 2024
Assignee:
Genomatica, Inc.
Inventors:
Priti Pharkya, Anthony P. Burgard, Stephen J. Van Dien, Robin E. Osterhout, Mark J. Burk, John D. Trawick, Michael P. Kuchinskas, Brian Steer
Abstract: Provided herein are gene therapy compositions and methods for treating, preventing, and/or curing NPC1. More specifically, the disclosure provides Adeno-associated virus (AAV) vectors for delivery of nucleic acids and nucleic acids (including AAV transfer cassettes) for treating, preventing, and/or curing NPC1.
Type:
Grant
Filed:
October 16, 2020
Date of Patent:
February 20, 2024
Assignee:
Ginkgo Bioworks, Inc.
Inventors:
Daniel McCoy, Garrett E. Berry, David Dismuke
Abstract: The present disclosure relates to the technical field of application of bioengineering technology in microbial oil recovery, and discloses a Brevibacillus agri strain and preparation thereof and a method for preparing surfactant, and use thereof. The Brevibacillus agri strain is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No. 9983. The Brevibacillus agri and its preparation may effectively enhance the crude oil recovery; the method for preparing the surfactant allow the lipopeptide biosurfactant to have good physical properties, effectively reduce the surface tension, and have good emulsifying performance for petroleum, various hydrocarbons and lipids.
Type:
Grant
Filed:
May 11, 2021
Date of Patent:
January 23, 2024
Assignee:
Yangtze University
Inventors:
Yuehui She, Fan Zhang, Zhi Zhang, Puyong Yao, Fei Li, Hao Dong, Shanshan Sun, Gaoming Yu, Shaojin Yi, Wenda Zhang, Linqi Hu, Yangyang Feng, Anying Zheng, Yang Li
Abstract: Provided herein are engineered DNA polymerases comprising modifications improving accuracy and processivity of the polymerase including modifications in the Motif A region, optionally, along with additional modifications in the palm and/or exonuclease domains of the polymerase. Also provided are nucleic acids encoding the engineered DNA polymerases comprising modifications in motif A of the polymerase, optionally, with additional modifications. Methods, vectors, kits, and compositions comprising the nucleic acids and compositions, methods and kits comprising the engineered polymerases are also provided.
Type:
Grant
Filed:
February 8, 2022
Date of Patent:
January 16, 2024
Assignee:
PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
Inventors:
Pinar Iyidogan, Mariam Iftikhar, Bridget Kidd, Lewis Churchfield
Abstract: The disclosure provides methods of manufacturing products comprising allulose produced by contacting a protein having allulose 3-epimerase activity with a fructose substrate under conditions such that the fructose substrate is converted into allulose. The disclosure also provides methods of manufacturing products comprising allulose produced by providing a vector comprising a nucleic acid molecule having a polynucleotide sequence encoding a protein having allulose 3-epimerase activity.
Type:
Grant
Filed:
December 31, 2020
Date of Patent:
January 2, 2024
Assignee:
Tate & Lyle Solutions USA LLC
Inventors:
Ryan David Woodyer, Richard W. Armentrout
Abstract: Disclosed are an antimycin compound and a preparation method and use thereof. The preparation method comprises: fermenting a marine actinomycete (Streptomyces sp.4-7) with a preservation number CCTCCNO: M2020953 to obtain a fermented product and soaking and extracting the fermented product with ethyl acetate to obtain a crude extract; and carrying out separation and purification by normal-phase silica gel column chromatography, reversed-phase MPLC, and semi-preparative reversed-phase high-performance liquid chromatography. The invention has the advantages of resistance against Botrytis cinerea and Penicillium citrinum.
Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.
Type:
Grant
Filed:
March 20, 2018
Date of Patent:
December 19, 2023
Assignee:
NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
Abstract: Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.
Type:
Grant
Filed:
June 15, 2021
Date of Patent:
November 21, 2023
Assignee:
CARGILL, INCORPORATED
Inventors:
Arlene M. Fosmer, Vernon L. McIntosh, Jr., Thomas W. McMullin, Gregory M. Poynter, Brian J. Rush, Kevin T. Watts
Abstract: The invention provides an animal feed composition comprising microbial ?-amylase. The invention further provides methods of increasing the growth (weight gain), the average daily weight gain or the efficiency of feed utilization by an animal or reducing the number of days needed to achieve a desired weight in an animal, comprising feeding to the animal an animal feed composition of the present invention.
Type:
Grant
Filed:
October 27, 2020
Date of Patent:
November 7, 2023
Inventors:
David Witherspoon, Tammiraj Kumar Iragavarapu
Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
Type:
Grant
Filed:
February 15, 2022
Date of Patent:
November 7, 2023
Assignee:
Codexis, Inc.
Inventors:
Robert Osborne, Vesna Mitchell, Khin Yu Naing Htwe, Xiyun Zhang, Erika M. Milczek, Jeffrey C. Moore
Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.
Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Type:
Grant
Filed:
May 6, 2022
Date of Patent:
October 31, 2023
Assignee:
Codexis, Inc.
Inventors:
Oscar Alvizo, Melissa Ann Mayo, Jeffrey C. Moore
Abstract: An enzyme synthesizes hydroxyl acetaldehyde and/or 1,3-dihydroxyacetone by catalyzing formaldehyde. Site-directed mutation of benzoylformate decarboxylase (BFD) creates a mutant of the enzyme, which can polymerize the formaldehyde, A phosphoketalose (F/XPK) generates acetyl phosphoric acid from the hydroxyl acetaldehyde or 1,3-dihydroxyacetone (DHA). Combination with phosphotransacetylase (Pta) provides a route from the formaldehyde to acetyl coenzyme A in three steps.
Type:
Grant
Filed:
February 12, 2018
Date of Patent:
October 31, 2023
Assignee:
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
Abstract: Direct detection of mutagenesis in prokaryotes by reversion of an inactivating mutation (reversion mutation assay), producing a quantitative signal for in vivo mutagenesis, may greatly reduce the amount of test chemicals and labor involved in these assays. Further, transcriptional coupling of ?-lactamase reversion and GFP, translational fusion between ?-lactamase and GFP with stop codon in GFP, and a novel dual reporter to monitor continuous mutagenesis may be used in methods described herein.
Type:
Grant
Filed:
April 25, 2022
Date of Patent:
October 17, 2023
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
Type:
Grant
Filed:
December 3, 2021
Date of Patent:
October 10, 2023
Assignee:
DSM IP ASSETS B.V.
Inventors:
Viktor Marius Boer, Priscilla Zwartjens, Johannes Gustaaf Ernst Van Leeuwen