Abstract: Direct detection of mutagenesis in prokaryotes by reversion of an inactivating mutation (reversion mutation assay), producing a quantitative signal for in vivo mutagenesis, may greatly reduce the amount of test chemicals and labor involved in these assays. Further, transcriptional coupling of ?-lactamase reversion and GFP, translational fusion between ?-lactamase and GFP with stop codon in GFP, and a novel dual reporter to monitor continuous mutagenesis may be used in methods described herein.
Type:
Grant
Filed:
April 25, 2022
Date of Patent:
October 17, 2023
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
Type:
Grant
Filed:
December 3, 2021
Date of Patent:
October 10, 2023
Assignee:
DSM IP ASSETS B.V.
Inventors:
Viktor Marius Boer, Priscilla Zwartjens, Johannes Gustaaf Ernst Van Leeuwen
Abstract: The present invention provides a method for efficiently producing ethanol from a lignocellulosic raw material. More specifically, the present invention provides a method for producing ethanol from a lignocellulosic raw material, which comprises a step of performing multiple parallel fermentation while continuously or intermittently adding an additional saccharification enzyme to a fermentation liquid comprising a lignocellulosic raw material, a saccharification enzyme and a yeast so that the physical property value of the fermentation liquid itself is maintained within a preset range.
Abstract: The present application relates to a method of producing drimenol and/or drimenol derivatives by comprising contacting at least one polypeptide with farnesyl diphosphate (FPP). The method may be performed in vitro or in vivo. Also provided are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce drimenol and/or derivatives of drimenol.
Type:
Grant
Filed:
August 20, 2021
Date of Patent:
October 3, 2023
Assignee:
FIRMENICH SA
Inventors:
Pan Li, Qi Wang, Xiu-Feng He, Olivier Haefliger
Abstract: The present invention relates to compositions such as cleaning compositions comprising enzymes. The invention further relates, use of compositions comprising such enzymes in cleaning processes.
Type:
Grant
Filed:
October 31, 2018
Date of Patent:
September 26, 2023
Assignee:
Novozymes A/S
Inventors:
Rebecca Munk Vejborg, Dorotea Raventos Segura, Jesper Salomon, Johanne M. Jensen, Rune Nygaard Monrad, Anne Vindum Due, Martin Gudmand
Abstract: ?2-6-Sialyltransferase (2,6ST) variants having improved ?2-6-specific sialidase activity as compared to the native 2,6ST enzymes are described. The variants include GT80 sialyltransferases such as P. damselae Pd2,6ST. Methods for making de-sialylated products and screening sialidase activity are also described.
Type:
Grant
Filed:
December 21, 2018
Date of Patent:
August 29, 2023
Assignee:
The Regents of the University of California
Inventors:
Xi Chen, John B. McArthur, Andrew J. Fisher
Abstract: Described is a method for the preparation of a gelatin hydrolysate having a decreased endotoxin content, comprising the steps of incubating a solution of gelatin of gelatin hydrolysate at a temperature of 70-125° C. at a pH of 3.5 or less for a time period of at least 15 minutes, and recovering the gelatin hydrolysate. Further a gelatin hydrolysate thus obtained is described.
Type:
Grant
Filed:
November 22, 2018
Date of Patent:
August 15, 2023
Assignee:
Rousselot BVBA
Inventors:
Joseph Hubertus Olijve, Eline Bakhuizen, Paul Stevens
Abstract: Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.
Type:
Grant
Filed:
January 28, 2021
Date of Patent:
August 8, 2023
Assignees:
Battelle Memorial Institute, National Technology & Engineering Solutions of Sandia, LLC
Inventors:
Scott E. Baker, Jon K. Magnuson, Morgann C. Reilly, Joonhoon Kim, John Gladden, Jed J. Lynn
Abstract: The invention relates to a recombinant Escherichia coli expressing a fusion protein of formamidase and phosphite dehydrogenase, a construction method and use thereof. The invention includes adopting engineered E. coli DH5? as a host, amplifying a cloned formamidase gene and a cloned phosphite dehydrogenase gene into a fusion gene, ligating the fusion gene to a multiple cloning site of a vector, transforming the obtained recombinant plasmid into the E. coli DH5?, extracting the plasmid and transforming into an expression strain, and performing induction culture to obtain a recombinant E. coli. The recombinant E. coli can express a fusion protein of formamidase and phosphite dehydrogenase.
Abstract: The present application relates to the technical field of genetic engineering, and provides a monooxygenase mutant, a preparation method and application thereof. The monooxygenase mutant has any one of the amino acid sequences shown in (I) and (II): (I) an amino acid sequence having at least 80% identity with the amino acid sequence shown in SEQ ID NO. 1; and (II) an amino acid sequence obtained by modifying, substituting, deleting, or adding one or several amino acids to the amino acids at 23 to 508 positions of the amino acid sequence shown in SEQ ID NO. 1, the substituting referring to a substitution of 1 to 34 amino acids, wherein the mutant has the activity of monooxygenase.
Abstract: The invention relates to fusion polypeptides, nucleic acid molecules encoding said fusion polypeptides and genetically modified cells including said nucleic acid molecules. Moreover, the invention relates to a method for preparing target polypeptides using the fusion polypeptides.
Abstract: The present disclosure relates to a variant of cAMP receptor protein of Escherichia coli with alanine at position 35, a microorganism including the same, and a method of producing an L-amino acid using the same.
Type:
Grant
Filed:
July 25, 2019
Date of Patent:
July 11, 2023
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Seok Myung Lee, Ki Yong Cheong, Chang Il Seo, Ji Sun Lee
Abstract: This disclosure provides methods and landing pad constructs for generation of parental cell lines suitable for targeted integration. A method is provided by the parental cell line development; this is, the introduction of binding sites of BPV1 E2 protein to landing pad vectors so that expressed BPV1 E2 protein could locate the vector to transcriptionally active region in the genome. Cells with high expression level of reporter genes are selected for the next stage and will be used in the development of cell lines expressing another recombinant protein by recombination mediated cassette exchange (RMCE). Landing pad constructs include recombination target sites for site-specific recombinases, and therefore, it could be replaced with gene-of-interest expression construct containing the same set of recombination target sites. This yields the generation of producer cell lines with less effort compared to traditional cell line development by random integration.
Type:
Grant
Filed:
October 17, 2019
Date of Patent:
June 27, 2023
Assignee:
Icosagen Cell Factory OÜ
Inventors:
Kadri Õunap, Eva-Maria Tombak, Mart Toots, Madis Jakobson, Mart Ustav, Jr., Kerttu Murumets, Urve Toots, Andres Männik, Mart Ustav, Sr.
Abstract: Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins in E. coli host strains. Also provided are E. coli host strains with improved capacity for producing recombinant proteins.
Type:
Grant
Filed:
September 4, 2018
Date of Patent:
June 6, 2023
Assignee:
SCARAB GENOMICS, LLC
Inventors:
Frederick R. Blattner, Robert E. Novy, David A. Frisch, Charles Landry, Hyunsic Choi, Eric A. Steffen, John Brandon
Abstract: Disclosed herein is a process for producing a recombinant Candida cell, which involves genetically engineering a parent Candida cell using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas)(CRISPR/Cas) system. A recombinant Candida cell obtained using the process and a method for producing D-lactic acid from a biomass using the recombinant Candida cell are also disclosed.
Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
Type:
Grant
Filed:
September 3, 2020
Date of Patent:
May 30, 2023
Assignee:
PROMEGA CORPORATION
Inventors:
Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood, Dieter Klaubert, Poncho Meisenheimer, James Unch, Michael P. Valley
Abstract: The present disclosure discloses Microbacterium oleivorans capable of degrading polyethylene terephthalate and an intermediate thereof, and belongs to the technical field of microorganisms. The present disclosure provides Microbacterium oleivorans JWG-G2 capable of degrading the polyethylene terephthalate. After Microbacterium oleivorans JWG-G2 is inoculated into an inorganic salt liquid medium containing 2 g/L polyethylene terephthalate plastic particles with an inoculation quantity of 1×108 CFU/mL to be cultivated for 5 d, the polyethylene terephthalate plastic particles can be partially degraded into monohydroxyethyl terephthalate and terephthalic acid capable of being directly recycled, ester bond functional groups on surfaces of the polyethylene terephthalate plastic particles can be reduced, and a weight loss ratio of the polyethylene terephthalate plastic particles can reach 5.6%.
Abstract: The present invention relates to a coupled biotransformation process of converting chenodeoxycholic acid (CDCA) and related compounds to ursodeoxycholic acid (UDCA) and related compounds. It also relates to the cloning, expression, and biochemical characterization of a novel NADP+-dependent 7?-hydroxysteroid dehydrogenase (7?-HSDH) from Clostridium difficile, cofactor switch mutants thereof, and their application for the oxidation of bile acids. A further aspect of the invention relates to novel NADP-dependent cofactor switch mutants of the NADP+-dependent 7?-HSDH of E. coli and their application for the oxidation of bile acids.
Type:
Grant
Filed:
June 19, 2017
Date of Patent:
April 25, 2023
Assignee:
Pharmazell GmbH
Inventors:
Daniel Bakonyi, Werner Hummel, Ralf Gross
Abstract: Provided are an L-glutamate dehydrogenase mutant and an application thereof, the mutant mutating the amino acid residue A at position 166 and/or the amino acid residue V at position 376 shown in SEQ ID NO. 1 into a hydrophilic or small sterically hindered amino acid residue, the application performing an amination reaction of 2-oxo-4-(hydroxymethylphosphinyl)butyrate in the presence of an L-amino acid dehydrogenase mutant, an inorganic amino donor, and a reduced coenzyme NADPH, and performing an acidification reaction on the obtained L-glufosinate salt to obtain L-glufosinate. Compared to wild L-glutamate dehydrogenase, the present L-glutamate dehydrogenase mutant has a higher concentration of substrates that can be catalysed when preparing L-glufosinate, thereby increasing the efficiency of the action of the enzyme and reducing reaction costs.
Type:
Grant
Filed:
April 3, 2019
Date of Patent:
April 25, 2023
Assignee:
ABIOCHEM BIOTECHNOLOGY CO., LTD
Inventors:
Zhenhua Tian, Zhanbing Cheng, Shaonan Ding, Wenxuan Xu, Ruru Wang, Qi Jiao, Yao Huang
Abstract: Disclosed herein are genetically modified microorganisms and related methods for enhanced utilization of oligosaccharides and improved productivity of compounds derived from the metabolism of the oligosaccharides. The microorganisms described herein have altered activities of plasma membrane ATPase protein (PMA1) and/or one or more extracellular glucose sensors, namely, sucrose non-fermenting protein (SNF3), restores glucose transport protein (RGT2), and G protein-coupled receptor 1 protein (GPR1). These genetic modifications provide the microorganisms an increased ability to utilize an oligosaccharide to produce a compound of interest, particularly, tagatose, 2?-fucosyllactose, and psicose. Methods of culturing the microorganisms in the presence of such oligosaccharides to produce the products of interest are also provided.
Type:
Grant
Filed:
June 29, 2018
Date of Patent:
March 7, 2023
Assignee:
Zimitech, Inc.
Inventors:
Kulika Chomvong, James Harrison Doudna Cate, Yong-Su Jin