Abstract: Disclosed herein are methods and system for formation fluid sampling. In at least some embodiments, the method includes pumping formation fluid from a public flow line of a downhole tool via a private flow line into a detachable sample chamber. The method also includes isolating the private flow line from the public flow line. The method also includes collecting measurements of the formation fluid in the private flow line. The method also includes associating the measurements with the detachable sample chamber.

Abstract: Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio “?” value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.

Abstract: A substrate assembly includes a first substrate, a second substrate and a bonding member. The first substrate includes a first surface-modified region having a functionality different from that of a remainder region of the first substrate. The second substrate includes a second surface-modified region connected to the first surface-modified region through a physical interaction and having a functionality different from that of a remainder region of the second substrate. The first and second substrates cooperatively define a space therebetween. The bonding member is disposed within said space to bond said first and second substrates together. A method for bonding substrates is also disclosed.

Abstract: A vial and system are configured to simplify fluid input and extraction therefrom. The vial has a first end opposite a second end and with a collapsible sidewall. The sidewall can be collapsed, such as by imparting a differential pressure between an interior and exterior of the vial, for simplified fluid extraction and smooth extraction without disturbing boundaries between separated components. The vial typically has a bulb at a first end and a neck extending away from the bulb and toward the second end. An inlet spaced from the first end can be configured to attach to various different interfaces or processing elements for input and output of fluids. A support cup can be provided for use with the vial during centrifugation to allow the vial to withstand any centrifugation forces. Various fluid processing methodologies are supported by the collapsible vial, especially including methods of separation of components of blood.

Abstract: The present invention relates to compositions comprising a metal, a metal aggregation inhibitor, and a colour changing agent. The metal is bindable to the colour changing agent to provide a change in colour on binding and/or release thereof.

Abstract: Methods of extracting DNA from soil involve lysing microbial cells contained within the soil by mixing it with an extraction buffer containing cetrimonium bromide (cTAB). The cTAB helps reduce or eliminate high levels of humic acid often present within the soil, which interferes with processes including PCR. Methods further involve binding DNA lysed from the microbial cells and then bound to a silica substrate, washing non-DNA debris from the silica substrate, eluting the DNA from the substrate, and eluting the isolated DNA in an elution buffer. Example methods may involve extracting microbial DNA from a plurality of soil samples. Such methods involve adding soil samples into separate wells within a multi-well plate, lysing microbial cells within the samples using an extraction buffer, binding the microbial DNA released from the cells to silica particles, washing non-DNA debris from the silica particles, and separating the DNA from the silica particles.

Abstract: A method for determining a phosphate concentration in a water sample includes providing the water sample, adding a first acid to the water sample to obtain a second water sample in which a concentration of the first acid is at least 0.5 wt.-%, performing a first photometric measurement of the second water sample, adding a coloring component to the second water sample to obtain a third water sample, performing a second photometric measurement of the third water sample, and calculating the phosphate concentration from a difference between the first photometric measurement and the second photometric measurement.

Abstract: A method includes mounting an integrated electro-microfluidic probe card to a device area on a bio-sensor device wafer, wherein the electro-microfluidic probe card has a first major surface and a second major surface opposite the first major surface. The method further includes electrically connecting at least one electronic probe tip extending from the first major surface to a corresponding conductive area of the device area. The method further includes stamping a test fluid onto the device area. The method further includes measuring via the at least one electronic probe tip a first electrical property of one or more bio-FETs of the device area based on the test fluid.

Abstract: Disclosed is a triboelectric nanogenerator-based biochemical droplet reaction device, which includes a reaction generating part and a power generation part. The power generation part includes a triboelectric component and a rectifier circuit. The triboelectric component includes a drive electrode, a substrate, a first friction electrode, a first friction material, a second friction material, and a second friction electrode arranged in sequence from top to bottom. A gap exists between the first friction material and the second friction material. The first friction electrode is connected to the first friction material. The second friction electrode is connected to the second friction material. The drive electrode, the first friction electrode, and the second friction electrode are all connected to the rectifier circuit. Also disclosed is a reaction method.

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

Abstract: The disclosure relates to methods of manufacturing and using a single layer microfluidic for detecting target analytes, including obtaining a single layer sheet of paper; depositing wax boundaries onto the paper in a plurality of patterns including a main channel, fluid transfer channels, and an independent diagnostic area corresponding to each fluid transfer channel; melting the wax through the paper; depositing diagnostic components onto the diagnostic areas; depositing a continuous wax backing; and cutting devices from the paper. The disclosure also relates to a method of capturing an image of the micro fluidic device to generate diagnostic results corresponding to the diagnostic components by: identifying at least two panels from the image; and determining a color for each panel of the at least two panels; and generating for display, using the computing device, a graphical user-interface including at least one component visualizing the diagnostic results.

Abstract: The present invention relates to a method for diagnosing anomalies in the coagulation of blood, comprising the following successive steps: a) placing a sample of total blood in a holder containing two pairs of electrodes connected to an apparatus generating an electrical current; b) incubating this sample for 60 to 180 seconds in the presence of calcium; c) adding to this sample tissue factor in a concentration high enough to trigger the coagulation of the blood; d) measuring the impedance variation between the electrodes as a function of time, for a period comprised between 10 and 30 minutes from step (c), and generating a curve of the impedance values as a function of time; e) comparing the value of the area under the curve generated in step (d) with the value of an area under a reference curve.

Abstract: Systems and methods for automated sample preparation and processing of protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic agents.

Abstract: An apparatus for generating gas from a sample (battery) by pyrolysis of the sample in order to collect or analyze gas generated inside the sample due to the thermal behaviors of the sample. More specifically, provided is an apparatus wherein not only gas generated due to the thermal behaviors of a sample (battery) can be generated by heating the sample (battery itself), but also a series of processes provided to collect or analyze the generated gas can be automatically controlled.

Abstract: A metal contamination detection system, forming part of a cyanide detection system which is especially useful when used in conjunction with a corrinoid cyanide detection system as it allows for the quick and accurate assessment of impurities that could lead to a false cyanide result, and therefore the need to remove the impurities for an accurate result or a better metals recovery.

Abstract: The present disclosure relates to a kit and method for testing harmful heavy metals onsite, which can determine the presence or absence of harmful heavy metals and the degree of contamination with harmful heavy metals dissolved in water or contained in sediment, soil, deep-sea mining tailings and the like. The kit includes a receiving member and a diagnostic solution containing a biosurfactant, which is accommodated in the receiving member and undergoes an ion exchange reaction with a liquid including harmful heavy metals or a solid sample from which harmful heavy metals are leached in a liquid phase.

Abstract: Systems and methods for the detection of one or more target molecules, such as benzene, are described. The systems and methods may include a molecularly imprinted polymer film; a sensing material, wherein the molecularly imprinted polymer film comprises a polymer host with one or more binding sites for one or more target molecules. The molecularly imprinted polymer film may be coated upon the sensing material.

Abstract: A method for the detection of hydrocarbon contamination in a sample is disclosed. The method includes contacting a sample with a molecular probe. The molecular probe has a photoluminescence which is environmentally sensitive. The photoluminescence from the molecular probe is collected. The method includes determining whether the photoluminescence is indicative of a hydrocarbon contaminated sample. A test strip for the detection of hydrocarbon contamination in a sample is also disclosed. The test strip includes a molecular probe embedded in a substrate and/or immobilized to the substrate, the molecular probe having a photoluminescence which is environmentally sensitive to hydrocarbon contaminated sample.

Abstract: The present invention provides a pipette tip, which can be used in in-vitro diagnostics, in particular in the diagnostic testing of body fluids, such as in coagulation testing. The Pipette tip contains two constituents in a spatially separated manner. The present invention furthermore provides a method of performing such diagnostics, e.g. coagulation analysis, and to the use of the pipette tip in such diagnostic testing.

Abstract: Paraffin-embedded tissue is prepared removing paraffin from the tissue. The paraffin is removed by generating an electric field effective to produce plasma and direct charged species of the plasma to the paraffin, thereby rendering the paraffin responsive to the electric field. The electric field may move the paraffin out from the tissue due to electrostatic force. Movement of the paraffin may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. Movement of the paraffin also may be assisted by applying a solvent and/or heat energy to the tissue.