Abstract: A chromatography column is prepared with a stationary phase comprising a deuterated poly(ethyleneglycol), or other deuterated polymer. Formation of the stationary phase can be performed using exactly the same methodology as used when forming a stationary phase with the equivalent non-deuterated polymer. The deuterated poly(ethyleneglycol), or other deuterated polymer, preferably has increased thermal stability as compared to non-deuterated poly(ethyleneglycol), or equivalent non-deuterated polymer. This reduces bleeding of the stationary phase during gas chromatography and allows the use of greater operating temperatures.

Abstract: A non-particulate organic porous material with optical resolution capability, the non-particulate organic porous material having a continuous pore structure, which comprises macropores and mesopores, the macropores being interconnected forming mesopores with a radius of 0.01-100 ?m in the interconnected parts, and optically active groups uniformly introduced therein possesses high physical stability, can be used under wide separating conditions, and has a large capacity for separating optically active substances (enantiometers).

Abstract: A plate apparatus for use in countercurrent chromatography is disclosed which comprises a disk shaped tube support (60) having a tube support pattern form in an upper surface (63) of the support and configured to accommodate at least one layer of fluid flow tubing (70), wherein the pattern comprises at least one spiral groove (62) and at least one return path (72).

Abstract: Disclosed is a separating agent for optical isomer which is excellent in optical separation ability. Specifically disclosed is a separating agent for optical isomer containing: an inclusion complex including a ?-conjugated polymer in a polymer compound having a hydroxy group or an amino group; and a carrier, the inclusion complex being carried by the carrier.

Abstract: To provide a medium suitable for high speed/high resolution, rich in hydrophilicity and resistant to a high concentration aqueous alkaline solution. A medium comprising crosslinked polymer particles containing from 20 to 95 mol % of repeating units derived from a (meth)acryloyl monomer represented by the formula (1): wherein R2 is a hydrogen atom or a C1-4 alkyl group, R1 is —NR3-R4-R5 or —O—R4-R5, R3 is a hydrogen atom or a C1-4 alkyl group, R4 is a C6-15 alkylene group containing an alicyclic ring or a C4-8 linear alkylene group, and R5 is a halogen atom, an alcoholic OH group, an amino group, a glycidyl group or an epoxy group.

Abstract: In an embodiment, a porous composite particulate material includes a plurality of composite particles. Each composite particle includes an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles. The shell particles are adhered to the core particle by a polymeric layer. The shell particles and/or core particles may be made from an acid-base-resistant material that is stable in harsh chemical conditions. For example, the shell particles and/or core particles may be made from diamond, graphitic carbon, silicon carbide, boron nitride, tungsten carbide, combinations of the foregoing, or other acid-base-resistant materials. The porous composite particulate materials disclosed herein and related methods and devices may be used in separation technologies, including, but not limited to, chromatography, and solid phase extraction.

Abstract: Disclosed are methods for the chromatographic separation of rebaudioside A from stevioside in glycoside solutions that are derived from stevia. The chromatographic separation may be an adsorb/desorb type of chromatographic separation or a fractionation type of chromatographic separation.

Abstract: The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

Abstract: A frit (44) includes a porous support structure having a plurality of void spaces, where a plurality of secondary particles (46) are filled in the void spaces, the secondary particles (46) being dimensioned with respect to the void spaces such that the frit (44) retains packing materials with diameters of less than about 2.5 microns. Preferably one or more frits (44) are received in a high pressure liquid chromatography (HPLC) chromatographic column, where the column includes fittings and filter assemblies for receiving frits (44) at its inlet and outlet. The frit (44) can be constructed from a porous support structure with void spaces or pores that are filled with secondary particles (46) smaller those used to manufacture the support. The particles (46) contained in the void spaces are large enough to be retained by the support, but small enough to create a finer network of interconnected channels within the support's void spaces that are capable of retaining sub-2.5 micrometer particles.

Abstract: Embodiments of the present invention feature methods and apparatus for performing chromatographic separations. The invention feature methods and control means in the form of computer programs and software that control the formation of a concentration gradient at two or more total flow rates, with an elution period occurring in a slow flow rate and at least other portions of the gradient occurring during a fast total flow rate.

Abstract: A chromatography column distribution system (101) comprises a set of first bed support ribs (107) extending radially from a inner, first radial position (R1) near the centre of the plate to a outer radial position nearer to the periphery (109) of the plate and at least one set of intermediate bed support ribs (117, 119) starting at an intermediate radial position (R2, R3) and extending to an outer radial position nearer to the periphery (109) of the plate (101), whereby channels are formed between adjacent bed support ribs (107, 117, 119).

Abstract: A device and method for making a microfluidic separation device. A microfluidic separation device could include a microfluidic column having an inlet, the microfluidic column being configured to hold a first fluid and the microfluidic column including a porous portion, and an outlet attached to the microfluidic column, the outlet being configured to output a second fluid. The method may include providing a microfluidic column having an inlet, configuring the microfluidic column to hold a first fluid, forming a porous portion in the microfluidic column, and attaching an outlet to the microfluidic column.

Abstract: A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syringe, or other analytical or sample preparation instrument.

Abstract: A solution containing a target compound is passed through a trap column (14) to capture the target compound in the column (14). Thereafter, wash water is introduced in the trap column (14). Then, the setting of a six-port valve (7) is changed and an on/off valve (6) is opened to introduce nitrogen gas through a supply-side passage (8) in the trap column (14). In this stage, the nitrogen gas is warmed by means of a heat-exchange block (10), and the trap column (14) is warmed via a column rack (15). Any water remaining in the trap column (14) is initially thrust upwards by the nitrogen gas, to be extruded from an exit edge (14b) and discharged through a discharge-side passage (20). Furthermore, any water adhering to adsorbent or the inner wall surface of the trap column (14) is quickly vaporized and carried away by the nitrogen gas.

Abstract: A mixed liquid passage (31) is connected to a trapping passage (22) at a point upstream of a trap column (23). A mixture of a diluting liquid and an additive agent, which are respectively drawn and sent by pumps (35) and (36), is supplied into the mixed liquid passage (31). When an eluate containing a target compound is passed through the trap column (23), the eluate is diluted with the diluting liquid and hence its elution capability decreases, so that the target compound can be easily captured in the trap column (23). Meanwhile, the solubility of the mobile phase in the eluate decreases, causing not only the target component but also foreign compounds to more easily precipitate. However, their precipitation is prevented by the effect of the additive agent. Thus, clogging of the trap column (23) or pipes is prevented.

Abstract: Methods and related apparatuses and mixtures are described for chromatographic analysis. The described system includes a pressurized source of a mobile phase and a flow path in fluid communication with the pressurized source such that the mobile phase flows through the flow path. The system also includes an injector in fluid communication with the flow path and downstream of the pressurized source, the injector being configured to inject a sample into the flow path. A first column located downstream of the injector, contains a stationary phase, and forms part of the flow path. A first detector is positioned to detect properties of fluid in the flow path at a location downstream of the injector and upstream from the first column. A second detector is positioned to detect properties of fluid in the flow path at a location downstream of the first column.

Abstract: The present is a hydrophilic polymer microparticle, which has a DW/DA ratio of 2.0 or less, the DW representing a particle diameter of the hydrophilic polymer microparticle dispersed in water, the DA representing a particle diameter of the hydrophilic polymer microparticle dispersed in acetone, each of the particle diameters measured by a particle size distribution analyzer after the hydrophilic polymer microparticle is dispersed in water or acetone, subjected to irradiation with ultrasonic waves for 15 minutes, and allowed to stand at a temperature of 25° C. for 240 hours for equilibration; and has a contact angle with water of 70° or less, the contact angle measured using a contact angle measurement apparatus at a temperature of 25° C. by forming a droplet of pure water on a single layer of the hydrophilic polymer microparticles arranged with no space therebetween.

Abstract: Aimed at readily and exactly separate 8-hydroxy-2?-deoxyguanosine (8-OHdG) contained in a sample, a column packing material used for separating 8-OHdG, which contains a packing material composed of a material having a straight-chain hydrocarbon group having 6 or more and 30 or less carbon atoms as a functional group, and having a carbon content over the surface of carrier of 18% or less by element ratio, wherein the packing material contains 1 cumulative percent or more and 20 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 0.5 ?m or larger and 10 ?m or smaller, is used.

Abstract: A method of liquid chromatography includes providing one or more solvent reservoirs, providing a solvent pump, drawing one or more solvents into the pump in response to a pressure drop that promotes outgassing of the solvents, and dispersing outgassed bubbles into smaller bubbles to promote re-dissolution of the gas. A liquid-chromatography apparatus includes at least two solvent reservoirs, a pump, at least one bubble-dispersing unit that receives a pressurized flow of proportioned solvents from the pump, and a control unit. The control unit includes a processor and a memory that stores instructions; the control unit controls proportioning of solvents to obtain a preselected solvent composition, and pumping at flow rates to support preparative-scale or process-scale liquid chromatography.

Abstract: A single pass simulated moving bed apparatus and methods are described for continuously separating a target molecule from a liquid mixture, using a simulated moving bed system. The simulated moving bed system includes a plurality of filter cartridge modules in serial fluid communication. Each filter cartridge module includes a volume of stationary phase particulates adjacent a porous substrate layer. The volume of stationary phase particulates has a bed height of less than 1 centimeter.