Abstract: Geranyl pyrophosphate (GPP) is a key intermediate molecule in the bioproduction of thousands of natural products. Currently, natural products are either cultivated from plants, synthesized via complex chemical synthesis strategies, or through cell-based factories also known as biofoundries. However, in order to replicate the process in a cell free environment, numerous enzymes and cofactors must be utilized making this approach costly and unviable. In order to make this process viable, a new approach was needed that uses fewer enzymes and co-factors. As described herein, the present invention demonstrates that it is possible to create GPP from glycerol through a short and concise biosynthetic pathway outside of the cell.
Abstract: The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide.
Type:
Grant
Filed:
June 1, 2022
Date of Patent:
June 4, 2024
Assignee:
ERBER AKTIENGESELLSCHAFT
Inventors:
Sebastian Fruhauf, Michaela Thamhesl, Martin Pfeffer, Dieter Moll, Gerd Schatzmayr, Eva Maria Binder
Abstract: The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide.
Type:
Grant
Filed:
June 1, 2022
Date of Patent:
June 4, 2024
Assignee:
ERBER AKTIENGESELLSCHAFT
Inventors:
Sebastian Fruhauf, Michaela Thamhesl, Martin Pfeffer, Dieter Moll, Gerd Schatzmayr, Eva Maria Binder
Abstract: The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Type:
Grant
Filed:
June 2, 2021
Date of Patent:
May 21, 2024
Assignee:
Danisco US Inc.
Inventors:
Marc Kolkman, Rie Mejldal, Anja Hemmingsen Kellett-Smith, Lilia Maria Babe
Abstract: The present invention relates to a process of producing a monoterpene and/or derivatives thereof. The process comprises the steps of: a) providing a host microorganism genetically engineered to express a bacterial monoterpene synthase (mTS); and b) contacting geranyl pyrophosphate (GPP) with said bacterial mTS to produce said monoterpene and/or derivatives thereof. The present invention also relates to a microorganism for use in producing a monoterpene and/or derivatives thereof and a recombinant microor-ganism adapted to conduct the step of converting geranyl pyrophosphate (GPP) into a monoterpene and/or derivatives thereof by ex-pression of a bacterial mTS. It was shown to produce 1,8 cineole using 1,8 cineole synthase and to produce linalool using linalool synthase, both from Streptomyces clavuligerus.
Type:
Grant
Filed:
April 23, 2021
Date of Patent:
May 14, 2024
Assignee:
C3 BIOTECHNOLOGIES LIMITED
Inventors:
Vijaykumar Karuppiah, Nicole G. H. Leferink, Nigel S. Scrutton
Abstract: The disclosure relates to an enzymatic preparation method of inclusion complexes of tributyrin, and belongs to the technical field of oil microencapsulation. The disclosure combines enzymatic synthesis of cyclodextrin and inclusion of tributyrin with cyclodextrin, including enzymatic preparation of cyclodextrin with a CGT enzyme. Tributyrin is added in the preparation process; after reaction, Tween is added, and homogenization and spray drying are carried out. The effect of the finally obtained tributyrin powder is much better than that of single inclusion of tributyrin with cyclodextrin. The disclosure is simple in process, low in cost and convenient in operation; reaction processes are free of toxicity and pollution; there are no toxic reagent residues; the inclusion effect is obvious, and better utilization of a nutritional additive tributyrin in actual production is facilitated.
Type:
Grant
Filed:
September 28, 2020
Date of Patent:
April 23, 2024
Assignee:
JIANGNAN UNIVERSITY
Inventors:
Caiming Li, Zhaofeng Li, Shuangdi Chen, Zhengbiao Gu, Yan Feng, Zexi Li, Li Cheng, Yan Hong
Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
Type:
Grant
Filed:
July 21, 2022
Date of Patent:
April 23, 2024
Assignee:
Codexis, Inc.
Inventors:
Jack S. Liang, Stephan Jenne, Emily Mundorff, Rama Voladri, James J. Lalonde, Gjalt W. Huisman
Abstract: A saccharification reaction mixture can saccharify at least one of cellulose and hemicellulose and contains at least one of cellulose and hemicellulose, a saccharification enzyme, silica or a silica-containing substance, and at least one compound (A) selected from the group consisting of a polyhydric alcohol compound represented by the following formula (1) or a derivative thereof and an acetylene glycol represented by formula (2) or an alkylene oxide adduct thereof.
Abstract: The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32° C. or greater.
Type:
Grant
Filed:
March 30, 2022
Date of Patent:
March 26, 2024
Assignee:
CARGILL, INCORPORATED
Inventors:
Chris Miller, Brian Rush, Joshua Dunn, Brynne Stanton
Abstract: The present disclosure relates to a composition for preparing hexanol or butanol and a method for preparing hexanol or butanol using ethanol and synthesis gas, wherein the composition according to an aspect of the present disclosure is a medium composition containing ethanol as an active ingredient, and by culturing a strain producing hexanol or butanol after inoculating with a medium containing the composition and supplying synthesis gas, hexanol or butanol can be prepared economically using inexpensive synthesis gas, and hexanol or butanol can be prepared with high efficiency by focusing the flow of a carbon source consumed in a fermentation process to the production of hexanol or butanol.
Type:
Grant
Filed:
June 24, 2022
Date of Patent:
March 19, 2024
Assignee:
KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventors:
Hyun Ju Oh, Youngsoon Um, Sun Mi Lee, Gyeongtaek Gong, Ja Kyong Ko
Abstract: A method for producing a carotenoid or apocarotenoid is disclosed. The method comprises the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter. A host cell comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter is also provided together with a kit.
Type:
Grant
Filed:
February 26, 2018
Date of Patent:
March 5, 2024
Assignee:
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventors:
Congqiang Zhang, Xixian Chen, Heng-Phon Too
Abstract: The disclosure in some aspects relates to recombinant adeno-associated viruses having nuclease grafted to one or more capsid proteins. In some aspects, the disclosure relates to isolated AA V capsid proteins having terminally grafted nucleases and isolated nucleic acids encoding the same. Recent approaches to delivering nucleases to cells for gene editing have focused on delivering of expression vectors engineered to express the nucleases in target cells. However, these approaches have proved to be problematic in many instances due to genotoxicity resulting from to prolonged expression of gene editing system in vivo. To prevent such off-target genotoxicity due to prolonged presence of a gene editing system, several studies explored delivery of mRNA or protein instead of delivering the gene coding for the nucleases in cell culture.
Type:
Grant
Filed:
January 29, 2020
Date of Patent:
March 5, 2024
Assignee:
University of Massachusetts
Inventors:
Guangping Gao, Phillip D. Zamore, Dan Wang
Abstract: The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide.
Type:
Grant
Filed:
December 20, 2021
Date of Patent:
February 27, 2024
Assignee:
ERBER AKTIENGESELLSCHAFT
Inventors:
Sebastian Fruhauf, Michaela Thamhesl, Martin Pfeffer, Dieter Moll, Gerd Schatzmayr, Eva Maria Binder
Abstract: Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
Type:
Grant
Filed:
March 6, 2023
Date of Patent:
February 27, 2024
Assignee:
BASF SE
Inventors:
Brian Michael Green, Michelle Lorraine Gradley
Abstract: The present invention discloses a genus of insect inhibitory proteins that exhibit properties directed to controlling Lepidopteran and/or Hemipteran crop pests, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.
Type:
Grant
Filed:
January 4, 2022
Date of Patent:
February 27, 2024
Assignee:
Monsanto Technology LLC
Inventors:
David J. Bowen, Catherine Chay, Artem Evdokimov, Stanislaw Flasinski, Uma R. Kesanapalli, Megan N. Schroder, Rachael N. Slightom, Nengbing Tao, Andrew M. Wollacott
Abstract: Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
Type:
Grant
Filed:
November 18, 2021
Date of Patent:
February 20, 2024
Assignee:
BASF SE
Inventors:
Brian Michael Green, Michelle Lorraine Gradley
Abstract: Glycerol is a byproduct of biodiesel and bioethanol production and its conversion to value-added chemicals is a promising avenue for realization of the biorefinery concept Conversion of glycerol to pyruvate through glycerate yields pyruvate, is a common intermediate of many high-value natural products. The present invention aims at improving the specific activity of a naturally occurring enzyme toward conversion of glycerate to pyruvate (TvDHT). The present invention features compositions of isolated dehydratase enzyme (DHT) polypeptide composition, in particular the DHT polypeptide composition comprising a single point mutation that increases the specific activity of the enzyme as compared to wild type.
Abstract: A method for modifying double stranded DNA (dsDNA) employing an RNA guided DNA endonuclease to generate two double strand breaks in the dsDNA molecule to be modified, and replacement of the sequence positioned between the double strand breaks with a substitute DNA sequence using the non-homologous end joining (NHEJ) pathway, and corresponding kits and compositions for modifying double stranded DNA molecules.
Type:
Grant
Filed:
December 21, 2018
Date of Patent:
February 6, 2024
Assignee:
Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft