Abstract: The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.
Abstract: Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.
Type:
Grant
Filed:
September 19, 2008
Date of Patent:
August 15, 2023
Assignee:
AMYRIS, INC.
Inventors:
Hiroko Tsuruta, Jacob R. Lenihan, Rika Regentin
Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
Type:
Grant
Filed:
July 29, 2021
Date of Patent:
July 18, 2023
Assignee:
TOYOTA JIDOSHA KABISHIKI KAISHA
Inventors:
Junji Ito, Toru Onishi, Nobuki Tada, Rie Hirao
Abstract: This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
Type:
Grant
Filed:
July 29, 2021
Date of Patent:
July 18, 2023
Assignee:
TOYOTA JIDOSHA KABUSHIKI KAISHA
Inventors:
Junji Ito, Toru Onishi, Nobuki Tada, Rie Hirao
Abstract: The disclosure provides polypeptides comprising an engineered p27, or a fragment thereof. Such polypeptides may be used to form trimeric protein complexes with a cyclin-dependent kinase 4 (Cdk4) (or a variant thereof) or Cdk6 (or a variant thereof), and a cyclin D (CycD) or a variant thereof.
Type:
Grant
Filed:
April 10, 2019
Date of Patent:
July 4, 2023
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: The subject of this invention is improvements in the yield and titer of biological production of muconic acid by fermentation. Increased activity of one or more enzymes involved in the muconic acid pathway leads to increased production of muconic acid.
Type:
Grant
Filed:
May 21, 2021
Date of Patent:
June 27, 2023
Assignee:
PTT GLOBAL CHEMICAL PUBLIC COMPANY LIMITED
Inventors:
Ryan Sillers, Theron Hermann, Michelle Spencer, Russell Udani, R. Rogers Yocum
Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.
Abstract: The present invention relates to the field of genetic engineering, particularly to a glucoamylase TIGa15, gene and application thereof. Said glucoamylase comprises the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2 and has the excellent enzymic properties, which can be applied to feed, food, and medicine industries, can be industrially produce with the genetic engineering technics.
Type:
Grant
Filed:
September 30, 2019
Date of Patent:
June 20, 2023
Inventors:
Huiying Luo, Bin Yao, Yujie Guo, Tao Tu, Yuan Wang, Huoqing Huang, Yingguo Bai, Xiaoyun Su, Yaru Wang, Kun Meng
Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.
Type:
Grant
Filed:
June 4, 2018
Date of Patent:
June 6, 2023
Assignee:
DSM IP ASSETS B.V.
Inventors:
Ioannis Papapetridis, Jacobus Thomas Pronk
Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.
Abstract: Drug-inducible, tunable, and multiplexable Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 (Cpf1)-based activators, and methods of use thereof.
Type:
Grant
Filed:
April 23, 2018
Date of Patent:
June 6, 2023
Assignee:
The General Hospital Corporation
Inventors:
Y. Esther Tak, Benjamin Kleinstiver, J. Keith Joung
Abstract: The invention relates to improved acid whey compositions, having an improve lactose content and stability. The invention also relates to processes of making such compositions.
Abstract: The present invention relates to a novel method for the affinity purification of proteins of interest in a single step, based on the lectin activity of the CRD (Carbohydrate Recognition Domain) of a galectin or part of said domain retaining the ability to bind ?-galactosidase derivative.
Type:
Grant
Filed:
May 11, 2017
Date of Patent:
May 30, 2023
Assignees:
UNIVERSITÉ DE LORRAINE, CENTRE HOSPITALIER RÉGIONAL DE NANCY
Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.
Type:
Grant
Filed:
May 31, 2017
Date of Patent:
May 30, 2023
Assignee:
Danisco US Inc.
Inventors:
David Marquez, Michael Stoner, James T. Kellis, Jr., Adam Garske, Joshua Roy Basler, Katherine Augustyn, Priyanka Chandrasekaran, Miles Christopher Scotcher, Lilia Maria Babe, Richard R. Bott, David A. Estell, Gudrun Vogtentanz, Sina Pricelius, Jian Yao
Abstract: Engineered or non-naturally occurring systems and compositions comprising a novel Cas12b and a guide molecule. Also provided include methods of use the systems and compositions, including in treating and diagnosing diseases.
Type:
Grant
Filed:
June 5, 2020
Date of Patent:
May 2, 2023
Assignees:
THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventors:
Feng Zhang, Jonathan Gootenberg, Omar Abudayyeh, Julia Joung, Alim Ladha, Han Altae-Tran, Guilhem Faure
Abstract: A process to produce a protein concentrate from grain, specifically barley protein concentrate (BPC) through mechanical and biochemical intervention while producing multiple sugar streams as co-products. The resulting BPC preferably contains 54%-95% protein derived exclusively from the enzymatically processed barley and has a pH>5.0. The BPC may contain approximately 10% oil, less than 5% crude fiber, less than 1% residual glucose, and less than 0.5% phytic acid. The BPC contains no ethanol, organic acid, fermentation products, or microbial cells or cell mass. No fermentation occurs in the production of the BPC. The BPC has unique applications in formulations for aquaculture or livestock feed, and other pet food as well as for food formulations intended for human consumption. The sugar co-products, including glucose, have applications in industry and science and are particularly suitable for use as feedstocks for fermentation processes, livestock feeds, or biochemical conversion processes.
Abstract: The present invention provides compositions, methods, and kits based on a novel two-enzyme system. This system uses a combination of an appendage dependent endoxylanase and xylobiohydrolase activity to produce xylobiose and xylan-derived oligosaccharides using lignocellulosic biomass material, an enriched xylan fraction thereof, or an extracted, purified xylan material as a starting material.
Type:
Grant
Filed:
September 8, 2020
Date of Patent:
April 4, 2023
Inventors:
Franz Josef St. John, Merritt Evan Casey Crooks
Abstract: The invention provides compositions and methods for engineering bacteria to produce sialylated and N-acetylglucosamine-containing oligosaccharides, and the use thereof in the prevention or treatment of infection.
Type:
Grant
Filed:
August 28, 2019
Date of Patent:
April 4, 2023
Assignee:
Glycosyn LLC
Inventors:
Massimo Merighi, Matthew Ian Heidtman, John M. McCoy
Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.
Type:
Grant
Filed:
May 27, 2020
Date of Patent:
March 28, 2023
Assignee:
Genomatica, Inc.
Inventors:
Jun Sun, Anthony P. Burgard, Priti Pharkya